A5074351912- Neuropeptides and Animal Physiology
- Genetic factors in colorectal cancer
- PI3K/AKT/mTOR signaling in cancer
- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Computational Drug Discovery Methods
- Digestive system and related health
- Colorectal Cancer Treatments and Studies
- Helicobacter pylori-related gastroenterology studies
- Cancer therapeutics and mechanisms
- Inflammatory mediators and NSAID effects
- Nanoparticle-Based Drug Delivery
- Pancreatic and Hepatic Oncology Research
- TGF-β signaling in diseases
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Infant Nutrition and Health
- Neuroendocrine Tumor Research Advances
- Mast cells and histamine
- Drug Transport and Resistance Mechanisms
- Cancer Immunotherapy and Biomarkers
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Wnt/β-catenin signaling in development and cancer
- Neuroendocrine regulation and behavior
Inserm
2012-2025
Sorbonne Université
1996-2025
Centre de Recherche Saint-Antoine
1986-2025
Hôpital Saint-Antoine
1993-2020
Assistance Publique – Hôpitaux de Paris
2020
Délégation Paris 7
2007-2018
Université Paris Cité
1998-2018
Centre de Recherche sur l'Inflammation
2016-2018
Centre National de la Recherche Scientifique
2016-2018
Sorbonne Paris Cité
2016-2018
Transforming growth factor β (TGF-β) is a multifunctional that induces wide variety of cellular processes which affect and differentiation. TGF-β exerts its effects through heteromeric complex between two transmembrane serine/threonine kinase receptors, the type I II receptors. However, intracellular signaling pathways receptors act to generate responses remain largely undefined. Here, we report initiates cascade leading stress-activated protein kinase/c-Jun N-terminal (SAPK/JNK) activation....
Imbalance of signals that control cell survival and death results in pathologies, including cancer neurodegeneration. Two pathways are integral to setting the balance between controlled by lipid-activated protein kinase B (PKB)/Akt Ca(2+). PKB elicits its effects through phosphorylation inactivation proapoptotic factors. Ca(2+) stimulates many prodeath pathways, among which is mitochondrial permeability transition. We identified release inositol 1,4,5-trisphosphate receptor (InsP(3)R)...
Leptin plays a key role regulating food intake, body weight and fat mass. These critical parameters are associated with an increased risk for digestive mammary gland cancer in the Western population. Here we determined whether leptin contributes to invasive phenotype of colonic kidney epithelial cells at various stages neoplastic progression. First, potently (EC 50 = 10–30 ng/ml) induces invasion collagen gels by premalignant familial adenomatous PC/AA/C1 nontumorigenic MDCK cells, their...
Abstract The interaction of neoplastic cells with the extracellular matrix is a critical event for initiation cancer invasion and metastasis. This study was designed to evaluate potential implication stromelysin‐3 (ST3), newly identified member matrix‐degrading metalloproteinase family, BM‐40/SPARC, glycoprotein associated matrix, during progression human colorectal cancers. We analyzed relative abundance ST3 BM‐40/SPARC transcripts by Northern blot, their distribution in situ hybridization,...
We recently established the critical role of lipid phosphatase activity PTEN tumor suppressor in stabilizing cell-cell contacts and suppressing invasiveness. To delineate effector systems involved, we investigated interaction with E-cadherin junctional complexes kidney colonic epithelial cell lines. p85 regulatory subunit phosphatidylinositol 3-OH kinase (PI3K) co-immunoprecipitated catenins. By using a yeast two-hybrid assay, demonstrated that interacted indirectly beta-catenin by binding...
Because the crucial role of angiogenesis has been demonstrated in tumor growth and metastasis, present study was undertaken to characterize relative expression vascular endothelial factors VEGF (vascular factor), VEGF-B, VEGF-C, their receptors KDR (kinase insert domain-containing receptor), FLT-1 (fms-like tyrosine kinase), FLT-4 human colonic cancers, relation Astler-Coller pathological classification, prognosis. VEGF-B gene quantified by Northern blot 72 samples matched with control...
Nanotechnology offers many possibilities to improve drug treatments, including with regard pharmacology. The current study reports a simple approach cisplatin efficacy in the treatment of colon cancer through creation orally administered squalenoylated nanoparticles loaded (SQ-CDDP NP). Cytotoxic effects SQ-CDDP NP were assessed human colonic cells and mouse models intestinal cancer. In cell culture, exhibited at least 10-fold greater cytotoxic potency compared uncomplexed cisplatin,...
Gastric M1 mucin and the MUC5AC gene show a similar oncofetal expression in colon. Our aim was to determine whether is product of gene. A recombinant baculovirus encoding C-terminal portion as fusion protein isolated immunoreactivity (rM) toward antibodies studied. Chicken also were raised against purified rM. Besides its reactivity with L56/C, serum recognizing bacterially expressed product, rM endowed immunoreactivity: (i) rM-expressing cells stained specifically anti-M1 monoclonal...
To analyze the implication of PTEN in control tumor cell invasiveness, canine kidney epithelial lines MDCKras-f and MDCKts-src, expressing activated Ras a temperature-sensitive v-Src tyrosine kinase, respectively, were transfected with expression vectors. Likewise, human PTEN-defective glioblastoma U87MG U373MG, melanoma line FM-45, prostate carcinoma PC-3 transfected. We demonstrate that ectopic wild-type MDCKts-src cells, but not mutants deficient either lipid or both protein phosphatase...
The search for effective chemopreventive compounds is a major challenge facing research into preventing the progression of cancer cells. naturally occurring polyphenol antioxidants look very promising, but their mechanism action still remains poorly understood. Here, we show that 2-(3,4-dihydroxyphenyl)ethanol (DPE), phenol antioxidant derived from olive oil, induces growth arrest and apoptosis in human colon carcinoma HT-29 mechanisms involve prolonged stress endoplasmic reticulum (ER)...
Resistin and resistin-like molecule (RELM)beta comprise a novel class of cysteine-rich proteins secreted into the circulation implicated in hepatic insulin resistance inflammation. RELMbeta is specifically produced by intestinal goblet cells but regulation its expression much local function are not elucidated. has been suggested to regulate colonic inflammation susceptibility, which dependent on mucosal barrier integrity.In this work we explored physiopathological role colon. Among agents...
PGE2 has been shown to induce relaxations in precontracted human pulmonary venous preparations, while arteries this response was not observed. We investigated and characterized the prostanoid receptors which are activated by veins.Human veins were cut as rings set up organ baths presence of a TP antagonist. A pharmacological study performed using selective EP1-4 ligands. The cellular localization EP4 immunohistochemistry their corresponding transcripts also these vessels.PGE2 agonists...
Distribution of transcripts for prolactin and growth hormone receptors (PRLR GHR) their isoforms was characterized in the gastrointestinal (GI) tract from several species by reverse transcription-polymerase chain reaction combined with Southern analysis. Human, rabbit, fetal adult rat PRLR GHR were detected isolated gastric glands, cell fractions, intestinal mucosa lineages. Human also observed throughout cancerous progression colonic adenomas to liver metastasis cancer lines at various...