A5081757413- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
- Osteoarthritis Treatment and Mechanisms
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- biodegradable polymer synthesis and properties
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Exercise and Physiological Responses
- Spectroscopy Techniques in Biomedical and Chemical Research
- Graphene and Nanomaterials Applications
- RNA Interference and Gene Delivery
- Dental Implant Techniques and Outcomes
- Facial Trauma and Fracture Management
- Genetic and Kidney Cyst Diseases
- Periodontal Regeneration and Treatments
- Nerve injury and regeneration
- Microfluidic and Bio-sensing Technologies
- Genetic Syndromes and Imprinting
- Electrohydrodynamics and Fluid Dynamics
University of Pavia
2020-2024
University of Nottingham
2015-2024
Policlinico San Matteo Fondazione
2023-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2023-2024
Versus Arthritis
2022
Universität für Weiterbildung Krems
2020
Indiana University School of Medicine
2015-2019
Park University
2012-2015
Queen's Medical Centre
2006-2011
Roslin Institute
2003-2008
Since their isolation in 1998, human embryonic stem (hES) cells have been shown to be capable of adopting various cell fates vitro. Here, we present vitro data demonstrating the directed commitment osteogenic lineage. Human ES are respond factors that promote osteogenesis, leading activation markers osteocalcin, parathyroid hormone receptor, bone sialoprotein, osteopontin, cbfa1, and collagen 1. Moreover, mineralized nodules obtained composed hydroxyapatite, further establishing similarity...
Human embryonic stem cells (hESCs) have the potential to generate multiple cell types and hold promise for future therapeutic applications. Although undifferentiated hESCs can proliferate indefinitely, hESC derivatives significantly downregulate telomerase limited replication potential. In this study we examine whether replicative lifespan of be extended by ectopic expression human reverse transcriptase (hTERT), catalytic component complex. To end, derived HEF1 cells, a fibroblast‐like type,...
Brown adipose tissue (BAT) is able to rapidly generate heat and metabolise macronutrients, such as glucose lipids, through activation of mitochondrial uncoupling protein 1 (UCP1). Diet can modulate UCP1 function but the capacity individual nutrients promote abundance activity not well established. Caffeine consumption has been associated with loss body weight increased energy expenditure, whether it activate unknown. This study examined effect caffeine on BAT thermogenesis in vitro vivo....
Extracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) tuneable resistive pulse sensing (TRPS), been reported to produce high variability particle count (EM) poor sensitivity detecting below 50 nm size (NTA TRPS), making accurate unbiased EV technically challenging. This study introduces direct stochastic optical reconstruction...
The manufacture of 3D scaffolds with specific controlled porous architecture, defined microstructure and an adjustable degradation profile was achieved using two-photon polymerization (TPP) a size 2 × 4 mm3. Scaffolds made from poly(D,L-lactide-co-ɛ-caprolactone) copolymer varying lactic acid (LA) ɛ -caprolactone (CL) ratios (LC16:4, 18:2 9:1) were generated via ring-opening-polymerization photoactivation. reactivity quantified photo-DSC, yielding double bond conversion ranging 70% to 90%....
An innovative approach was developed to engineer a multi-layered chitosan scaffold for osteochondral defect repair. A combination of freeze drying and porogen-leaching out methods produced porous, bioresorbable with distinct gradient pore size (mean = 160-275 μm). Incorporation 70 wt% nano-hydroxyapatite (nHA) provided additional strength the bone-like layer. The showed instantaneous mechanical recovery under compressive loading did not delaminate tensile loading. supported attachment...
Bone morphogenetic proteins (BMPs) were discovered as potent bone-inducing molecules. Their effect on adipogenic differentiation is not well understood, both stimulation and inhibition of the process have been described. We show here that BMP-2 strongly stimulates murine 3T3-L1 preadipocytes if applied together with an agonist peroxisome proliferator-activated receptor gamma (PPARgamma). On its own, (500 ng/ml) did stimulate adipogenesis quantified by flow cytometry lipophilic dye Nile Red....
5-Methylcytosine (5mC) is an epigenetic modification involved in regulation of gene activity during differentiation. Tet dioxygenases oxidize 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). Both 5fC 5caC can be excised from DNA by thymine-DNA glycosylase (TDG) followed regeneration unmodified cytosine via the base excision repair pathway. Despite evidence that this mechanism operative embryonic stem cells, role TDG-dependent demethylation...
