A5007653477- Hedgehog Signaling Pathway Studies
- Cystic Fibrosis Research Advances
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Neonatal Respiratory Health Research
- Renal and related cancers
- Cancer and Skin Lesions
- Genetic and rare skin diseases.
- Tumors and Oncological Cases
- Microtubule and mitosis dynamics
- Developmental Biology and Gene Regulation
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- Chromatin Remodeling and Cancer
- Cancer Genomics and Diagnostics
- Advanced Breast Cancer Therapies
- Congenital Ear and Nasal Anomalies
- Protein Degradation and Inhibitors
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Tracheal and airway disorders
- Oral and Maxillofacial Pathology
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
The University of Queensland
2016-2025
Translational Research Institute
2020-2024
Queensland Children’s Hospital
2024
SUNY Upstate Medical University
2023
Research Institute for Bioscience and Biotechnology
2002-2013
University of Cologne
2013
ACT Government
2003
University of California, San Francisco
2003
The Ohio State University
2003
Albert Einstein College of Medicine
2003
Journal Article 'Touchdown' PCR to circumvent spurious priming during gene amplification Get access R.H. Don, Don Centre for Molecular Biology and Biotechnology, The University of QueenslandBrisbane Queensland 4072, Australia Search other works by this author on: Oxford Academic PubMed Google Scholar P. T. Cox, Cox * *To whom correspondence should be addressed B.J. Wainwright, Wainwright K. Baker, Baker J. S. Mattick Nucleic Acids Research, Volume 19, Issue 14, 25 July 1991, Page 4008,...
The nevoid basal cell carcinoma syndrome (NBCCS) is an autosomal dominant disorder characterized by multiple carcinomas (BCCs), pits of the palms and soles, jaw keratocysts, a variety other tumors, developmental abnormalities. NBCCS maps to chromosome 9q22.3. Familial sporadic BCCs display loss heterozygosity in this region, consistent with gene being tumor suppressor. A human sequence (PTC) strong homology Drosophila segment polarity gene, patched, was isolated from YAC cosmid contig...
Wnt genes have been implicated in a range of developmental processes the mouse including patterning central nervous system and limbs. Reported here for first time is expression Wnt2 early heart field 7.5-8.5 dpc (days post-coitum) embryos, making potentially useful gene marker stages development. Expression was also detected allantois from 8.0 at later placenta umbilicus. Mice deficient Wnt2, generated by targeting, displayed runting approximately 50% died perinatally. Histological analysis...
Drosophila patched is a segment polarity gene required for the correct patterning of larval segments and imaginal discs during fly development has close functional relationship with hedgehog. We have isolated complete human PATCHED cDNA sequence, which encodes putative protein 1296 amino acids, displays 39% identity 60% similarity to protein. Hydropathy analysis suggests that an integral membrane pattern hydrophobic hydrophilic stretches nearly identical patched. In developing mouse embryo,...
Abstract Mutations in the Hedgehog receptor, Patched 1 (Ptch1), have been linked to both familial and sporadic forms of basal cell carcinoma (BCC), leading hypothesis that loss Ptch1 function is sufficient for tumor progression. By combining conditional knockout technology with inducible activity Keratin6 promoter, we provide vivo evidence from population mouse skin induce rapid formation, reminiscent human BCC. Elimination does not promote nuclear translocation β-catenin ectopic activation...
The Sonic Hedgehog (Shh) pathway drives a subset of medulloblastomas, malignant neuroectodermal brain cancer, and other cancers. Small-molecule Shh inhibitors have induced tumor regression in mice patients with medulloblastoma; however, drug resistance rapidly emerges, some cases via de novo mutation the target. Here we assess response mechanisms to natural product derivative saridegib an aggressive Shh-driven mouse medulloblastoma model. In this model, treatment reduction significantly...
Medulloblastoma is curable in approximately 70% of patients. Over the past decade, progress improving survival using conventional therapies has stalled, resulting reduced quality life due to treatment-related side effects, which are a major concern survivors. The vast amount genomic and molecular data generated over last 5-10 years encourages optimism that improved risk stratification new targets will improve outcomes. It now clear medulloblastoma not single-disease entity, but instead...
Abstract Purpose: Bioinformatics analysis followed by in vivo studies patient-derived xenograft (PDX) models were used to identify and validate CDK 4/6 inhibition as an effective therapeutic strategy for medulloblastoma, particularly group 3 MYC-amplified tumors that have the worst clinical prognosis. Experimental Design: A protein interaction network derived from a Sleeping Beauty mutagenesis model of medulloblastoma was potential novel targets. The top hit this validated using PDX...
OLIG2-expressing tumor stem cells have been shown to drive recurrence in Sonic Hedgehog (SHH)-subgroup medulloblastoma (MB) and patients urgently need specific therapies target this cell population. Here, we investigate the therapeutic potential of brain-penetrant orally bioavailable, OLIG2 inhibitor CT-179, using SHH-MB explant organoids, PDX GEM models. We find that CT-179 disrupts dimerization, phosphorylation DNA binding alters cell-cycle kinetics, increasing differentiation apoptosis....
Susceptibility to infectious diseases is directed, in part, by the interaction between invading pathogen and host macrophages. This study examines influence of genetic background on host-pathogen interactions, assessing transcriptional responses macrophages from five inbred mouse strains lipopolysaccharide (LPS), a major determinant gram-negative microorganisms. The examined varied greatly number, amplitude rate induction genes expressed response LPS. was attenuated C3H/HeJlps d strain,...