Curcumin is a yellow-orange dye widely used as spice, food coloring and preservative. It also exhibits broad range of therapeutic effects against different disorders such cancer, diabetes, or neurodegenerative diseases. As compound insoluble in water curcumin accumulates cell membranes due to this location it may indirectly lead the observed by structurally altering membrane environment. To exert strong structural on needs adopt transbilayer orientation. However, there no agreement...

Abstract The endoperoxides of β-carotene (βCar-EPOs) are regarded as main products the chemical deactivation 1 O 2 by β-carotene, one most important antioxidants, following a concerted singlet-singlet reaction. Here we challenge this view showing that βCar-EPOs formed in absence non-concerted triplet-triplet reaction: 3 + → βCar-EPOs, which manifests strong biradical character. Thus, reactivity towards oxygen is governed its excited triplet state. while being stable dark, photochemically...

Nanodiscs are suitable tools for studies of membrane proteins (MPs) due to their ability mimic native biological membranes, and several MP structures solved in nanodiscs. Among the various cell components, cholesterol (CHL) is known regulate protein function its concentration can reach up 50 mol %. However, comprising challenging hydrophobic nature, hence, nanodiscs with only a low have been studied. To overcome problem, analogs high solubility polar solutions often used, one them...

Curcumin is a plant-derived yellow-orange compound widely used as spice, dye and food additive. It also believed to have therapeutic effects against different disorders. On the other hand, there are data showing its phototoxicity bacteria, fungi various mammalian cells. Since mechanism of phototoxic action not fully understood, we investigated here potential curcumin in liposomal model membranes HaCaT First, detection singlet oxygen (1 O2 ) luminescence proved that generates 1 upon blue...

It was shown that in membranes containing raft domains, the macular xanthophylls lutein and zeaxanthin are not distributed uniformly, but excluded from saturated domains about ten times more concentrated unsaturated bulk lipids. The selective accumulation of direct proximity to lipids, which especially susceptible lipid peroxidation, could be very important as far their antioxidant activity is concerned. Therefore, protective role against peroxidation investigated made raft-forming mixtures...

Abstract The membranes of retina photoreceptors have unique lipid composition. They contain a high concentration polyunsaturated docosahexaenoic acid, with six double bonds, and are enriched in phosphatidylethanolamines. Based on their phospholipid composition cholesterol content, can be divided into three types: plasma membrane, young disks membranes, old membranes. High amount abundant illumination, respiratory demands make these sensitive to oxidative stress peroxidation. Human retinas...

Lateral organization of membranes made from binary mixtures dimyristoylphosphatidylcholine (DMPC) or dipalmitoylphosphatidylcholine (DPPC) and macular xanthophylls (lutein zeaxanthin) was investigated using the saturation-recovery (SR) EPR spin-labeling discrimination by oxygen transport (DOT) method in which bimolecular collision rate molecular with nitroxide spin label is measured. This work undertaken to examine whether not lutein zeaxanthin, that parallel cholesterol its function as a...

Photoreceptor membranes have a unique lipid composition. They contain high level of polyunsaturated fatty acids including the most unsaturated acid in nature, docosahexaenoic (22:6), and are enriched phosphatidylethanolamines. The phospholipid composition cholesterol content subcellular components photoreceptor outer segments enables to divide into three types: plasma membranes, young disc old membranes. A degree unsaturation, extended exposure intensive irradiation, respiratory demands make...

This review focuses on modern spectroscopic approaches to the investigations of incorporation and organization carotenoids in biological membranes experimental models them. In particular, application electronic absorption, emission, Raman, resonance Raman electron paramagnetic spectroscopies are discussed light molecular lipid membranes. The perspectives prospects exploiting energy transfer processes involving this context. intermolecular interactions within depend two major factors. One is...

Neuroleptic drugs are widely applied in effective treatment of schizophrenia and related disorders. The lipophilic character neuroleptics means that they tend to accumulate the lipid membranes, impacting their functioning processing. In this paper, effect four drugs, namely, thioridazine, olanzapine, sulpiride, amisulpride, on neutral negatively charged bilayers was examined. interaction with lipids subsequent changes membrane physical properties assessed using several complementary...

Tauroursodeoxycholic acid (TUDCA), a hydrophilic bile containing taurine conjugated with the ursodeoxycholic (UDCA), has been known and used from ancient times as therapeutic compound in traditional Chinese medicine. TUDCA recently gaining significant interest neuroprotective agent, also exploited visual disorders. Among several mechanisms of TUDCA’s protective action, its antioxidant activity stabilizing effect on mitochondrial plasma membranes are considered. In this work we investigated...

Cr(vi) is a harmful, carcinogenic agent with high permeability rate throughout the lipid membranes. In an intracellular environment and during interactions cellular membranes, it undergoes instant reduction to lower oxidation states radical states, recognized as most dangerous factor for cells. The membrane visible organelle in interior exterior of cell. this study, liposomes non-lamellar inverted hexagonal phase structures based on phosphoethanolamine (PE) were used model bilayers because...

This review focuses on modern spectroscopic approaches to the investigations of incorporation and organization carotenoids in biological membranes experimental models them. In particular, application electronic absorption, emission, Raman, resonance Raman electron paramagnetic spectroscopies are discussed light molecular lipid membranes. The perspectives prospects exploiting energy transfer processes involving this context. intermolecular interactions within depend two major factors. One is...