A5025583812- Nanopore and Nanochannel Transport Studies
- Dark Matter and Cosmic Phenomena
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Analytical Chemistry and Sensors
- Particle physics theoretical and experimental studies
- Particle Detector Development and Performance
- Electrochemical Analysis and Applications
- Electrostatics and Colloid Interactions
- Atomic and Subatomic Physics Research
- Superconducting and THz Device Technology
- Bacteriophages and microbial interactions
- Radiation Detection and Scintillator Technologies
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- Microfluidic and Bio-sensing Technologies
- Pickering emulsions and particle stabilization
- Micro and Nano Robotics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Mechanical and Optical Resonators
- Ion-surface interactions and analysis
- Neutrino Physics Research
- Physics of Superconductivity and Magnetism
- Microfluidic and Capillary Electrophoresis Applications
- CCD and CMOS Imaging Sensors
Texas A&M University
2014-2025
Mitchell Institute
2020-2024
Loughborough University
2014-2023
University of Manchester
2002-2014
University College Dublin
2010-2013
Pennsylvania State University
2005-2007
University of Virginia
2003-2006
University of California, Berkeley
2003
University of Salford
2003
Children's National
1976-1980
We report a first search for weakly interacting massive particles (WIMPs) using the background rejection capabilities of SuperCDMS. An exposure 577 kg days was analyzed WIMPs with mass <30 GeV/c(2), signal region blinded. Eleven events were observed after unblinding. set an upper limit on spin-independent WIMP-nucleon cross section 1.2×10(-42) cm(2) at 8 GeV/c(2). This result is in tension WIMP interpretations recent experiments and probes new parameter space scattering masses <6
Extracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) tuneable resistive pulse sensing (TRPS), been reported to produce high variability particle count (EM) poor sensitivity detecting below 50 nm size (NTA TRPS), making accurate unbiased EV technically challenging. This study introduces direct stochastic optical reconstruction...
Abstract The performance of superconducting qubits is degraded by a poorly characterized set energy sources breaking the Cooper pairs responsible for superconductivity, creating condition often called “quasiparticle poisoning”. Both and low threshold dark matter calorimeters have observed excess bursts quasiparticles or phonons that decrease in rate with time. Here, we show silicon crystal glued to its holder exhibits low-energy phonon events more than two orders magnitude larger...
High-pressure liquid chromatography–tandem mass spectrometry was used to obtain a protein profile of Escherichia coli strain MG1655 grown in minimal medium with glycerol as the carbon source. By using cell lysate from only 3 × 10 8 cells, at least four different tryptic peptides were detected for each 404 proteins short 4-h experiment. At one peptide high reliability score 986 proteins. Because membrane underrepresented, second experiment performed preparation enriched membranes. An...
Mapping the landscape of possible macromolecular polymer sequences to their fitness in performing biological functions is a challenge across biosciences. A paradigm case aptamers, nucleic acids that can be selected bind particular target molecules. We have characterized sequence-fitness for aptamers binding allophycocyanin (APC) protein via novel Closed Loop Aptameric Directed Evolution (CLADE) approach. In contrast conventional SELEX methodology, selection and mutation aptamer was carried...
Abstract Resistive pulse sensing is used to monitor individual and aggregated rod‐shaped nanoparticles as they move through tunable pores in elastomeric membranes. By comparing particles of similar dimensions, it demonstrated that the resistive signal a rod fundamentally different from sphere. Rods can be distinguished using two measurements: blockade event magnitude (Δ i p ), which reveals particle's size, full width at half maximum (FWHM) duration, relates speed length. While observed Δ...
Resistive pulse sensors, RPS, are allowing the transport mechanism of molecules, proteins and even nanoparticles to be characterized as they traverse pores. Previous work using RPS has shown that size, concentration zeta potential analyte can measured. Here we use tunable resistive sensing (TRPS) which utilizes a pore monitor translocation times with DNA modified surfaces. We start by demonstrating particles used infer known standards then apply method measure change in particles. By...
Aptamer-modified nanomaterials provide a simple, yet powerful sensing platform when combined with resistive pulse technologies. Aptamers adopt more stable tertiary structure in the presence of target analyte, which results change charge density and velocity carrier particle. In practice is specific for each aptamer target, strength signal varies different applications experimental conditions. Resistive sensors (RPS) have single particle resolution, allowing detailed characterization sample....
The zeta potential of the protein corona around carboxyl particles has been measured using tunable resistive pulse sensing (TRPS). A simple and rapid assay for characterising potentials within buffer, serum plasma is presented monitoring change, magnitude distribution proteins on particle surface. First, we measure change in carboxyl-functionalised nanoparticles solutions that contain biologically relevant concentrations individual proteins, typically constituted serum, observe a significant...
