- Spectroscopy Techniques in Biomedical and Chemical Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Spectroscopy and Chemometric Analyses
- thermodynamics and calorimetric analyses
- Archaeology and ancient environmental studies
- Pleistocene-Era Hominins and Archaeology
- Microbial Community Ecology and Physiology
- Nanoparticles: synthesis and applications
- Genomics and Phylogenetic Studies
- Cultural Heritage Materials Analysis
- Metal-Organic Frameworks: Synthesis and Applications
- Methane Hydrates and Related Phenomena
- Molecular Biology Techniques and Applications
- Microplastics and Plastic Pollution
- Calcium Carbonate Crystallization and Inhibition
- Conservation Techniques and Studies
- Geology and Paleoclimatology Research
- 3D Printing in Biomedical Research
- Heavy Metals in Plants
- Building materials and conservation
- X-ray Spectroscopy and Fluorescence Analysis
- Lignin and Wood Chemistry
- Clay minerals and soil interactions
- Advanced X-ray Imaging Techniques
Elettra-Sincrotrone Trieste S.C.p.A.
2015-2024
Lawrence Berkeley National Laboratory
2012-2020
Elucid Bioimaging
2018
Berkeley College
2014
University of California, Berkeley
2013
AREA Science Park
2009-2011
University of Trieste
2007-2010
Bacteria from phyla lacking cultivated representatives are widespread in natural systems and some have very small genomes. Here we test the hypothesis that these cells thus might be enriched by filtration for coupled genomic ultrastructural characterization. Metagenomic analysis of groundwater passed through a ~0.2-μm filter reveals wide diversity bacteria WWE3, OP11 OD1 candidate phyla. Cryogenic transmission electron microscopy demonstrates that, despite morphological variation,...
Modern hyperspectral imaging techniques can easily satisfy the representative statistical sampling requirements for any study providing large volume datasets. The job of understanding collected...
There is evidence and serious concern that microplastics have reached the most remote regions of planet, but how far they travelled in terrestrial ecosystems? This study presents first field-based plastic ingestion by a common central component Antarctic food webs, collembolan Cryptopygus antarcticus . A large piece polystyrene (PS) foam (34 × 31 5 cm) covered microalgae, moss, lichens microfauna was found fellfield along shores Fildes Peninsula (King George Island). The application an...
The human skin microbiome acts as an important barrier protecting our body from pathogens and other environmental influences. Recent investigations have provided evidence that Archaea are a constant but highly variable component of the microbiome, yet factors determine their abundance changes unknown. Here, we tested hypothesis archaea on is influenced by age physiology quantitative PCR 51 different samples taken subjects various age. Our results reveal more abundant in either older than 60...
Until nowadays most infrared microspectroscopy (IRMS) experiments on biological specimens (i.e., tissues or cells) have been routinely carried out fixed dried samples in order to circumvent water absorption problems. In this paper, we demonstrate the possibility widen range of in-vitro IRMS vibrational analysis live cellular samples, thanks development novel biocompatible IR-visible transparent microfluidic devices (MD). highlight relevance MD (MD-IRMS), performed a systematic exploration...
Polyfurfuryl alcohol (PFA) is one of the most intriguing polymers because, despite its easy polymerization in acid environment, molecular structure definitely not obvious. Many studies have been performed recent decades, and every time, surprising aspects came out. With present study, we aim to take advantage all findings previous investigations exploit them for interpretation completely cured PFA spectra registered with three powerful techniques characterization solid, insoluble polymers:...
Here we present a new bonding protocol for SU-8 negative tone photoresist that exploits the chemical modifications induced in resin by exposure to 254 nm (UVC) light. Fourier Transform Infrared microspectroscopy (μ-FTIR) was used carry out thorough study on processes and occurring within epoxy 365 In particular, established UVC light promotes opening of rings bypassing post-exposure bake. The possibility promote further activation resin, already patterned with standard UV lithography,...
Abstract Sediment-hosted CO2-rich aquifers deep below the Colorado Plateau (USA) contain a remarkable diversity of uncultivated microorganisms, including Candidate Phyla Radiation (CPR) bacteria that are putative symbionts unable to synthesize membrane lipids. The origin organic carbon in these ecosystems is unknown and source CPR lipids remains elusive. We collected cells from groundwater brought surface by eruptions Crystal Geyser, sequenced community, analyzed whole community lipidome...
A long-standing desire in biological and biomedical sciences is to be able probe cellular chemistry as processes are happening inside living cells. Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy a label-free nondestructive analytical technique that can provide spatiotemporal distributions relative abundances of biomolecules specimen by their characteristic vibrational modes. Despite great progress recent years, SR-FTIR imaging systems remains challenging...
The actual chemical structure of polyfurfuryl alcohol (PFA) is still uncertain in spite several studies on the topic, variations during polymerization processes being one reason that must be addressed. use a limited set analytical techniques often insufficient to provide an exhaustive characterization. Moreover, it not possible exactly determine presence and amount each specific functional group polymeric structure. We employed both Fourier Transform Infrared Spectroscopy (FTIR) Resonant...
Herein, we propose eco-friendly electrolytes based on sodium chloride as a hydrogen bond acceptor and glycerol donor, alternatives to toxic, flammable unsustainable commonly...
In recent years, peptide-based nanomaterials gain significant attention in drug discovery due to their promising functionality biophysical processes and low production costs. Among synthetic short peptides, diphenylalanine (Phe-Phe), a...
Aimed at developing accurate, reliable and cost-saving analytical techniques for drugs screening we evaluated the potential of Fourier Transform (FT) InfraRed (IR) microspectroscopy (microFTIR) as a quantitative pre-diagnostic approach rapid identification IR signatures targeting specific molecular pathways causing Chronic Myeloid Leukemia (CML). To obtain reproducible FTIR absorbance spectra necessary spatial resolution optimized sample preparation acquisition parameters on single channel...
The release of nanoplastics (NPs) in the environment is a significant health concern for long-term exposed humans. Although their usage has certainly revolutionized several application fields, at nanometer size, NPs can easily interact cellular level, resulting potential harmful effects. Micro/Nanoplastics (M/NPs) have demonstrated impact on mammalian endocrine components, such as thyroid, adrenal gland, testes, and ovaries, while more investigations prenatal postnatal exposure are urgently...
Earth harbors an enormous portion of subsurface microbial life, whose microbiome flux across geographical locations remains mainly unexplored due to difficult access samples. Here, we investigated the relatedness biofilms two sulfidic springs in southeast Germany that have similar physical and chemical parameters are fed by one deep groundwater current. Due their unique hydrogeological setting these provide accessible windows dominated same uncultivated archaeal species, called SM1...