A5115598440- Multicomponent Synthesis of Heterocycles
- Synthesis and biological activity
- Catalytic C–H Functionalization Methods
- Synthesis and Biological Evaluation
- Catalytic Cross-Coupling Reactions
- Synthesis and Biological Activity
- Sulfur-Based Synthesis Techniques
- Synthesis of Organic Compounds
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Reactions
- Synthesis and Catalytic Reactions
- Quinazolinone synthesis and applications
- Chemical synthesis and alkaloids
- Chemical Synthesis and Analysis
- Synthetic Organic Chemistry Methods
- Advanced Biosensing Techniques and Applications
- Marine Sponges and Natural Products
- Bioactive Compounds and Antitumor Agents
- Microwave-Assisted Synthesis and Applications
- Phenothiazines and Benzothiazines Synthesis and Activities
- Click Chemistry and Applications
- Cancer therapeutics and mechanisms
- Oxidative Organic Chemistry Reactions
- Advanced biosensing and bioanalysis techniques
- Asymmetric Hydrogenation and Catalysis
Vellore Institute of Technology University
2012-2024
Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
2018
Institute of Microbial Technology
2017
Korea Basic Science Institute
2014-2015
Protein Technologies (United Kingdom)
2005-2015
AstraZeneca (India)
2015
University of Manchester
2008-2013
Council of Scientific and Industrial Research
2013
Babraham Institute
2006-2008
University of Cambridge
2002-2004
Imbalance of signals that control cell survival and death results in pathologies, including cancer neurodegeneration. Two pathways are integral to setting the balance between controlled by lipid-activated protein kinase B (PKB)/Akt Ca(2+). PKB elicits its effects through phosphorylation inactivation proapoptotic factors. Ca(2+) stimulates many prodeath pathways, among which is mitochondrial permeability transition. We identified release inositol 1,4,5-trisphosphate receptor (InsP(3)R)...
There is a particular need in protein analysis and purification for specific, functional, generic methods of immobilization on solid supports. Here we describe double-hexahistidine (His6) tag sequence, comprising two hexahistidines separated by an 11-amino acid spacer, which shows at least 1 order magnitude stronger binding to Ni−NTA-modified surfaces than conventional single-His6 or tags N- C-termini. Using, as model, tagged versions green fluorescent (GFP), stable tight the double-His6...
We present an experimental and computational pipeline for the generation of kinetic models metabolism, demonstrate its application to glycolysis in Saccharomyces cerevisiae . Starting from approximate mathematical model, we employ a “cycle knowledge” strategy, identifying steps with most control over flux. Kinetic parameters individual isoenzymes within these are measured experimentally under standardised set conditions. Experimental strategies applied establish vivo concentrations...
Mapping the landscape of possible macromolecular polymer sequences to their fitness in performing biological functions is a challenge across biosciences. A paradigm case aptamers, nucleic acids that can be selected bind particular target molecules. We have characterized sequence-fitness for aptamers binding allophycocyanin (APC) protein via novel Closed Loop Aptameric Directed Evolution (CLADE) approach. In contrast conventional SELEX methodology, selection and mutation aptamer was carried...
A volatile organic solvent-free and choline chloride (ChCl)-based deep eutectic system (DES)-mediated sp3-CH functionalization of acetophenones 1 with benzyl alcohols 2 to the corresponding α, β-saturated ketones 3 is accounted for. The domino dehydrogenation–aldol condensation (hydrogenation borrowing concept) has been successfully attempted palladium-tetrakis(triphenylphosphine) [Pd(PPh3)4] catalyst–xantphos ligand combination. Furthermore, a sequential Friedländer reaction...
Antibody-based microarray is a novel technology with great promise in biomedicine that will provide unique means to perform global proteome analysis. In the process of designing high-density antibody microarrays required, several critical key issues have been identified remain be resolved. particular, there need for specific and selective approaches enabling non-purified probes directly purified, orientated coupled generic one-step procedure on chip. this study, we report successful design...
An efficient, solvent-free synthesis of 2-acylthieno[2,3-b]quinolines is reported from 2-halo-quinolinyl ketones through Cu-TEMPO catalyzed dehydrogenation, sp2-C–H functionalization using elemental sulfur as thiol surrogate (sulfur source) and tetrabutylammonium acetate an ionic reaction medium. The optimized conditions give excellent product yields under mild with chemoselectivity broad functional group tolerance. synthetic importance the synthesized molecules showcased further by...
Abstract An efficient nickel‐catalyzed sequential one‐pot strategy for the synthesis of coumarin‐appended quinolines and bis‐quinolines has been developed under mild reaction conditions in moderate to good yields (up 84 %) via dehydrogenative Friedlander annulation followed by C(sp 3 )−H functionalization. The use a cost‐effective Ni catalyst, environmentally friendly solvents simple operating procedures are attractive attributes this method. Additionally, photophysical properties...
The series of AB ring cores mappicine 5a-e were generated by the reaction 2-chloroquinoline-3-carbaldehyde 4a-e with NaBH 4 through eco-friendly route.All synthons characterized FTIR, 1 H-NMR, 13 C-NMR, LCMS and HRMS techniques.All synthesized compounds screened for their antioxidant activity, in vitro hemolytic on human erythrocytes cytotoxicity, HeLa Vero cells.Out all synthons, 5e exhibit good as well cytotoxicity activities, where 5d showed promising compound 5a...
The behaviour of biological systems can be deduced from their mathematical models. However, multiple sources data in diverse forms are required the construction a model order to define its components and biochemical reactions, corresponding parameters. Automating assembly use biology models is dependent upon integration processes involving interoperation analytical resources.Taverna workflows have been developed for automated quantitative parameterised metabolic networks Systems Biology...
Employing Burkhard Konig’s l-(+)-tartaric acid-dimethyl urea (DMU) mixture as an efficient catalyst or solvent for the Michael addition of various chalcones with N-arylhomophthalimides could successfully furnish corresponding 4-subtituted-N-arylhomophthalides under mild conditions. The melts were found to be inexpensive, nontoxic, simple use, high yielding, reusable, and biodegradable.
A series of spiro-[indoline-3,3′-pyrrolizin/pyrrolidin]-2-ones were synthesized from Cu–TEMPO catalyzed dehydrogenation followed by 1,3-dipolar cycloaddition azomethine ylides <italic>via</italic> decarboxylative condensation, resulting in high regioselectivities and yields.