A5090828925- Extracellular vesicles in disease
- Nanopore and Nanochannel Transport Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Thermal Regulation in Medicine
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Animal Nutrition and Physiology
- Tendon Structure and Treatment
- melanin and skin pigmentation
- Exercise and Physiological Responses
- Stress Responses and Cortisol
- Retinal and Optic Conditions
- Herpesvirus Infections and Treatments
- Peptidase Inhibition and Analysis
- Aerosol Filtration and Electrostatic Precipitation
- Cardiovascular Disease and Adiposity
- Retinal Development and Disorders
- Tryptophan and brain disorders
- Blood properties and coagulation
- Trace Elements in Health
- Circadian rhythm and melatonin
- Inhalation and Respiratory Drug Delivery
- Treatment of Major Depression
- Nanoplatforms for cancer theranostics
- Blood Coagulation and Thrombosis Mechanisms
Jagiellonian University
2016-2025
Institute of Physics
2016-2025
Cardinal Stefan Wyszyński University in Warsaw
2018-2024
This study compares nanoLC-MALDI-MS/MS and nanoLC-TIMS-MS/MS in analyzing EVs from BALF of asthma IPF patients, emphasizing EVs' roles inflammation, signaling pathways, their potential as diagnostic biomarkers therapeutic targets.
Ectosomes (Ects) are a subpopulation of extracellular vesicles formed by the process plasma membrane shedding. In present study, we profiled ectosome-specific microRNAs (miRNAs) in patients with type 2 diabetes mellitus (T2DM) and analyzed their pro- anti-angiogenic potential. Methods: We used different approaches for detecting enumerating Ects, including atomic force microscopy, cryogenic transmission electron nanoparticle tracking analysis. Furthermore, bioinformatics tools to analyze...
The aim of this study was to investigate the role urine-derived extracellular vesicles (uEVs) in diabetic kidney disease (DKD) patients diagnosed with type 2 diabetes mellitus (T2DM).UEVs were characterized by size distribution and microRNA content next-generation small RNA sequencing quantitative reverse transcription PCR.A subset sixteen miRNAs enriched T2DM DKD, including hsa-miR-514a-5p, hsa-miR‑451a, hsa-miR-126-3p, hsa-miR-214, or hsa-miR‑503 identified. Eight as hsa-miR-21-3p,...
Melanoma cells release extracellular vesicles (EVs) subpopulations which differ in size, phenotype and molecular content. derived EVs play a role the development progression of cancer by delivering surface receptors bioactive (proteins, lipids, nucleic acids) or signaling molecules to target cells. We applied Fourier Transform Infrared Spectroscopy (FTIR) compare infrared spectra absorption for different originating from normal human melanocytes, primary cutaneous melanoma (WM115) metastatic...
Platelet extracellular vesicles (PEVs) are emerging as key biomarkers in diabetes mellitus (DM), reflecting altered platelet function and coagulation pathways. This study compares two proteomic techniques—nanoLC-MALDI-MS/MS nanoLC-TIMS-MS/MS—for analyzing PEVs diabetic patients, to assess their potential for biomarker discovery. were isolated from platelet-rich plasma characterized using tunable resistive pulse sensing (TRPS), Fourier-transform infrared (FTIR) spectroscopy, transmission...
The aim of this study was to check the relationship between density urinary EVs, their size distribution, and progress early renal damage in type 2 diabetic patients (DMt2). Patients were enrolled study, glycated hemoglobin (HbA1c) below 7% a threshold for properly controlled (CD) poorly (UD). further divided into two groups: without failure (NRF) with (RF) according Glomerular Filtration Rate. Density diameter EVs determined by Tunable Resistive Pulse Sensing. Additionally, visualized means...
In this study, we verified the hypothesis that Raman signature of urinary extracellular vesicles (UEVs) can be used to stratify patients with diabetes at various stages chronic kidney disease (CKD). Patients type 2 diagnosed different CKD and healthy subjects were enrolled in study. UEVs isolated using low-vacuum filtration followed by ultracentrifugation. Correlation analysis, multiple linear regression principal component analysis find differences between spectral fingerprints derived from...
