A5060070398- Extracellular vesicles in disease
- Antimicrobial Resistance in Staphylococcus
- Platelet Disorders and Treatments
- Clostridium difficile and Clostridium perfringens research
- Streptococcal Infections and Treatments
- Intestinal Malrotation and Obstruction Disorders
- Cardiovascular Disease and Adiposity
- Epilepsy research and treatment
- Intestinal and Peritoneal Adhesions
- Heavy Metals in Plants
- Advanced Proteomics Techniques and Applications
- Plant responses to water stress
- Bacteriophages and microbial interactions
- Nicotinic Acetylcholine Receptors Study
- Inhalation and Respiratory Drug Delivery
- Cancer Treatment and Pharmacology
- Bacillus and Francisella bacterial research
- Plant Stress Responses and Tolerance
- Pharmacological Receptor Mechanisms and Effects
- Paraoxonase enzyme and polymorphisms
- Biosensors and Analytical Detection
- Lichen and fungal ecology
- Acoustic Wave Resonator Technologies
- Cynara cardunculus studies
- Neuroscience and Neuropharmacology Research
Jagiellonian University
2016-2025
Gdańsk Medical University
2013-2014
This study compares nanoLC-MALDI-MS/MS and nanoLC-TIMS-MS/MS in analyzing EVs from BALF of asthma IPF patients, emphasizing EVs' roles inflammation, signaling pathways, their potential as diagnostic biomarkers therapeutic targets.
Platelet extracellular vesicles (PEVs) are emerging as key biomarkers in diabetes mellitus (DM), reflecting altered platelet function and coagulation pathways. This study compares two proteomic techniques—nanoLC-MALDI-MS/MS nanoLC-TIMS-MS/MS—for analyzing PEVs diabetic patients, to assess their potential for biomarker discovery. were isolated from platelet-rich plasma characterized using tunable resistive pulse sensing (TRPS), Fourier-transform infrared (FTIR) spectroscopy, transmission...
The extracellular vesicles (EVs) released by plant pathogens of the Pectobacterium genus were investigated. isolates obtained using differential centrifugation followed filtration and characterized in terms total protein content particle size distribution. transmission electron microscopy (TEM) analysis revealed presence two morphologically differentiated subpopulations isolates. proteomic matrix-assisted laser desorption ionization mass spectrometry with time flight detector...
The aim of this study was to check the relationship between density urinary EVs, their size distribution, and progress early renal damage in type 2 diabetic patients (DMt2). Patients were enrolled study, glycated hemoglobin (HbA1c) below 7% a threshold for properly controlled (CD) poorly (UD). further divided into two groups: without failure (NRF) with (RF) according Glomerular Filtration Rate. Density diameter EVs determined by Tunable Resistive Pulse Sensing. Additionally, visualized means...
Staphylococcus aureus is a leading cause of bloodstream infections (BSI) and diseases that may be caused by hematogenous spread. The staphylococcal adhesin, for which the association with emerging as complication septicemia has been well documented, bone sialoprotein-binding protein (Bbp). aim study was to assess prevalence bbp gene in S. isolates associated BSI investigate what degree distribution this linked clonality population. Spa typing, used order explore genetic population structure...
Hepatocellular damage by the harmful effects of xenobiotics, which increase production free radicals, is a widespread phenomenon. The extract from leaves Cynara scolymus L. available as an artichoke preparation (natural source) antioxidants may serve potential hepatoprotective factor. This study aimed to evaluate impact protective and regenerative properties on liver in three doses: 0.5; 1.0; 1.5 g/kg bw/day. evaluation was conducted measuring levels oxidative stress parameters, including...
One of the most important issues considered in ecotoxicology is elevated accumulation different elements soil as a consequence anthropogenic activities.In this paper, determination nine obtained from three forest complexes (sycamore, beech, and spruce) urbanized non-urbanized regions Tatra National Park presented.Additionally, comprehensive presentation detailed results correlation analyses between individual accordance to each valley, well selected species trees, shown.Speciation studies...
Abstract Alcohol and nicotine (tobacco smoke) are often used together, taking both addictive substances is associated with an increased risk of certain diseases. It extremely important to understand the pharmacodynamic pharmacokinetic mechanisms interaction between ethanol, which still not fully understood. The study aimed evaluate influence chronic alcohol consumption on biotransformation in ethanol-preferring non-preferring male female rats. Rats were divided into four groups depending...
The aim of our study was to assess the possibility predicting Enterococcus faecalis pathogenicity based on morphological changes monocytes interacting with bacteria and their adherence biofilm. Changes in size granularity monocytes, as well biofilm were assessed using FACScan flow cytometer after 1h co-incubation enterococcal 37°C. obtained results validated respect incubated without bacteria. most prominent observed case commensal interaction isolated from blood stream urine caused...
There is a global need to explore alternative protein sources and develop new health-promoting plant-based products. This study aimed produce beverages from organic seeds of chickpeas green or red lentils assess the potential using L. plantarum 299v ferment these beverages. Spectrophotometric methods were used evaluate ability neutralize 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH•) chelate Fe2+. Total dietary fiber (including soluble insoluble fractions) profiles compared among tested...
Recently, the proteomic analysis has become an ideal tool to study structure and function of platelets. We proposed a nano-liquid chromatography (nano-LC) technique coupled off-line with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) for rat platelet proteome analysis. In this study, we attempted analyze in two different subcellular fractions: cytosol membrane. Platelet-rich plasma was collected from healthy rats. The samples were extracted...
During last decades C. difficile infection (CDI) has emerged as a most frequent health-care associated cause of morbidity and mortality in developed world. In Europe about 40,000 patients die each year CDI, Poland rapid increase CDI rates been observed, reaching appr. 11,500 hospitalized cases 2013.
Abstract Sulthiame is an old antiepileptic medicine with controversial history, whose effectiveness and safety in use have been stated some current studies. However, there still a need for further clinical examinations confirmation of its usefulness tolerability monotherapy add‐on therapy epilepsy various etiologies. A fully validated RP HPLC–UV method determination sulthiame serum/plasma samples using desethylatrazine as the internal standard was developed. The biological fluid prepared...
In this article, we have presented the development and validation of a rapid sensitive reversed-phase liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for determination vincristine (VCR) in patient serum samples. Chromatographic separation was achieved on Kinetex® (Singapore) column using mobile phase consisting 25 mM acetic acid 0.3% formic (A) methanol (B) gradient elution mode at flow rate 0.3 mL/min. The VCR internal standard (vinblastine) were monitored multiple...