A5003003528- Animal testing and alternatives
- Animal Behavior and Welfare Studies
- Silymarin and Mushroom Poisoning
- Drug-Induced Hepatotoxicity and Protection
- Nuts composition and effects
- RNA Interference and Gene Delivery
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes Treatment and Management
- Saffron Plant Research Studies
- Diet and metabolism studies
- Protease and Inhibitor Mechanisms
- Olfactory and Sensory Function Studies
- Human-Animal Interaction Studies
- Metabolism, Diabetes, and Cancer
- Adipose Tissue and Metabolism
- Cardiac Fibrosis and Remodeling
- Cardiac Valve Diseases and Treatments
- Liver Disease Diagnosis and Treatment
- Cardiac Ischemia and Reperfusion
- Pancreatic function and diabetes
- Lipoproteins and Cardiovascular Health
- Wireless Body Area Networks
- Veterinary Pharmacology and Anesthesia
- Fatty Acid Research and Health
- Antiplatelet Therapy and Cardiovascular Diseases
Academy of Athens
2016-2025
Biomedical Research Foundation of the Academy of Athens
2016-2025
National and Kapodistrian University of Athens
2003-2018
Christ University
2018
Foundation for Biomedical Research
2007-2017
Athens Medical Center
1998
Empagliflozin (EMPA), a drug approved for type 2 diabetes management, reduced cardiovascular death but is unknown if it reduces myocardial infarction. We sought to investigate: (i) the effect of EMPA on function and infarct size after ischemia/reperfusion in mice fed with western diet (WD), (ii) underlying signaling pathways, (iii) its effects cell survival rat embryonic-heart-derived cardiomyoblasts (H9C2) endothelial cells (ECs). To facilitate aforementioned aims, were initially randomized...
Hepatocellular carcinoma (HCC) occurs predominantly in patients with liver cirrhosis. Here we show an innovative RNA-based targeted approach to enhance endogenous albumin production while reducing tumor burden. We designed short-activating RNAs (saRNA) expression of C/EBPα (CCAAT/enhancer-binding protein-α), a transcriptional regulator and activator gene expression. Increased levels both mRNA addition 3-fold increase secretion 50% decrease cell proliferation was observed C/EBPα-saRNA...
Abstract Immune checkpoint inhibitors (ICIs) exhibit remarkable antitumor activity and immune-related cardiotoxicity of unknown pathomechanism. The aim the study was to investigate ICI class-dependent in vitro pembrolizumab’s (Pem’s) vivo, seeking for translational prevention means. Cytotoxicity investigated primary cardiomyocytes splenocytes, incubated with ipilimumab, Pem avelumab. Pem’s cross-reactivity assessed by circular dichroism (CD) on biotechnologically produced human murine PD-1...
Although collagen membranes have been clinically applied for guided tissue/bone regeneration more than 30 years, their in vivo degradation pattern has never fully clarified. A better understanding of the different stages is extremely important, considering that biology bone requires presence a stable and cell/tissue-occlusive barrier during healing order to ensure predictable result. Therefore, aim this study was investigate porcine non-cross-linked membrane an model (GBR).Decalcified...
Aims: Empagliflozin (EMPA) demonstrates cardioprotective effects on diabetic myocardium but its infarct-sparing in normoglycemia remain unspecified. We investigated the acute and chronic effect of EMPA infarct size after ischemia-reperfusion (I/R) injury mechanisms cardioprotection nondiabetic mice. Results: Chronic oral administration (6 weeks) reduced myocardial 30 min/2 h I/R (26.5% ± 3.9% vs 45.8% 3.3% control group, p < 0.01). Body weight, blood pressure, glucose levels, cardiac...
Gut microbiota holds a key-role in numerous biological functions and has emerged as driving force for the development of diabetes. Diet contributes to gut diversity functionality providing tool prevention management disease. The study aimed investigate effect dietary intervention with pistachio nuts, rich source monounsaturated fatty acids, fibers phytochemicals on composition rat model Type 1 Diabetes.Male Wistar rats were randomly assigned into four groups: healthy animals which received...
Abstract Recent research findings have established a close relationship between gut microbiota and atherosclerosis development; hence, focus has shifted towards modifying through probiotics administration. We thereby investigated the impact of Limosilactobacillus fermentum ACA-DC 179 on progression in apolipoprotein E-deficient (ApoE −/− ) mice. Twelve-week-old ApoE male female mice were treated with low (10 6 CFU/mouse) or high 9 dose L. daily for 8 weeks. Microbiota faeces during...
