A5048022344- RNA modifications and cancer
- Advanced Electron Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- ZnO doping and properties
- Enzyme Structure and Function
- RNA Research and Splicing
- Clay minerals and soil interactions
- Catalytic Processes in Materials Science
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- Protein Structure and Dynamics
- Advanced X-ray Imaging Techniques
- Polyamine Metabolism and Applications
- Silicon Carbide Semiconductor Technologies
- Polymer Nanocomposites and Properties
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
- Semiconductor Quantum Structures and Devices
- MicroRNA in disease regulation
- Biochemical and Molecular Research
- Nanomaterials for catalytic reactions
- GaN-based semiconductor devices and materials
- RNA regulation and disease
- Extracellular vesicles in disease
- Mineralogy and Gemology Studies
Jagiellonian University
2022-2024
SOLARIS National Synchrotron Radiation Centre
2024
Maria Curie-Skłodowska University
2011-2018
Abstract The SOLARIS synchrotron located in Krakow, Poland, is a third-generation light source operating at medium electron energy. first was observed 2015, and the consequent development of infrastructure lead to users’ experiments soft X-ray energies 2018. Presently, expands its operation towards hard X-rays with continuous developments beamlines concurrent infrastructure. In following, we will summarize design, describe research together main performance parameters, upgrade, plans.
N6-methyladenosine (m6A) is an abundant, dynamic mRNA modification that regulates key steps of cellular metabolism. m6A in the coding regions inhibits translation elongation. Here, we show how modulates decoding bacterial system using a combination rapid kinetics, smFRET and single-particle cryo-EM. We that, while does not impair initial binding aminoacyl-tRNA to ribosome, presence fewer ribosomes complete process due lower stability complexes enhanced tRNA drop-off. The codon adopts...
Abstract Hypusination is a unique post-translational modification of the eukaryotic translation factor 5A (eIF5A) that essential for overcoming ribosome stalling at polyproline sequence stretches. The initial step hypusination, formation deoxyhypusine, catalyzed by deoxyhypusine synthase (DHS), however, molecular details DHS-mediated reaction remained elusive. Recently, patient-derived variants DHS and eIF5A have been linked to rare neurodevelopmental disorders. Here, we present cryo-EM...
Plants use solar energy to power cellular metabolism. The oxidation of plastoquinol and reduction plastocyanin by cytochrome b6f (Cyt b6f) is known as one the key steps photosynthesis, but catalytic mechanism in plastoquinone site (Qp) remains elusive. Here, we describe two high-resolution cryo-EM structures spinach Cyt homodimer with endogenous plastoquinones complex plastocyanin. Three are visible line up after another head tail near Qp both monomers, indicating existence a channel each...
Abstract Coronaviruses modify their single-stranded RNA genome with a methylated cap during replication to mimic the eukaryotic mRNAs. The capping process is initiated by several nonstructural proteins (nsp) encoded in viral genome. methylation performed two methyltransferases, nsp14 and nsp16, while nsp10 acts as co-factor both. Additionally, carries an exonuclease domain which operates proofreading system of Both nsp16 were reported independently bind nsp10, but available structural...
tRNA modifications affect ribosomal elongation speed and co-translational folding dynamics. The Elongator complex is responsible for introducing 5-carboxymethyl at wobble uridine bases (cm
Tn7 is a bacterial transposon with relatives containing element-encoded CRISPR-Cas systems mediating RNA-guided insertion. Here, we present the 2.7 Å cryoelectron microscopy structure of prototypic transposase TnsB interacting end DNA. When interacts across repeating binding sites, it adopts beads-on-a-string architecture, where DNA-binding and catalytic domains are arranged in tiled intertwined fashion. The form few base-specific contacts leading to preference that requires multiple weakly...
Purpose: This is a retrospective, observational case series of 43 patients who underwent Descemet’s striping automated endothelial keratoplasty with implants hydrophilic acrylic intraocular lenses hydrophobic surface (Lentis LS-302-Y or Lentis L-302-1). Methods: Patients diagnosed lens opacification after were identified from clinic records, minimal 18-month postoperative follow-up guaranteed in 36 eyes. Analysis included demographic data, indication for keratoplasty, ocular comorbidities,...