Raman microspectroscopy (rms) was used to identify, image, and quantify potential molecular markers for label-free monitoring the differentiation status of live neural stem cells (NSCs) in vitro. Label-free noninvasive techniques characterization NCSs vitro are needed as they can be developed real-time cells. Principal component analysis (PCA) linear discriminant (LDA) models based on spectra undifferentiated NSCs NSC-derived glial enabled discrimination with 89.4% sensitivity 96.4%...
Breast cancer is a disease characterised by both genetic and epigenetic alterations. Epigenetic silencing of tumour suppressor genes an early event in breast carcinogenesis reversion gene reprogramming can provide clues to the mechanisms responsible for initiation progression. In this study we apply capacity oocytes cells order oncogenesis. We show that be directly reprogrammed amphibian oocyte extracts. The effect, after six hours treatment, absence DNA replication, includes demethylation...
Phosphate-based glasses (PBGs) are bioactive and fully degradable materials with tailorable degradation rates. PBGs can be produced as porous microspheres through a single-step process, using changes in their formulation geometry to produce varying pore sizes interconnectivity for use range of applications, including biomedical use. Calcium phosphate have recently been proposed orthobiologics, based on vitro cytocompatibility ion release profile. In this study, made two PBG formulations...
Low-power sonication is widely used to disaggregate extracellular vesicles (EVs) after isolation, however, the effects of on EV samples beyond dispersion are unclear. The present study analysed characteristics EVs collected from mesenchymal stem cells (MSCs) sonication, using a combination transmission electron microscopy, direct stochastic optical reconstruction and flow cytometry techniques. Results showed that intended disaggregation effect, lowest power setting available was enough alter...
Cells in the pluripotent ground state can give rise to somatic cells and germ cells, acquisition of pluripotency is dependent on expression Nanog. Pluripotency conserved primitive ectoderm embryos from mammals urodele amphibians, here we report isolation a Nanog ortholog axolotls (axNanog). axNanog does not contain tryptophan repeat domain expressed as monomer axolotl animal cap. The monomeric form sufficient regulate mouse embryonic stem but dimers are required rescue LIF-independent...
5-hydroxymethyl-cytosine (5-hmC) is a cytosine modification that relatively abundant in mammalian pre-implantation embryos and embryonic stem cells (ESC) derived from blastocysts. Recent observations imply both 5-hmC Tet1/2/3 proteins, catalyzing the conversion of 5-methyl-cytosine to 5-hmC, may play an important role self renewal differentiation ESCs. Here we assessed distribution zebrafish chick found that, unlike mammals, immunochemically undetectable these systems before onset...
Mesenchymal stem cells (MSCs) represent a valuable resource for regenerative medicine treatments orthopaedic repair and beyond. Following developments in isolation, expansion differentiation protocols, efforts to promote clinical translation of emerging cellular strategies now seek improve cell delivery targeting. This study shows efficient live MSC labelling using silica-coated magnetic particles (MPs), which enables 3D tracking guidance cells. A procedure developed the unassisted particle...
Brown and beige adipocytes are characterised as expressing the unique mitochondrial uncoupling protein (UCP)1 for which primary stimulus in vivo is cold exposure. The extent to cold-induced UCP1 activation can also be achieved vitro, therefore perform a comparable cellular function, unknown. We report an vitro model induce adipocyte browning using bone marrow (BM) derived mesenchymal stem cells (MSC), relies on differentiation at 32 °C instead of 37 °C. low temperature promoted adipogenic...
Although the use of embryonic stem cells in assisted repair musculoskeletal tissues holds promise, a direct comparison this cell source with adult marrow-derived has not been undertaken. Here we have compared osteogenic differentiation potential human (hESC) adult-derived vivo. hESC lines H7, H9, HEF-1 mesenchymal-like, telomerized H1 derivative, kidney epithelial line HEK293 (negative control), and mesenchymal (hMSC) were either used untreated or treated factors for 4 days prior to...
Stem cell therapy is widely acknowledged as a key medical technology of the 21st century which may provide treatments for many currently incurable diseases. These cells have an enormous potential replacement therapies to cure diseases such Parkinson's disease, diabetes and cardiovascular disorders, well in tissue engineering reliable source providing grafts replace repair diseased tissues. Nevertheless, progress this field has been difficult part because lack techniques that can measure...