A5040956552- Nitric Oxide and Endothelin Effects
- Lipid metabolism and biosynthesis
- Spectroscopy Techniques in Biomedical and Chemical Research
- Hormonal Regulation and Hypertension
- Eicosanoids and Hypertension Pharmacology
- Ion Transport and Channel Regulation
- Vitamin K Research Studies
- Liver Disease Diagnosis and Treatment
- Drug-Induced Hepatotoxicity and Protection
- Metabolomics and Mass Spectrometry Studies
- Cholesterol and Lipid Metabolism
- Blood properties and coagulation
- Lipid metabolism and disorders
- Vitamin C and Antioxidants Research
- Adipose Tissue and Metabolism
- Cardiovascular Disease and Adiposity
- Cancer, Hypoxia, and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Neurological Disease Mechanisms and Treatments
- Cardiovascular Health and Disease Prevention
- Receptor Mechanisms and Signaling
- Cancer, Lipids, and Metabolism
- Angiogenesis and VEGF in Cancer
- Atherosclerosis and Cardiovascular Diseases
- Characterization and Applications of Magnetic Nanoparticles
Jagiellonian University
2016-2025
Yale University
2025
Background The impairment of endothelium‐dependent vasodilation, increased endothelial permeability, and glycocalyx degradation are all important pathophysiological components dysfunction. However, it is still not clear whether in atherosclerosis, injury precedes other features dysfunction or these events coincide. Methods Results Herein, we demonstrate that 4‐ to 8‐week‐old apolipoprotein E/low‐density lipoprotein receptor‐deficient mice, at the stage before development atherosclerotic...
Vascular smooth muscle cells (VSMC) are the most abundant cell type in artery’s media layer and regulate vascular tone lesion remodeling during atherogenesis. Like monocyte-derived macrophages, VSMCs accumulate excess lipids contribute to total intimal foam population human coronary plaques mouse aortic atheroma. While there extensive studies characterizing contribution of lipid metabolism macrophage immunometabolic responses atherosclerotic plaques, role VSMC regulating function vivo...
Mesenchymal transformation of pulmonary endothelial cells contributes to the formation a metastatic microenvironment, but it is not known whether this precedes or follows early metastasis formation. In present work, we characterize development nitric oxide (NO) deficiency and markers endothelial–mesenchymal transition (EndMT) in lung relation progression 4T1 breast cancer injected orthotopically mice. NO production, synthase (eNOS) phosphorylation status, EndMT lung, endothelium...
The role of Epithelial sodium channel (ENaC) activity in the regulation endothelial function is not clear. Here, we analyze ENaC endothelium-dependent vasodilation and permeability vivo mice with conditional αENaC subunit gene inactivation endothelium (endo-αENaC KO mice) using unique MRI-based analysis acetylcholine- flow-mediated dilation as well vascular permeability. Mice were challenged or Lipopolysaccharide (LPS, from Salmonella enterica serotype abortus equi, 10 mg/kg, i.p.). In...
Background Although advanced heart failure ( HF ) is a clinically documented risk factor for vascular cognitive impairment, the occurrence and pathomechanisms of impairment in early stages are equivocal. Here, we characterize development assess whether cerebral hypoperfusion or prothrombotic conditions involved. Methods Results Tgαq*44 mice with slowly developing isolated triggered by cardiomyocyte‐specific overexpression G‐αq*44 protein were studied before end‐stage , at ages 3, 6, 10...
Endothelial dysfunction (ED) and red blood cell distribution width (RDW) are both prognostic factors in heart failure (HF), but the relationship between them is not clear. In this study, we used a unique mouse model of chronic HF driven by cardiomyocyte-specific overexpression activated Gαq protein (Tgαq*44 mice) to characterize development peripheral ED occurrence structural nanomechanical biochemical changes cells (RBCs).Systemic was detected vivo 8-month-old Tgαq*44 mice, as evidenced...
Biogenesis of lipid droplets (LDs) in various cells plays an important role physiological and pathological processes. However, the function LDs endothelial physiology pathology is not well understood. In present work, we investigated formation prostacyclin (PGI2) generation vascular tissue isolated murine aortas following activation by proinflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA). The...