Physical exercise is the cornerstone of cardiovascular disease treatment. The present study investigated whether training affects atherosclerotic plaque composition through modification inflammatory-related pathways in apolipoprotein E knockout (apoE(-/-)) mice with diabetic atherosclerosis. Forty-five male apoE(-/-) were randomized into three equivalent (n=15) groups: control (CO), sedentary (SED), and (EX). Diabetes was induced by streptozotocin administration. High-fat diet administered...
Levosimendan (LEVO) a clinically-used inodilator, exerts multifaceted cardioprotective effects. Case-studies indicate protection against doxorubicin (DXR)-induced cardiotoxicity, but this effect remains obscure. We investigated the and mechanism of different regimens levosimendan on sub-chronic chronic cardiotoxicity.Based preliminary in vivo experiments, rats serving as model doxorubicin-cardiotoxicity were divided into: Control (N/S-0.9%), DXR (18 mg/kg-cumulative), DXR+LEVO (LEVO, 24...
Clinical studies have previously established the role of olive products in cardiovascular disease (CVD) prevention, whilst identification responsible constituents for beneficial effects is still pending. We sought to assess and compare cardioprotective potential oleuropein (OL), hydroxytyrosol (HT), oleocanthal (OC) oleanolic Acid (OA), regarding Ischemia/Reperfusion Injury (IRI) CVD risk factors alleviation. The scope study was design a potent safe combinatorial therapy...
<i>Aim:</i> To investigate the effects of exercise on atherosclerotic plaque composition, concentration matrix metalloproteinases (MMPs) in and systemic circulation. <i>Methods:</i> Ninety apolipoprotein E-deficient (apoE<sup>–/–</sup>) mice (45 male) were randomized to following groups (n = 15 each): control male/female; sedentary male/female. Mice kept a 16-week high-fat diet. Subsequently, sacrificed, while rest animals placed normal diet for 6 more...
This study was designed to investigate the transient and cumulative impairments in spatial non-spatial memory of C57Bl/6J mice exposed GSM 1.8 GHz signal for 90 min daily by a typical cellular (mobile) phone at specific absorption rate value 0.11 W/kg. Free-moving male 2 months old were irradiated two experimental protocols, lasting 66 148 days respectively. Each protocol used three groups animals (n = 8 each exposed, sham controls) combination with behavioural paradigms, object recognition...
Urinary bladder cancer is one of the most fatal and expensive diseases industrialized world. Despite strenuous efforts, no seminal advances have been achieved for its clinical management. Given importance metabolic reprogramming in cell survival growth, we herein employed 3-BrPA, a halogenated derivative pyruvate historically considered inhibitor glycolysis, to eliminate cells with highly oncogenic molecular signatures.Bladder were exposed 3-BrPA absence or presence several specific...
Abstract Objectives To histologically define the healing events occurring in calvarial critical size defects ( CSD s) following treatment with a collagen barrier for guided bone regeneration GBR ) and particulate graft healthy osteoporotic conditions. Material Methods Thirty‐six 10‐month‐old, female, Wistar rats were used this study. Half of them ovariectomized OVX fed low‐calcium diet to induce an osteoporotic‐like status. In each animal both groups, two 5‐mm s created, one centre parietal...
Although accumulation of lymphocytes in the white adipose tissue (WAT) obesity is linked to insulin resistance, it remains unclear whether also participate regulation energy homeostasis WAT. Here, we demonstrate enhanced dissipation Rag1-/- mice, increased catecholaminergic input subcutaneous WAT, and significant beige adipogenesis. Adoptive transfer experiments demonstrated that CD8+ T cell deficiency accounts for adipogenesis mice. Consistently, identified CD8-/- mice presented with The...
Glycogen synthase kinase 3 beta (GSK3β) link with the mitochondrial Permeability Transition Pore (mPTP) in cardioprotection is debated. We investigated role of GSK3β ischaemia (I)/reperfusion (R) injury using pharmacological tools.Infarct size inhibitor BIO (6-bromoindirubin-3'-oxime) and several novel analogues (MLS2776-MLS2779) was determined anaesthetized rabbits mice. In myocardial tissue inhibition specificity compounds tested. The mechanism protection focused on autophagy-related...