A5089673359- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Treatment of Major Depression
- Tryptophan and brain disorders
- Proteins in Food Systems
- Adipose Tissue and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Lipid Membrane Structure and Behavior
- Nuclear Receptors and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Ion channel regulation and function
- Nerve injury and regeneration
- Neuroendocrine regulation and behavior
- Pharmacological Effects and Assays
- Adenosine and Purinergic Signaling
- Genomics and Chromatin Dynamics
- Neurological disorders and treatments
- Genetics and Neurodevelopmental Disorders
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Protein Kinase Regulation and GTPase Signaling
Polish Academy of Sciences
2015-2024
Maj Institute of Pharmacology
2015-2024
Jagiellonian University
2015-2024
University of Arkansas System
2017
University of Kentucky
2015-2017
University of Agriculture in Krakow
2015-2017
University of Łódź
2017
Institute of Pharmacology
2012
Polish Academy of Learning
2010
Lundbeck (Denmark)
2006
Sample preparation is a fundamental step in proteomic methodologies. The quality of the results from experiment dependent on nature sample and properties proteins. In this study, various pre-treatment methods were compared by analysis; we analysed rat brain structures after chloroform/methanol, acetone, TCA/acetone TCA protein precipitation procedures. content supernatant was also examined 2-DE. We found that for four structures, with chloroform/methanol acetone delivered highest recovery...
Abstract Lactoglobulin is a natural protein present in bovine milk and common component of human diet, known for binding with high affinity wide range hydrophobic compounds, among them fatty acids 12–20 carbon atoms long. Shorter were reported as not to β‐lactoglobulin. We used X‐ray crystallography fluorescence spectroscopy show that lactoglobulin binds also 8‐ 10‐carbon caprylic capric acids, however lower affinity. The determined apparent association constant complex acid 10.8 ± 1.7 × 10...
An investigation of the interactions between bio-polymeric nanoparticles (NPs) and RAW 264.7 mouse murine macrophage cell line has been presented. The viability, immunological response, endocytosis efficiency NPs were studied. Biopolymeric synthesized from a nanoemulsion using phase inversion composition (PIC) technique. two types biopolymeric that obtained consisted biocompatible polymer, polycaprolactone (PCL), either with or without its copolymer poly(ethylene glycol) (PCL-b-PEG). Both...
Numerous studies highlight the therapeutic potential of G protein-coupled receptor (GPCR) heterodimers, emphasizing their significance in various pathological contexts. Despite extensive basic research and promising outcomes animal models, translation GPCR heterodimer-targeting drugs into clinical use remains limited. The complexities vivo conditions, particularly within thecomplex central nervous system, pose challenges fully replicating physiological environments, hindering success. This...
The serotonin 5-HT1A receptor (5-HT1 A R) and dopamine D2 (D2 have been implicated as important sites of action in antipsychotics. Several lines evidence indicate the key role G protein-coupled receptors (GPCRs) heteromers pathophysiology schizophrenia highlight these complexes novel drug targets. Because heterodimers can form only on those cells co-expressing constituent receptors, they present a target high pharmacological specificity context biochemical effects induced by antipsychotic...
The use of polymeric nanoparticles (NPs) in pharmacology provides many benefits because this approach can increase the efficacy and selectivity active compounds. However, development new nanocarriers requires better understanding interactions between NPs immune system, allowing for optimization NP properties effective drug delivery. Therefore, present study, we focused on investigation biocompatible a murine macrophage cell line (RAW 264.7) human monocytic leukemia (THP-1). based liquid core...
Abstract Rationale Our study aimed to unravel the unknown mechanisms behind exceptional efficacy of Psilocybin (PSI) in treating treatment-resistant depression (TRD). Focusing on Wistar-Kyoto (WKY) rats with a TRD phenotype and Wistar (WIS) as normative comparison, we investigated behavioral neuroplasticity-related responses PSI, striving shed light distinctive features its antidepressant effects. Objectives We set out assess impact acute prolonged PSI administration WKY WIS rats, employing...
This study used in-situ hybridization to examine D1 and D2 receptor mRNA expression in the brains of rats subjected chronic mild stress and/or treatment (5 weeks) with antidepressant drugs imipramine fluoxetine. As previous studies using procedure, decreased consumption a palatable sucrose solution, both antidepressants reversed this effect. In-situ probes was studied midbrain forebrain sections. In sections, message substantia nigra lateral part ventral tegmental area (VTA), but not medial...
Few studies have investigated neurobiological and biochemical differences between stress-resilient stress-vulnerable experimental animals.We alterations in mesolimbic dopamine D2 receptor density mRNA expression level stressed rats at two time points, i.e. after 2 5 weeks of chronic mild stress (CMS).We used the paradigm because it is a well-established animal model depression. Two groups were distinguished during CMS experiments: (1) reactive (70 %), which displayed decrease drinking...
Evidence for hetero-oligomerization has recently been provided various G protein-coupled receptors. In this paper, we have studied the possibility that dopamine D1 and D2 receptors physically interact with each other. Human were fluorescently tagged derivatives of green fluorescence protein transiently coexpressed in membrane human embryonic kidney 293 cells. Using qualitative spectroscopy, as well quantitative Förster resonance energy transfer (FRET) analysis, performed a single cell by...
Prolonged stress perturbs physiological balance of a subject and thus can lead to depression. Nevertheless, some individuals are more resilient than the others. Defining molecular factors underlying resilience may contribute development new antidepressant strategy based on restoration phenotype in depressed subjects. We used chronic mild (CMS) paradigm-well-characterized animal model depression which caused rats behavioral deficits (anhedonia) manifested by decreased consumption sucrose...