Enterococcus faecalis is a bacterium which accompanies us from the first days of our life. As commensal it produces vitamins, metabolizes nutrients, and maintains intestinal pH. All that happens in exchange for niche to inhabit. It not surprising then, was used as an element many probiotics its positive impact on human immune system body general hard ignore. This has also dark side though. The plasticity relative ease with one acquires virulence traits, ability hide or even deceive use...

Background/Objectives: According to the One Health concept, wild birds can be indicators of ecosystem pollution and disease incidence. Escherichia coli strains are widespread worldwide, but there still few reports on association human infections with a potential reservoir highly pathogenic in birds. Fecal E. uropathogenic (UPEC) transmitted between humans may risk factor for urinary tract (UTIs). Results: The results showed that above 50% isolates were grouped as pathogenic, according...

We used flow cytometry to compare the phagocytic activity of monocytes against Staphylococcus aureus strains (both biofilm and planktonic cells) isolated from denture wearers non-wearers. Staphylococcal were cultured in Brain Heart Infusion broth both form stained with a fluorescent reporter (propidium iodide) incubated monocytes. The fluorescence containing phagocytized bacteria was determined by normalized that bacterial experiment. caused greater activation but less prone phagocytosis....

Pandemics from viral respiratory tract infections in the 20th and early 21st centuries were associated with high mortality, which was not always caused by a primary infection. It has been observed that severe course of infection, complications mortality often result co-infection other pathogens, especially Staphylococcus aureus. During COVID-19 pandemic, it also noticed patients infected S. aureus had significantly higher rate (61.7%) compared to SARS-CoV-2 alone. Our previous studies have...

The aim of the study was to evaluate changes in expression PBP5 gene associated with immunosuppression. A linear locked nucleic acid (LNA) probe used measure resistance by Flow-FISH method. Expression measured higher enterococcal strains isolated from renal transplantation (RTx) recipients than commensal strains. Additionally, contrast isolates RTx patients, 17.45% biofilms planktonic cells. Detailed comparison also showed that cyclosporine seemed induce as compared tacrolimus.

It is widely known that Enterococcus faecalis virulence related to its biofilm formation. Although Enterococci are common commensal organisms of the gastrointestinal tract, difference between and pathogen strains remain unclear. In this study, we compare biochemical profile biofilms formed by two groups medical strains. The were isolated as pathogens from infections urinary tract other (wounds, pus bedsores), taken faeces healthy volunteers wild mallards (Anas platyrhynchos) living in an...

Infections caused by opportunistic pathogens such as enterococci remain difficult to manage, especially in immunocompromised patients. Because of infections' limited symptoms patients the additional problems are find proper diagnostic criteria and management infection. Here we aimed compare resistance commensal enterococcal strains RTx patients' isolates, PMNs phagocytosis. Thirty-six urine faecal isolates from 17 healthy volunteers were cultured planktonic biofilm forms 37°C or 42°C....

The Gram negative rods as Escherichia coli and Klebsiella pneumoniae belong to the most common etiology agents of urinary tract infections. aim our study was assess diversity biofilm formed in different diseases their impact on monocytes' adherence activation. bacteria were obtained from patients with kidney problems. Some after renal transplantation, some them not. Changes size granularity monocytes, well biofilm, assessed using FACSVerse flow cytometer 1 h co-incubation monocytes bacterial...

Infections caused by commensal bacteria may be fatal for the patients under immunosuppressive therapy. This results also from difficulty in identification of high risk strains. Enterococcal infections are increasingly frequent but despite many studies on virulence traits, difference between and pathogenic strains remains unclear. Prophages newly described as important elements competition during colonization, well pathogenicity Here we evaluate a presence pp4, pp1, pp7 prophages ASA...

The aim of our study was to assess the possibility predicting Enterococcus faecalis pathogenicity based on morphological changes monocytes interacting with bacteria and their adherence biofilm. Changes in size granularity monocytes, as well biofilm were assessed using FACScan flow cytometer after 1h co-incubation enterococcal 37°C. obtained results validated respect incubated without bacteria. most prominent observed case commensal interaction isolated from blood stream urine caused...

Adherence properties of 78 Enterococcus faecalis strains from clinical specimens and healthy volunteers were measured by two methods: microculture nephelometric assays. In addition selected genes involved in adherence pili formation detected PCR. They more prevalent compared with fecal isolates volunteers. Microculture assay showed good correlation between enterococcal the presence ebpA/ebpB genes. contrast to previous method, results not influenced Both methods together could provide...

Adoption of the property cytidine (cytosine-beta-d-riboside) deamination in staphylococci to distinguish Staphylococcus aureus from other staphylococci.A total 560 staphylococcal strains were examined. The test demonstrated a sensitivity 97.1% and specificity 98.8%. Of 249 S. (115 oxacillin-resistant) 58 coagulase-negative another 16 clumping factor-negative aureus. 74 deficient identified by 16S rRNA gene sequencing further investigated spa typing 13 types found.The deaminase (CDT) is...

Abstract Our study aimed to identify markers of enterococci’s virulence potential by evaluating the properties strains different sites isolation. Enterococcal were isolated as commensals from faeces and invasive urine blood patients University Clinical Centre, Gdańsk, Poland. Changes in monocytes’ susceptibility cytotoxic activity isolates origins their adherence biofilm evaluated using a flow cytometer. The bacterial protein profile was estimated matrix assisted laser desorption...

Adherence properties of 78 Enterococcus faecalis strains from clinical specimens and healthy volunteers were measured by two methods: microculture nephelometric assays. In addition selected genes involved in adherence pili formation detected PCR. They more prevalent compared with fecal isolates volunteers. Microculture assay showed good correlation between enterococcal the presence ebpA/ebpB genes. contrast to previous method, results not influenced Both methods together could provide...

Migration routes and migration strategies of Pipistrellus nathusii in Europe may suggest that there are several different populations this species. This pilot project aimed to check if genetic variability mitochondrial DNA could support hypothesis. Hair samples were taken from individuals Lithuania, Northern Poland The Netherlands. PCR amplifications mtDNA control region subsequent restriction analysis provided data computed using Arlequin Treecon softwares for AMOVA UPGMA clustering.....