A5071724940- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Lipid metabolism and biosynthesis
- Molecular Biology Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Interaction Studies and Fluorescence Analysis
- Geological and Geochemical Analysis
- Digital Imaging for Blood Diseases
- thermodynamics and calorimetric analyses
- Diet, Metabolism, and Disease
- Advancements in Transdermal Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Hematopoietic Stem Cell Transplantation
- Advanced Fluorescence Microscopy Techniques
- Planetary Science and Exploration
- Bee Products Chemical Analysis
- Advanced Biosensing Techniques and Applications
- Geology and Paleoclimatology Research
- 3D Printing in Biomedical Research
- Liver Disease Diagnosis and Treatment
- Climate Change and Health Impacts
- Pancreatic function and diabetes
- Optical Imaging and Spectroscopy Techniques
- Acute Lymphoblastic Leukemia research
Jagiellonian University
2015-2024
Krakow Cardiovascular Research Institute
2015-2019
Robert Koch Institute
2018
Faculty (United Kingdom)
2018
Abstract Endothelial HMEC-1 cells incubated with pro-inflammatory cytokine TNF-α for 6 and 24 hours were studied as a model of inflammation using Raman imaging. Striking changes in distribution, composition concentration cellular lipids observed after exposure to compared the control. In particular, 3D imaging revealed significant increase amount lipid entities formed under inflammation. Lipid bodies randomly distributed cytoplasm two types droplets assembled: more saturated one, spectral...
Identification of microorganisms by Fourier transform infrared (FT-IR) spectroscopy is known as a promising alternative to conventional identification techniques in clinical, food, and environmental microbiology. In this study we demonstrate the application FT-IR hyperspectral imaging for rapid, objective, cost-effective diagnosis pathogenic bacteria. The proposed method involves relatively short cultivation step under standardized conditions, transfer microbial material onto suitable IR...
In this work, 3D linear Raman spectroscopy was used to study lipid droplets (LDs) ex vivo in liver tissue and also vitro a single endothelial cell. Spectroscopic measurements combined with fluorescence microscopy and/or histochemical staining gave complex chemical information about LD composition enabled detailed investigations of the changes occurring various pathological states. Lipid analysis fatty performed using dietary mouse model steatosis, induced by high fat diet (HFD). HFD is...
Raman imaging was used to illustrate heterogeneity of a single endothelial cell and the vascular wall sample. The spectral analysis allowed for exploring complexity studied systems in three dimensions defining size, volume, shape biochemical composition cellular organelles. ability construct 3D maps by method that does not disrupt spatial integrity provided unique insight into architecture processes endothelium wall. may be considered as new trend analytical spectroscopy applied biomedical...
Human peripheral blood mononuclear cells (PBMCs) are a heterogeneous population of that includes T and B lymphocytes. The total number lymphocytes their percentage in the can be marker for diagnosis several human diseases. Currently, cytometric methods widely used to distinguish subtypes leukocytes quantify number. These techniques use cell immunophenotyping, which is limited by fluorochrome-labeled antibodies applied simultaneously. were isolated from obtained healthy donors. immunomagnetic...
In this work, confocal Raman imaging was used to study the formation of lipid droplets (LDs) in vitro a single endothelial cell upon incubation with polyunsaturated fatty acids (10 or 25 μM) including arachidonic acid (AA) and its deuterated analog (AA-d8), eicosapentaenoic (EPA) docosahexaenoic (DHA). Based on spectra obtained from cell, it possible investigate biochemical changes induced by addition acids. particular, content lipids formed LDs unsaturation degree were identified...
Chloroquine (CQ) is an antimalarial drug known to inhibit autophagy flux by impairing autophagosome–lysosome fusion. We hypothesized that altered CQ has a considerable influence on the lipid composition of endothelial cells. Thus, we investigated responses induced human microvascular cells (HMEC-1). HMEC-1 after exposure were measured using combined methodology based label-free Raman and fluorescence imaging. spectroscopy was applied characterize subtle chemical changes in contents their...
In this work the formation of lipid droplets (LDs) in human endothelial cells culture response to uptake polyunsaturated fatty acids (PUFAs) was studied. Additionally, an effect 1‐methylnicotinamide (MNA) on process LDs investigated. have been previously described structurally and some degree biochemically, however neither precise function nor factors responsible for LD induction clarified. Lipid droplets, sometimes referred literature as bodies are organelles known regulate neutrophil,...
Metabolism of endothelial cells (ECs) depends on the availability energy substrates. Since endothelium is first line defence against inflammation in cardiovascular system and its dysfunction can lead to development diseases, it important understand how glucose metabolism changes during inflammation. In this work, uptake was studied human microvascular (HMEC-1) high (HG), additionally an inflammatory state, using Raman imaging. HG state induced by incubation ECs with a deuterated analogue,...
Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) with the use of a slide-on germanium accessory followed by chemometric analysis allowed for providing meaningful information about biochemical composition single endothelial cell. In this work, methodology ATR-FTIR measurements dried cells and immersed in water solution is presented. The contact cell Ge crystal was set up manually monitored through integration amide I band. Additionally, imaging transreflection...
Anthracycline antibiotics display genotoxic activity towards cancer cells but their clinical utility is limited by cardiac and vascular toxicity.
B-cell precursor acute lymphoblastic leukemia (BCP-ALL) with chromosome translocations like
Chemotherapeutic anthracyclines, like doxorubicin (DOX), are drugs endowed with cytostatic activity and widely used in antitumor therapy. Their molecular mechanism of action involves the formation a stable anthracycline-DNA complex, which prevents cell division results death. It is known that elevated DOX concentrations induce DNA chain loops overlaps. Here, for first time, tip-enhanced Raman scattering was to identify localize intercalated isolated double-stranded calf thymus DNA,...