A5052850985- Multiple Myeloma Research and Treatments
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Nanoplatforms for cancer theranostics
- PARP inhibition in cancer therapy
- Histone Deacetylase Inhibitors Research
- Toxin Mechanisms and Immunotoxins
- Genomics and Chromatin Dynamics
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Chemotherapy-induced cardiotoxicity and mitigation
- DNA Repair Mechanisms
- Advancements in Transdermal Drug Delivery
- Wnt/β-catenin signaling in development and cancer
- Protein Degradation and Inhibitors
- Cancer Genomics and Diagnostics
- Diabetes Treatment and Management
- Hedgehog Signaling Pathway Studies
- Paraoxonase enzyme and polymorphisms
- Advanced Breast Cancer Therapies
- Receptor Mechanisms and Signaling
- Renin-Angiotensin System Studies
- CRISPR and Genetic Engineering
- Alkaline Phosphatase Research Studies
- Laser Material Processing Techniques
Academy of Athens
2015-2025
Biomedical Research Foundation of the Academy of Athens
2016-2025
Foundation Center
2024
The urothelium is a specialized epithelium that lines the urinary tract. It consists of three different cell types, namely, basal, intermediate and superficial cells arranged in relatively distinct layers. Normally, quiescent, it regenerates fast upon injury, but regeneration process not fully understood. Although several reports have indicated existence progenitors, their identity exact topology, as well role key processes such tissue carcinogenesis been clarified. Here we show minor...
Article21 January 2019Open Access Source DataTransparent process The lysine-specific methyltransferase KMT2C/MLL3 regulates DNA repair components in cancer Theodoros Rampias orcid.org/0000-0002-5460-5334 Biomedical Research Foundation Academy of Athens, Greece Search for more papers by this author Dimitris Karagiannis orcid.org/0000-0002-4394-1076 Margaritis Avgeris orcid.org/0000-0002-2135-9886 Department Biochemistry and Molecular Biology, Faculty National Kapodistrian University Alexander...
Abstract Therapeutic targeting of tumor cells with drug nanocarriers relies upon successful interaction membranes and efficient cell internalization. A further consideration is that engineered nanomaterials should not damage healthy tissues contact. critical factor in this process the external coating delivery nanodevices. Using silico, vitro vivo studies, we show for first time magnetic nanoparticles coated polyarabic acid have superior imaging, therapeutic, biocompatibility properties. We...
Aims: Empagliflozin (EMPA) demonstrates cardioprotective effects on diabetic myocardium but its infarct-sparing in normoglycemia remain unspecified. We investigated the acute and chronic effect of EMPA infarct size after ischemia-reperfusion (I/R) injury mechanisms cardioprotection nondiabetic mice. Results: Chronic oral administration (6 weeks) reduced myocardial 30 min/2 h I/R (26.5% ± 3.9% vs 45.8% 3.3% control group, p < 0.01). Body weight, blood pressure, glucose levels, cardiac...
Head and neck squamous cell carcinoma (HNSCC) is characterized by a high rate of locoregional or distant relapse among patients. It well established that resistance to chemotherapeutic drugs has an important role in the emergence recurrent and/or metastatic type this malignancy which associated with poor prognosis. Therefore, understanding molecular basis chemoresistance head cancer required for development effective therapeutic strategies. Activating mutations HRAS gene are driver events...
Abstract Due to its highly heterogenous molecular landscape, bladder cancer (BlCa) is still characterized by non-personalized prognosis and treatment decisions. N6-methyladenosine (m6A) has emerged as the most common conserved internal mRNA modification, regulating RNA metabolism translation. Herein, we have profiled mutations copy number variations (CNVs) within m6A machinery genes assessed their clinical relevance in BlCa patients’ outcome. DNA-seq libraries were prepared from 96 specimens...
Cancer treatment with chemotherapeutic drugs remains to be challenging the physician due limitations associated lack of efficacy or high toxicities. Typically, are administered intravenously, leading drug concentrations that drive but also lead known side effects. Delivery through transdermal microneedles (MNs) has become an important alternative approach. Such delivery options well suited for in which sustained levels would desirable. In context developing a novel approach, laser-induced...
Glioblastoma (GBM) is an aggressive malignant primary brain tumor with limited therapeutic options. We show that the angiotensin II (AngII) type 2 receptor (AT R) a target for GBM and AngII, endogenously produced in cells, promotes proliferation through AT R. repurposed EMA401, R antagonist originally developed as peripherally restricted analgesic, showed it inhibits of R-expressing spheroids blocks their invasiveness angiogenic capacity. The crystal structure bound to EMA401 was determined...
