A5084457606- Advanced NMR Techniques and Applications
- Advancements in Materials Engineering
- Electron Spin Resonance Studies
- Advanced MRI Techniques and Applications
- Solid-state spectroscopy and crystallography
- Advanced Welding Techniques Analysis
- Energetic Materials and Combustion
- Atomic and Subatomic Physics Research
- Intermetallics and Advanced Alloy Properties
- Cardiac Imaging and Diagnostics
- Hormonal Regulation and Hypertension
- Cardiovascular Disease and Adiposity
- Structural Engineering and Materials Analysis
- State Capitalism and Financial Governance
- Cardiovascular Function and Risk Factors
- Welding Techniques and Residual Stresses
- Central European Literary Studies
- Surface Treatment and Coatings
- Polish socio-economic development
- Mechanical and Thermal Properties Analysis
- Advanced Battery Materials and Technologies
- Flame retardant materials and properties
- High-Velocity Impact and Material Behavior
- Metal Alloys Wear and Properties
- Signaling Pathways in Disease
Jagiellonian University
2021-2025
Institute for Biomedical Engineering
2016-2024
ETH Zurich
2016-2024
Explomet (Poland)
2016-2022
University of Zurich
2020
Opole University of Technology
2016-2019
Maria Curie-Skłodowska University
2019
University of Zielona Góra
2018
Resonance Research (United States)
2017
Mayo Clinic
2017
Abstract Immune checkpoint inhibitors (ICIs) exhibit remarkable antitumor activity and immune-related cardiotoxicity of unknown pathomechanism. The aim the study was to investigate ICI class-dependent in vitro pembrolizumab’s (Pem’s) vivo, seeking for translational prevention means. Cytotoxicity investigated primary cardiomyocytes splenocytes, incubated with ipilimumab, Pem avelumab. Pem’s cross-reactivity assessed by circular dichroism (CD) on biotechnologically produced human murine PD-1...
Abstract Aim Chronic heart failure (CHF) is often linked to liver malfunction and systemic endothelial dysfunction. However, whether cardio‐hepatic interactions in involve dysfunction of sinusoidal cells (LSECs) not known. Here we characterize LSECs phenotype early end stages chronic a murine model. Methods Right ventricle (RV) function, features congestive hepatopathy, the primary were characterized Tgαq*44 mice, with cardiomyocyte‐specific overexpression Gαq protein, at age 4‐ 12‐month...
Protein disulfide isomerases (PDIs) are involved in many intracellular and extracellular processes, including cell adhesion cytoskeletal reorganisation, but their contribution to the regulation of fenestrations liver sinusoidal endothelial cells (LSECs) remains unknown. Given that supported on a cytoskeleton scaffold, this study aimed investigate whether major PDIs regulate fenestration dynamics primary mouse LSECs. PDIA3 PDIA1 were found be most abundant among PDI isoforms Taking advantage...
Lung vessel remodeling leads to increased pulmonary vascular resistance, causing arterial hypertension (PAH), and consequently right ventricular hypertrophy failure. In patients suffering from systemic sclerosis (SSc), PAH can occur is a life-threatening complication. Dysregulation of immune processes plays crucial role in remodeling, as has previously been shown Fos-related antigen-2 (Fra-2) transgenic (TG) mice, model SSc-PAH. Here, we investigate whether the Fra-2 TG driven by type 2...
Abstract Aims Tumour necrosis factor α (TNF-α) represents a classical pro-inflammatory cytokine, and its increased levels positively correlate with the severity of many cardiovascular diseases. Surprisingly, some heart failure patients receiving high doses anti-TNF-α antibodies showed serious health worsening. This work aimed to examine role TNF-α signalling on development progression myocarditis heart-specific autoimmunity. Methods results Mice genetic deletion (Tnf+/− Tnf−/−) littermate...
Abstract Hyperpolarized silicon particles have been shown to exhibit long spin-lattice relaxation times at room temperature, making them interesting as novel MRI probes. Demonstrations of hyperpolarized particle imaging focused on large micron size (average (APS) = 2.2 μm) they have, date, demonstrated much larger polarizations than nanoparticles. We show that also smaller silicon-29 (APS 55 ± 12 nm) can be with superior properties. A maximum polarization 12.6% in the solid state is reported...
A theory of dynamic nuclear polarisation (DNP) by thermal mixing is suggested based on purely quantum considerations. minimal 6-level microscopic model developed to test the and link it well-known thermodynamic model. Optimal conditions for polarization enhancement effects inhomogeneous broadening electron resonance are discussed. Macroscopic simulations spectra displaying good agreement with experiments, involving BDPA trityl free radicals, presented.
Several in vivo applications of dissolution dynamic nuclear polarization (DNP) require rapid successive injections hyperpolarized substrates. Here we present the design and performance a custom-built multisample DNP setup for small animal research.The consists commercial wide-bore magnet charged to 3.35 T, cryostat, 94-GHz microwave source, skeleton that accommodates four identical sample sticks. Each stick features dissolver locked into port lifter, which permits moving cup out liquid...
