ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTautomers of cytosine by microwave spectroscopyRonald D. Brown, Peter Godfrey, Donald McNaughton, and Anthony P. PierlotCite this: J. Am. Chem. Soc. 1989, 111, 6, 2308–2310Publication Date (Print):March 1, 1989Publication History Published online1 May 2002Published inissue 1 March 1989https://pubs.acs.org/doi/10.1021/ja00188a058https://doi.org/10.1021/ja00188a058research-articleACS PublicationsRequest reuse permissionsArticle...

ABSTRACT Fourier transform infrared (FTIR) spectroscopy in combination with the partial least squares (PLS) multivariative statistical technique was used for quantitative analysis of poly(β-hydroxybutyrate) (PHB) contents bacterial cells. A total 237 replicate spectra from 34 samples were obtained together gas chromatography-determined reference PHB contents. Using PLS regression, we able to relate contents, and correlation coefficient between measured predicted values optimal model a...

Several blood‐feeding organisms, including the malaria parasite detoxify haem released from host haemoglobin by conversion to insoluble crystalline ferriprotoporphyrin IX dimer known as haemozoin. To date mechanism of haemozoin formation has remained unknown, although lipids or proteins have been suggested catalyse its formation. We found that β‐haematin (synthetic haemozoin) forms rapidly under physiologically realistic conditions near octanol/water, pentanol/water and lipid/water...

Fourier-transform infrared (FTIR) microspectroscopy, combined with Principal Component Analysis (PCA), was applied in the study of exfoliated cervical cells from 272 patients. Six spectra were recorded for each patient, and these visually sorted into two types (type 1 type 2), based on their profiles. Spectra designated exhibited a profile characteristic normal epithelial cells, intense glycogen bands at 1022 cm−1 1150 cm−1, pronounced symmetric phosphate stretch 1078 cm−1. 2 features...

Resonance Raman spectra of β-hematin and hemin are reported for a range excitation wavelengths including 406, 488, 514, 568, 633, 780, 830, 1064 nm. Dramatic enhancement A1g modes (1570, 1371, 795, 677, 344 cm-1), ring breathing (850−650 out-of-plane iron−ligand (400−200 cm-1) were observed when irradiating with 780- 830-nm laser to lesser extent hemin. Absorbance recorded during the transformation showed red-shift Soret Q (0−1) bands, which has been interpreted as excitonic coupling...

We report the coupling of a portable Raman spectrometer to an acoustic levitation device enable environmental monitoring and potential taxonomic identification microalgae. Spectra living cells were recorded at 785 nm using fiber-optic probe coupled spectrometer. The spectra exhibit excellent signal-to-noise ratio clearly show bands from chlorophyll β-carotene. levitated photobleached microalgae reduction in concentration relative β-carotene after 10 min exposure quartz halogen lamp....

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMicrowave spectrum of uracilRonald D. Brown, Peter Godfrey, Donald. McNaughton, and Anthony P. PierlotCite this: J. Am. Chem. Soc. 1988, 110, 7, 2329–2330Publication Date (Print):March 1, 1988Publication History Published online1 May 2002Published inissue 1 March 1988https://pubs.acs.org/doi/10.1021/ja00215a069https://doi.org/10.1021/ja00215a069research-articleACS PublicationsRequest reuse permissionsArticle Views259Altmetric-Citations111LEARN...

Methods to probe the molecular structure of living cells are paramount importance in understanding drug interactions and environmental influences these complex dynamical systems. The coupling an acoustic levitation device with a micro-Raman spectrometer provides direct cellular chemistry containerless environment minimizing signal attenuation eliminating affects adhesion walls interfaces. We show that Raman spectroscopic (RALS) approach can be used monitor heme dynamics levitated 5 µL...

The interaction of nanoparticles with hemoglobin (Hb), a major constituent red blood cells, is important in nanotoxicity research. We report SERS spectra Hb using gold and silver at very small nanoparticle : molecule ratios, that is, under conditions relevant for SERS-based experiments cells high sensitivity. show the structural information obtained from experiment highly dependent on type substrate which molecules takes place. In isolated we demonstrate dependence used as extends to whole...

New diagnostic modalities for malaria must have high sensitivity and be affordable to the developing world. We report on a method rapidly detect quantify different stages of parasites, including ring gametocyte forms, using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT-IR) partial least-squares regression (PLS). The absolute detection limit was found 0.00001% parasitemia (<1 parasite/μL blood; p < 0.008) cultured early stage parasites in suspension normal...

An in vivo method for predicting the nutrient status of individual algal cells using Raman microspectroscopy is described. spectra 780 nm laser excitation show enhanced bands mainly attributable to chlorophyll a and beta-carotene. The relative intensities beta-carotene changed under nitrogen limitation, with becoming less intense more prominent. Although from N-replete N-starved cell populations varied, each distribution was distinct enough such that multivariate classification methods, as...

Hemoglobin nanocrystals were analyzed with tip-enhanced Raman scattering (TERS), surface-enhanced resonance (SERRS) and conventional (RRS) using 532 nm excitation. The extremely high spatial resolution of TERS enables selective enhancement heme, protein, amino acid bands from the crystal surface not observed in SERRS or RRS spectra. Two appearing at 1378 1355 cm(-1) assigned to ferric ferrous oxidation state marker bands, respectively, both spectra but spectrum bulk sample. results indicate...

Recently a resonant Mie scattering (RMieS) correction approach has been developed and demonstrated to be effective for removing the baseline distortions that compromise raw data in individual spectra. In this paper RMieS is extended FTIR images of tissue section from biopsy human cervical transformation zone coronal Wistar rat brain compared uncorrected images. It shown applying a) removes image spectra thus reveals previously hidden information on spatial variation chemical contents within...

Abstract Tip‐enhanced Raman scattering (TERS) spectroscopy is a promising technique for nanoscale chemical analysis. However, there are several challenges preventing widespread application of this technology, including reproducible fabrication efficient TERS probes. These problems reflect lack clear understanding the origins of, and parameters influencing TERS. It believed that coating characteristics at apex tip have major effect on near‐field optical enhancement thus activity metalized...

Mid-infrared spectra have been measured for cubic ice (Ic) nanoparticles (3–150 nm diameter) formed by rapid collisional cooling over a wide range of temperatures (5–209 K). Spectral diagnostics, such as the ratio surface related dangling OH to interior H-bonded stretch bands, reveal manner in which particle size depends on bath gas temperature and density, water molecule concentration. For particles smaller than 5 strained intermolecular bonds subsurface cause predominant peak position be...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMicrowave spectrum and unusual geometry of propadienone (methylene ketene)Ronald D. Brown, Peter Godfrey, Robert Champion, Donald McNaughtonCite this: J. Am. Chem. Soc. 1981, 103, 19, 5711–5715Publication Date (Print):September 1, 1981Publication History Published online1 May 2002Published inissue 1 September 1981https://pubs.acs.org/doi/10.1021/ja00409a016https://doi.org/10.1021/ja00409a016research-articleACS PublicationsRequest reuse...

The secondary structures of proteins (alpha-helical, beta-sheet, beta-turn, and random coil) in the solid state when bound to polymer beads, containing immobilized phenyl butyl ligands such as those commonly employed hydrophobic interaction chromatography, have been investigated using FTIR-ATR spectroscopy partial least squares (PLS) methods. Proteins with known structural features were used models, including 12 7 adsorbed onto surfaces. A strong PLS correlation was achieved between...