A review and tutorial on tunable resistive pulse sensors used as biosensors.
Technologies that can detect and characterize particulates in liquids have applications health, food, environmental monitoring. Simply counting the numbers of cells or particles is not sufficient for most applications; other physical properties must also be measured. Typically, it necessary to compromise between speed a sensor its chemical biological specificity. Here, we present low-cost high-throughput multiuse counter classifies particle's size, concentration, shape. We report how...
We present the design and characterization of a cryogenic phonon-sensitive 1-gram Si detector exploiting Neganov-Trofimov-Luke effect to detect single-charge excitations. This device achieved 2.65(2)~eV phonon energy resolution when operated without voltage bias across crystal corresponding charge 0.03 electron-hole pairs at 100~V bias. With continuous-readout data acquisition system an offline optimum-filter trigger, we obtain 9.2~eV threshold with trigger rate order 20~Hz. The detector's...
Abstract Age‐related macular degeneration (AMD) is a leading cause of blindness. Vision loss caused by the retinal pigment epithelium (RPE) and photoreceptors atrophy and/or choroidal angiogenesis. Here we use AMD patient‐specific RPE cells with Complement Factor H Y402H high‐risk polymorphism to perform comprehensive analysis extracellular vesicles (EVs), their cargo role in disease pathology. We show that characterised enhanced polarised EV secretion. Multi‐omics analyses demonstrate EVs...
The SPICE/HeRALD collaboration is performing research and development to enable studies of sub-GeV dark matter models using a variety target materials. Here we report our recent progress on instrumenting superfluid He4 mass with transition-edge sensor based calorimeter detect both atomic signals (scintillation) quasiparticle (phonon roton) excitations. sensitivity HeRALD the critical "quantum evaporation" signal from quasiparticles requires us block film flow calorimeter. We have developed...
The conjugation of 14 nm diameter CoFe2O4 nanoparticles to the surface biotinylated microtubules enables their manipulation with externally applied magnetic fields small, permanent NdFeB magnets. Microtubules are selectively patterned on kinesin motor-modified glass surfaces in coparallel arrays that mimic orientation field lines over millimeter distances. is simultaneously used increase loading microtubules. We demonstrate motility across motor retained following functionalization...
Steering with magnets: Magnetic fields are used to control the direction of movement microtubules as they transported atop a kinesin-motor-covered surface. The heterogeneously labeled magnetic nanoparticles, thereby allowing leading end be directed in response field, while nonlabeled trailing maintains an unhindered interaction motor surface (see schematic representation). Supporting information for this article is available on WWW under...
Properties of biological fitness landscapes are interest to a wide sector the life sciences, from ecology genetics synthetic biology. For biomolecular landscapes, information we currently possess comes primarily two sources: sparse samples obtained directed evolution experiments; and more fine-grained but less authentic ‘ in silico ’ models (such as NK -landscapes). Here present entire protein-binding profile all variants nucleic acid oligomer 10 bases length, which have experimentally by...
Aptamers are short single-stranded pieces of DNA or RNA capable binding to analytes with specificity and high affinity. Due their comparable selectivity, stability, cost, over the last two decades, aptamers have started challenge antibodies in use on many technology platforms. The event often leads changes aptamer's secondary tertiary structure; monitoring such has led creation new analytical sensors. Here, we demonstrate a tunable resistive pulse sensing (TRPS) monitor interaction between...
A tunable resistive pulse sensor, utilising a polyurethane nanopore, has been used to characterise nanoparticles as they traverse the pore opening. Herein we demonstrate that translocation speed, conductive and magnitude, can be infer surface charge of nanoparticle, act specific transduction signal for binding metal ions ligands on particle surface. Surfaces silica were modified with ligand concept, extract copper(II) (Cu2+) from solution. By tuning pH ionic strength solution, biphasic...
The discovery and characterisation of nanomaterials represents a multidisciplinary problem. Their properties applications within biological, physical medicinal sciences depend on their size, shape, concentration surface charge. No single technology can currently measure all characteristics. Here we combine resistive pulse sensing with predictive logistic regression models, termed RPS-LRM, to rapidly characterise nanomaterial's aspect ratio, shape when mixtures nanorods nanospheres are...
We present the design and characterization of a large-area Cryogenic PhotoDetector designed for active particle identification in rare event searches, such as neutrinoless double beta decay dark matter experiments. The detector consists 45.6 cm2 surface area by 1-mm-thick 10.6 g Si wafer. It is instrumented with distributed network Quasiparticle-trap-assisted Electrothermal feedback Transition-edge sensors superconducting critical temperature Tc=41.5 mK to measure athermal phonons released...