Abstract Adipose triglyceride lipase (ATGL) is involved in lipolysis and displays a detrimental pathophysiological role cardio-metabolic diseases. However, the organo-protective effects of ATGL-induced were also suggested. The aim this work was to characterize function lipid droplets (LDs) regulation endothelial function. ATGL-dependent LDs hydrolysis cytosolic phospholipase A 2 (cPLA )-derived eicosanoids production studied aorta, smooth muscle cells exposed exogenous oleic acid (OA) or...
Endothelial dysfunction is linked to impaired endothelial-dependent vasodilatation and permeability changes. Here, we quantify both of these phenomena associated with endothelial by MRI in vivo mice. function was evaluated the brachiocephalic artery (BCA) left carotid (LCA) ApoE/LDLR−/− high-fat diet (HFD)-fed mice as compared control (C57BL/6J). The 3D IntraGate® FLASH sequence used for evaluation changes vessels' cross-sectional area (CSA) volume following acetylcholine (Ach)...
One of the new targets untapped therapeutic potential is perivascular adipose tissue (pVAT). Based on Raman spectroscopy we demonstrated that lipid unsaturation degree was clearly distinct in various types tissues, particular thoracic and abdominal pVATs, influenced by age animals.
Endothelial dysfunction is recognized as a critical condition in the development of cardiovascular disorders. This multifactorial process involves changes biochemical and mechanical properties endothelial cells leading to disturbed release vasoprotective mediators. Hypercholesterolemia increased stiffness cortex are independently shown result reduced nitric oxide thus dysfunction. However, direct evidence linking these parameters each other missing. Here, novel method combining Raman...
The endothelium plays an important role in cancer metastasis, but the mechanisms involved are still not clear. In present work, we characterised changes endothelial function at early and late stages of breast progression orthotopic model murine mammary carcinoma (4T1 cells). Endothelial was analysed based on simultaneous microLC/MS-MRM quantification 12 endothelium-related biomarkers reflecting glycocalyx disruption [syndecan-1 (SDC-1), endocan (ESM-1)], inflammation [vascular cell adhesion...
Our objective was to investigate the effect of cholesterol [hypercholesterolemia and 7-ketocholesterol (7K)] on endoglin (Eng) expression regulation with respect endothelial or vascular dysfunction in vivo vitro. In experiments were performed 2-mo-old atherosclerosis-prone apolipoprotein E-deficient/LDL receptor-deficient (ApoE-/-/LDLR-/-) female mice their wild-type C57BL/6J littermates. vitro experiments, human aortic cells (HAECs) treated 7K. ApoE-/-/LDLR-/- developed hypercholesterolemia...
Activation of the coagulation cascade favours metastatic spread, but antithrombotic therapy might also have detrimental effects on cancer progression. In this study, we characterized dabigatran, a direct reversible thrombin inhibitor, pulmonary endothelial barrier and spread in murine model breast metastasis. Dabigatran etexilate (100 mg kg-1) was administered to mice twice daily by oral gavage. Pulmonary metastasis, endothelium permeability vivo, platelet reactivity were evaluated after...
The contribution of the shear stress-sensitive epithelial Na+ channel (ENaC) to mechanical properties endothelial cell surface under (patho)physiological conditions is unclear. This issue was addressed in vivo and vitro models for dysfunction. Cultured human umbilical vein cells (HUVEC) were exposed laminar (LSS) or non-laminar stress (NLSS). ENaC membrane insertion quantified using Quantum-dot-based immunofluorescence staining probed with Atomic Force Microscope (AFM) ex isolated aortae...
Endothelial cells line the lumen of all vessels in body and maintain vascular homeostasis. In particular, endothelial cell regeneration response to insult sustain functional layer. EdU (5-ethynyl-2'-deoxyuridine) is an alkyne-tagged proliferation probe that incorporates into newly synthesized DNA used for fluorescence imaging with use "click chemistry" reaction a fluorescent azide. Here, we utilized as click-free Raman tracking proliferation. was performed live cells, showing advantage over...
Phylloquinon (PK) and menaquinones (MK) are both naturally occurring compounds belonging to vitamin K group. Present study aimed comprehensively analyze the influence of PK in several models vascular dysfunction determine whether has vasoprotective properties, similar those previously described for MK. Effects MK on endothelial were studied ApoE/LDLR