Purpose The purpose of this work was to study the contribution liver [1‐ 13 C]lactate lactate signal detected in heart following injection hyperpolarized C]pyruvate. Methods A slice‐selective saturation scheme incorporated into a hybrid metabolic imaging and spectroscopy approach selectively presaturate liver. Imaging C]pyruvate its downstream metabolites were sequentially interleaved same experiment with optional presaturation C]lactate. Six healthy rats measured, data acquired without...
Dissolution DNP has become an important method to generate highly polarized substrates such as pyruvic acid for in vivo imaging and localized spectroscopy. In a quest further increase the polarization levels, which is MRI employing 13C detection, we describe design implementation of new polarizer that suitable dissolution operation at 7 T static magnetic field temperature 1.4 K. We all sample preparation steps experimental details necessary optimize trityl based samples use our this higher...
The contribution of the shear stress-sensitive epithelial Na+ channel (ENaC) to mechanical properties endothelial cell surface under (patho)physiological conditions is unclear. This issue was addressed in vivo and vitro models for dysfunction. Cultured human umbilical vein cells (HUVEC) were exposed laminar (LSS) or non-laminar stress (NLSS). ENaC membrane insertion quantified using Quantum-dot-based immunofluorescence staining probed with Atomic Force Microscope (AFM) ex isolated aortae...
Abstract Age represents a major risk factor in heart failure (HF). However, the mechanisms linking ageing and HF are not clear. We aimed to identify functional, morphological transcriptomic changes that could be attributed cardiac model of slowly progressing Tgαq*44 mice reference process FVB mice. In mice, resulted impairment diastolic function basal coronary flow (CF), perivascular interstitial fibrosis without activity angiotensin-converting enzyme (ACE) or aldosterone plasma...
Micro- and nanoparticles of elemental, crystalline silicon represent an attractive target for a wide range applications spanning from quantum computing to contrast agents biomedical imaging applications. To overcome the low sensitivity 29Si nuclei in magnetic resonance, dynamic nuclear polarization (DNP), which exploits endogenous surface defects as source polarization, can be used temporarily boost spin bath. In present work, we have assessed number commercially available micro- concerning...
Relaxation plays a crucial role in the spin dynamics of dynamic nuclear polarisation. We review here two different strategies that have recently been used to incorporate relaxation models predict solid effect A detailed explanation is provided on how Lindblad–Kossakowski form master equation can be describe system. Fluctuations interactions with environment as cause are discussed and it demonstrated superoperator acting Liouville space density operator derived by averaging out non-secular...
Distributions of residual stress components in explosively welded three-layer plate composed titanium Grade 12, 1 and SA516 70 steel are investigated this paper. The study applied the sectioning hole-drilling strain gage methods for measurement. results have shown an inhomogeneous distribution, approximated by fourth-order polynomial along thickness a plate. Biaxial compressive state is observed 12 layers which change to tensile type vicinity interface between steel. Microstructure...
The current work focuses on the effect of time and temperature annealing change in structure cracks growth fatigue life steel-titanium bimetal obtained by explosive welding. Cyclic bending tests were performed for different levels specimens a rectangular cross-section. crack was measured microscopy. Structure changes steel, titanium bond area resulted micro-hardness distribution. relationship between level temperature, propagation cracks, is analyzed. heat treatment at 500 °C does not result...
Hyperglycemia linked to diabetes results in endothelial dysfunction. In the present work, we comprehensively characterized effects of short-term hyperglycemia induced by administration an insulin receptor antagonist, S961 peptide, on endothelium and perivascular adipose tissue (PVAT) mice. Endothelial function thoracic abdominal aorta 12-week-old male C57Bl/6Jrj mice treated for two weeks with infusion via osmotic pumps was assessed vivo using magnetic resonance imaging ex detection nitric...
Endothelial dysfunction is one of the hallmarks vascular abnormalities in metabolic diseases and has been repeatedly demonstrated coronary peripheral circulation mice fed high-fat diet (HFD), particularly after long-term HFD. However, temporal relationship between development microvascular endothelial deterioration diastolic systolic cardiac function short-term feeding with HFD not yet studied. This study aimed to correlate changes global performance indices vivo mice. Short-term a (60% fat...
This study sought to develop noninvasive, in vivo imaging schemes that allow for quantitative assessment of pulmonary microvascular functional status based on the combination T 1 mapping and dynamic contrast‐enhanced (DynCE) imaging. Ultrashort‐echo‐time (UTE) at 9.4 lung parenchyma was performed. Retrospective gating modulation first point each recorded spoke. maps were obtained using a series five consecutive images with varying RF angles analyzed variable flip angle approach. The mean...