Abstract The kinase haspin phosphorylates histone H3 at threonine-3 (H3T3ph) during mitosis. H3T3ph provides a docking site for the Chromosomal Passenger Complex centromere, enabling correction of erratic microtubule-chromosome contacts. Although this mechanism is operational in all dividing cells, haspin-null mice do not exhibit developmental anomalies, apart from aberrant testis architecture. Investigating problem, we show here that mouse embryonic stem cells lack or overexpress haspin,...
Bladder cancer (BlCa) is an extensively heterogeneous disease that leads to great variability in tumor evolution scenarios and lifelong patient surveillance, emphasizing the need for modern, minimally invasive precision medicine. Here, we explored clinical significance of copy number alterations (CNAs) BlCa. CNA profiling was performed 15 patient-derived xenografts (PDXs) validated The Cancer Genome Atlas BlCa (TCGA-BLCA; n = 408) Lindgren et al. (n 143) cohorts. CDKN2A loss identified as...
The multiplexity of cancer has rendered it the second leading cause mortality worldwide and theragnostic prodrugs have gained popularity in recent years as a means treatment. Theragnostic enable simultaneous diagnosis therapy tumors via high-precision real-time drug release monitoring. Herein, we report development small prodrug GF, based on nucleoside anticancer agent gemcitabine fluorescent dye 5(6)-carboxyfluorescein. We successfully demonstrated its efficient internalization tumor cells,...
Recent advances in sequencing technologies have allowed the in-depth molecular study of tumors, even at single cell level. Sequencing efforts uncovered a previously unappreciated heterogeneity among tumor cells, which has been postulated to be driving force evolution and facilitate recurrence, metastasis, drug resistance. In current study, focused on early-stage operable non-small lung cancer, we used growth patient-derived xenograft (PDX) models mice as fast-forward process investigate...
Small molecule inhibitors targeting specific proteins are claiming a continuously growing share in cancer therapy, more commonly combination with traditional chemotherapeutic drugs. While these taken orally, the majority of chemotherapies administered through intravenous injection hospital premises. Alternative routes for chemotherapy administration would allow frequent at lower dosing by patient oneself, allowing treatment reduced side effects. Here, we employed laser printing to prepare...
Elevated levels of alkaline phosphatase (ALP) in the tumor microenvironment (TME) are a hallmark cancer progression and thus inhibition ALP could serve as an effective approach against cancer. Herein, we developed novel prodrug to tackle that bears self-inhibiting phosphatase-responsiveness properties can enhance at same time solubility parent compound. To probe this concept, selected apigenin cytotoxic agent since first unveiled, it directly interacts inhibits activity. Consequently,...
The Notch signaling pathway has been implicated in homeostasis and disease, including cancer, various tissues. Moreover, it involved both stem cell maintenance differentiation, a context-dependent manner. Stem/progenitor cells, on the other hand, have long suspected to be cells of origin malignancies. In order gain insight role ligand Dll1 mouse development, we generated knock-in line expressing an inducible Cre recombinase. We employed vivo approaches mice genetically mark rare...
Worldwide, there is a great gap between the demand and supply of organs for transplantations. Organs generated from patients' cells would not only solve problem transplant availability but also overcome complication incompatibility tissue rejection by host immune system. One most promising methods tested production in vivo blastocyst complementation (BC). Regrettably, BC suitable creation hearts. We have developed novel method, induced (iBC), to surpass this shortcoming. By applying iBC, we...
Abstract Background/Introduction We have recently shown that empagliflozin (EMPA), a sodium-glucose cotransporter 2 (SGLT2) inhibitor approved for type diabetes mellitus management reduces myocardial infarct size in diabetic mice undergone ischemia/reperfusion (I/R) after chronic administration. However its effect on non-diabetic myocardium remains unspecified. Purpose aimed to investigate: (i) the of EMPA function and I/R healthy mice, absence mellitus, (ii) underlying signaling pathways,...
The use of different 3D printing technologies for pharmaceutical manufacturing provides new opportunities personalized medicine with adjusted doses in various shapes and structures. In this work, we present a novel method dosage forms onto edible wafer papers as orodispersible thin films, targeting diseases such types cancer. Orodispersible films (ODFs) hold promise drug delivery they are easy to administer young older patients, bypass absorption by the digestive system minimize risk partial...