The aim of the study was to estimate influence different levels Cu, Zn, and Mn nanoparticles on activity aminopeptidases in turkey. An experiment carried out 144 turkey hen Hybrid Converter. birds were divided into groups given standard- nanoparticle-supplementation level copper (Cu 20, 10, 2 mg/kg), zinc (Zn 100, 50, 10 ppm), manganese (Mn covering respectively 100%, 50%, 10% physiological demands for those minerals diet. (alanyl: AlaAP, leucyl: LeuAP arginyl: ArgAP) after supplementation...
Kynurenines, products of tryptophan (TRP) metabolism, display neurotoxic (eg. 3-hydroxykynurenine; 3-HK) or neuroprotective kynurenic acid; KYNA) properties. Imbalance between the enzymes constituting kynurenine pathway (KP) plays a role in several disease, including neurodegeneration. In this study, we track changes concentrations and its selected metabolites after damage to retinal ganglion cells link data with expression KP enzymes. Brown-Norway rats were subjected intravitreal...
Recent years have brought great focus on the development of drug delivery systems based extracellular vesicles (EVs). Considering possible applications EVs as carriers, isolation process is a crucial step. To solve problems involved in EV isolation, we developed and validated new method—low-vacuum filtration (LVF)—and compared it with two commonly applied procedures—differential centrifugation (DC) ultracentrifugation (UC). isolated from endothelial cell culture media were characterized by...
Among the various biomarkers that are used to diagnose or monitor disease, extracellular vesicles (EVs) represent one of most promising targets in development new therapeutic strategies and application diagnostic methods. The detection circulating platelet-derived microvesicles (PMVs) is a considerable challenge for laboratory diagnostics, especially preliminary phase disease. In this study, we present multistep approach immobilizing detecting PMVs biological samples (microvesicles generated...
For this study, we tested and optimized silicon surface functionalization procedures for capturing urinary extracellular vesicles (uEVs). The influence of the silane type (APTES or GOPS) protein concentration on efficiency uEVs binding was investigated. Human lactadherin (LACT) used to capture uEVs. We applied characterization techniques, including ellipsometry, atomic force microscopy, time-of-flight secondary ion mass spectrometry, observe changes in biosensor after each step. were...
Recent years brought great focus in the field of development extracellular vesicles (EVs) based drug-delivery systems. Considering possible applications EVs as a drug carriers isolation process is crucial step. To solve problems related with EV isolation, we created and validated new method – Low Vacuum Filtration (LVF) compared it two commonly applied procedures - differential centrifugation (DC) ultracentrifugation (UC). isolated from endothelial cells culture media have been...
Abstract The aim of the study was to assess activity lysosomal enzymes: aminopeptidases, including alanine aminopeptidase (AlaAP), leucine (LeuAP), arginine (ArgAP), and glycosidases, such as β-galactosidase (BGAL), β-glucuronidase (BGRD), β-glucosidase (BGLU), N-acetyl-β-hexosaminidase (HEX), α-glucosidase (AGLU) α-mannosidase (MAN) in liver ostriches (n = 80) fed diet supplemented with linseed (4% 8%) rapeseed (5% 10%), low high level vitamin E. (40 100 mg). results indicate that higher E...
Diabetes mellitus (DM) is a prevalent metabolic disease. The clinical impact of sustained hyperglycemia on ligament healing has not been well characterized. known cause macro-, microvascular, and diabetic ulcer difficulties among tissues. Therefore, we aimed to investigate the potential occurring in injured healthy ligaments individuals using rat model. We hypothesize that DM may contribute altering knee medial collateral (MCL), thus its morphology, biochemical fitness, functionality. study...
BACKGROUND Necrotizing herpetic retinopathies associated with varicella zoster virus (VZV) occurs sporadically and typically presents acute retinal necrosis (ARN) features. This report an incident of ARN in immunocompetent patient who was referred to the hospital for vision loss. CASE REPORT A 56-year-old previously healthy man presented Emergency Department loss his left eye. Four weeks before admission, he had upper respiratory tract infection received a diagnosis VZV infection, based on...
Abstract Microvesicles (MVs) are found in several types of body fluids and promising disease biomarkers therapeutic targets. This study aimed to develop a novel biofunctionalized surface for binding plasma microvesicles (PMVs) based on lab-on-a-chip (LOC) approach. A new lactadherin (LACT)-functionalized was prepared examined monitoring PMVs. Moreover, two different strategies LACT immobilization silicon were applied compare orientations. higher PMV efficiency observed bonded an αvβ3...