The proteasome activator PA200 binds to the catalytic core of proteasome, 20S, and activates its proteolytic activities. cellular function is poorly understood appears be cell type differentiation specific. Recent evidence suggests that not only standard 20S (s20S) but also specialized immunoproteasome (i20S) which plays a key role in anti-viral anti-tumor immunity. We here investigated interaction detail. show very first cryo-EM structures singly- doubly-capped i20S-PA200 complexes revealed...
Abstract Copper oxide nanoparticles were dispersed onto the surface of ceria nanorods, while a post‐synthesis modification was applied to reference sample using ammonia solutions. The modified catalysts characterized with variety analytical techniques, providing useful information on effect Cu:NH 3 ratio, employed in step, physicochemical properties. Re‐dispersion active copper species under extremely basic conditions promoted reducibility and oxygen mobility catalyst, thus resulting more...
Abstract Transfer RNA (tRNA) molecules are essential to decode messenger codons during protein synthesis. All known tRNAs heavily modified at multiple positions through post-transcriptional addition of chemical groups. Modifications in the tRNA anticodons directly influencing ribosome decoding and dynamics translation elongation crucial for maintaining proteome integrity. In eukaryotes, wobble uridines by Elongator, a large highly conserved macromolecular complex. Elongator consists two...
Abstract Betacoronaviruses are a genus within the Coronaviridae family of RNA viruses. They capable infecting vertebrates and causing epidemics as well global pandemics in humans. Mitigating threat posed by requires an understanding their molecular diversity. The development novel antivirals hinges on key regulatory elements viral genomes, particular 5′-proximal region, which is pivotal for protein synthesis. Using combination cryo-electron microscopy, atomic force chemical probing,...
The histidine biosynthetic pathway (HBP) is targeted for herbicide design with preliminary success only regarding imidazole-glycerol phosphate dehydratase (IGPD, EC 4.2.1.19), or HISN5, as referred to in plants. HISN5 catalyzes the sixth step of HBP, which (IGP) dehydrated imidazole-acetol phosphate. In this work, we present high-resolution cryoEM and crystal structures Medicago truncatula ( Mt HISN5) complexes an inactive IGP diastereoisomer various other ligands. can serve a new model...
Pseudouridine (Ψ), the isomer of uridine, is ubiquitously found in RNA, including tRNA, rRNA, and mRNA. Human pseudouridine synthase 3 (PUS3) catalyzes pseudouridylation position 38/39 tRNAs. However, molecular mechanisms by which it recognizes its RNA targets achieves site specificity remain elusive. Here, we determine single-particle cryo-EM structures PUS3 apo form bound to three tRNAs, showing how symmetric homodimer tRNAs positions target uridine next active site. Structure-guided...
Abstract Amyloid fibrils are fascinating and complex structures with the multilayered chiral organization. Using multimodal methodology, including VCD, ECD, cryo‐EM, TEM, we characterized in detail different levels of organization (secondary structure/protofilament/mesoscopic structure) amyloid prepared from proteins highly homologous structure (hen egg white human lysozymes). Our results demonstrate that small changes native protein or preparation conditions translate into significant...
Impact of Bi incorporation into (Ga,Mn)As layers on their electronic- and band-structures as well magnetic structural properties has been studied. Homogenous (Ga,Mn)(Bi,As) high perfection have grown by the low-temperature molecular-beam epitaxy technique. Post-growth annealing treatment results in an improvement increase hole concentration layers. The modulation photoreflectance spectroscopy are consistent with valence-band model hole-mediated ferromagnetism This material combines Ga(Bi,As)...
Recently, molecular approaches have been used to investigate the phylogeny of subtribe Oncidiinae, resulting in re-alignment several its genera. Here, a description is given structure floral elaiophores (oil glands) four species formerly assigned Oncidium Sw. Those Vitekorchis excavata (Lindl.) Romowicz & Szlach., Cyrtochilum meirax (Rchb.f.) Dalström and displaying dimorphism, namely O. heteranthum Poepp. Endl. var. album, are compared with that Gomesa longipes M.W. Chase N.H. Williams,...