A5075668441- X-ray Spectroscopy and Fluorescence Analysis
- Spectroscopy Techniques in Biomedical and Chemical Research
- Trace Elements in Health
- Nuclear Physics and Applications
- Spectroscopy and Chemometric Analyses
- Electron and X-Ray Spectroscopy Techniques
- Advanced X-ray Imaging Techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Radiation Effects and Dosimetry
- Advanced biosensing and bioanalysis techniques
- thermodynamics and calorimetric analyses
- Radiation Therapy and Dosimetry
- Radiation Shielding Materials Analysis
- Bee Products Chemical Analysis
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Metal complexes synthesis and properties
- Geology and Environmental Impact Studies
- Metabolomics and Mass Spectrometry Studies
- Prion Diseases and Protein Misfolding
- Vanadium and Halogenation Chemistry
- Radioactive element chemistry and processing
- DNA Repair Mechanisms
- X-ray Diffraction in Crystallography
- Heavy Metals in Plants
Institute of Nuclear Physics, Polish Academy of Sciences
2015-2024
Polish Academy of Sciences
2005-2020
Czech Academy of Sciences, Institute of Physics
2020
Krakow Cardiovascular Research Institute
2018-2020
GTx (United States)
2018
John Wiley & Sons (United States)
2017
Interface (United Kingdom)
2017
Jagiellonian University
2007
Brookhaven National Laboratory
1987-1990
Abstract The SOLARIS synchrotron located in Krakow, Poland, is a third-generation light source operating at medium electron energy. first was observed 2015, and the consequent development of infrastructure lead to users’ experiments soft X-ray energies 2018. Presently, expands its operation towards hard X-rays with continuous developments beamlines concurrent infrastructure. In following, we will summarize design, describe research together main performance parameters, upgrade, plans.
Abstract DNA double strand breaks (DSBs) are deadly lesions that can lead to genetic defects and cell apoptosis. Techniques directly detect DSBs include scanning electron microscopy, atomic force microscopy (AFM), fluorescence based approaches. While these techniques be used identify they provide no information on the molecular events occurring at break. Tip‐enhanced Raman scattering (TERS) from nanoscale in combination with AFM provides a new way visualize characterize structure of DSBs....
Lipid droplets (LDs) are key organelles in cancer cells proliferation, growth, and response to stress. These nanometric structures can aggregate reach the size of microns becoming important cell components. Although it is known that LDs contain various lipids, their chemical composition still under investigation. Moreover, function cell's exogenous factors also not fully understood. Raman spectroscopy, together with chemometrics, has been shown be a powerful tool for analytical analyses...
Abstract The recent emergence of High Definition (HD) FT-IR and Quantum Cascade Laser (QCL) Microscopes elevated the IR imaging field very close to clinical timescales. However, speed acquisition data quality are still critical factors in reaching clinic. Denoising offers aide both aspects if performed properly. there is a lack direct comparison efficiency denoising techniques general. To achieve such within rigorous framework obtaining information about signal loss, simulated dataset...
Abstract Spore-forming bacteria are a class of microorganisms that possess the ability to survive in extreme environmental conditions. Morphological features spores assure their resistance stress factors such as high temperature, radiation, disinfectants, and drying. Consequently, spore elimination industrial medical environments is very challenging. Ceragenins new cationic lipids characterized by broad spectrum bactericidal activity resulting from amphipathic nature membrane-permeabilizing...
Abstract OLED technology beyond small or expensive devices requires light-emitters, luminophores, based on earth-abundant elements. Understanding and experimental verification of charge transfer in luminophores are needed for this development. An organometallic multicore Cu complex comprising Cu–C Cu–P bonds represents an underexplored type luminophore. To investigate the structural rearrangements material, we apply complementary pump-probe X-ray techniques: absorption, emission, scattering...
Melanocytes exposed to artificial sunlight and analysed with FTIR Raman spectroscopy show changes in DNA bands evidence of lipid accumulation.
The lack of molecular mechanistic understanding the interaction between metal complexes and biomolecules hampers their potential medical use. Herein we present a robust procedure combining resonant X-ray emission spectroscopy multiscale dynamics simulations, which allows for straightforward elucidation precise mechanism at atomic level. report unveils an unforeseen hydrolysis process DNA binding [Pt{N(p-HC6F4)CH2}2py2] (Pt103), showed cytotoxic activity in past. Pt103 preferentially...
Atomic force microscopy - infrared (AFM-IR) spectroscopy allows spectroscopic studies in the mid-infrared (mid-IR) spectral region with a spatial resolution better than is allowed by diffraction limit. We show that high can be used to perform and imaging at subcellular level fixed eukaryotic cells. collect AFM-IR images of structures include lipid droplets, vesicles cytoskeletal filaments, relying on intrinsic contrast from IR light absorption. also obtain absorption spectra individual...
Nowadays, it is well established that changes of cell stiffness observed by atomic force microscopy are linked with the cytoskeleton.Its structural and functional alterations underlying major diseases such as cancer, inflammation or neurodegenerative disorders.So far, use mostly focused on determination Young modulus using modified Hertz model.It can quantitatively describe elastic properties living cells, however, its value burdened fact cells neither isotropic nor homogeneous...
The causes of prostate cancer are still obscure but some evidence indicates that there is a close connection between several trace elements and processes which may lead to malignant cells. In our study the microbeam synchrotron radiation X-ray fluorescence emission (micro-SRIXE) technique was applied for quantitative analysis selected elements. For first time, we correlate concentrations Mn, Fe, Cu, Zn with clinical stage at time operation (described by Gleason grade). Serial sections...
Understanding the response of cancer cells to ionising radiation is a crucial step in modern radiotherapy. Raman microspectroscopy, together with Partial Least Squares Regression (PLSR) analysis has been shown be powerful tool for monitoring biochemical changes irradiated on subcellular level. However, date, majority studies have performed using single spectrum per cell, giving limited view total cell. In current study, mapping whole cell area was undertaken ensure more comprehensive...
Abstract The application of metal nanoparticles as an efficient drug delivery system is one the directions cancer therapy development. However, this strategy requires precise information about how interacts with applied nanocarrier. In study, atomic force microscopy combined infrared spectroscopy (AFM-IR) was used for first time to investigate erlotinib adsorption structure on two different types 15 nm nanoparticle mono-layers, namely, silver (AgNP) and gold (AuNP) mono-layers. Because are...
Fourier transform infrared spectroscopy has emerged as a powerful tool for tissue specimen investigation. Its nondestructive and label-free character enables direct determination of biochemical composition samples. Furthermore, the introduction polarization enriches this technique by possibility molecular orientation study apart from purely quantitative analysis. Most studies focused on polymer samples with well-defined axis. Here, four-polarization approach Herman's in-plane function...