A5029937461- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Liver Diseases and Immunity
- Renal Transplantation Outcomes and Treatments
- Cutaneous Melanoma Detection and Management
- Organ Transplantation Techniques and Outcomes
- Adipose Tissue and Metabolism
- Cytomegalovirus and herpesvirus research
- melanin and skin pigmentation
- Adipokines, Inflammation, and Metabolic Diseases
- Skin Protection and Aging
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- Extracellular vesicles in disease
- Liver Disease Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- Trace Elements in Health
- Regulation of Appetite and Obesity
- Psoriasis: Treatment and Pathogenesis
- Diet, Metabolism, and Disease
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Iron Metabolism and Disorders
- Machine Learning in Materials Science
- Functional Brain Connectivity Studies
King's College London
2015-2024
University College London
2015-2024
King's College Hospital
2015-2024
University of London
2015-2024
King's College Hospital NHS Foundation Trust
2023
Medical Research Council
2015-2022
Biomedical Research Foundation of the Academy of Athens
2011-2018
Academy of Athens
2009-2018
University of Crete
2018
National and Kapodistrian University of Athens
2010-2015
Regulatory T cells (Tregs) are a lymphocyte subset with intrinsic immunosuppressive properties that can be expanded in large numbers ex vivo and have been shown to prevent allograft rejection promote tolerance animal models. To investigate the safety, applicability, biological activity of autologous Treg adoptive transfer humans, we conducted an open-label, dose-escalation, Phase I clinical trial liver transplantation. Patients were enrolled while awaiting transplantation or 6-12 months...
Significance Preservation and/or enhancement of Treg function is becoming a key component modern immunotherapeutic strategies, but the direct influence many immunosuppressive drugs on Tregs remains unknown. Calcineurin inhibitors (CNIs), which are widely used to treat inflammatory disorders, reduce size pool substantially, and this reduction might hinder their overall beneficial effects. Here we show that decrease in numbers caused by increased cell death as result limited availability IL-2...
CD4
Lim, Tiong Y.; Martinez‐Llordella, Marc; Kodela, Elisavet; Gray, Elisabeth; Heneghan, Michael A.; Sanchez‐Fueyo, Alberto , M.D., Ph.D. Author Information
Management of long-term immunosuppression following liver transplantation (LT) remains empirical. Surveillance biopsies in combination with transcriptional profiling could overcome this challenge by identifying recipients active alloimmune-mediated damage despite normal tests, but approach lacks applicability. Our aim was to investigate the utility non-invasive tools for stratification stable survivors LT, according their immunological risk and need immunosuppression.We conducted a...
Hepatic expression of iron homeostasis genes and serum parameters predict the success immunosuppression withdrawal following clinical liver transplantation, a phenomenon known as spontaneous operational tolerance. In experimental animal models, allograft tolerance is established through process that requires intra-hepatic lymphocyte activation deletion. Our aim was to determine if changes in systemic status regulate responses. We used murine model lymphocyte-mediated acute inflammation...
In several murine models of transplantation, the "cross-dressing" recipient antigen presenting cells (APCs) with intact donor major histocompatibility complex (MHC) derived from allograft-released small extracellular vesicles (sEVs) has been recently described as a key mechanism in eliciting and sustaining alloimmune responses. Investigation these processes clinical organ transplantation has, however, hampered by lack sensitivity conventional instruments assays. We have employed advanced...
Background Genetic association studies have revealed numerous polymorphisms conferring susceptibility to melanoma. We aimed replicate previously discovered melanoma-associated single-nucleotide (SNPs) in a Greek case-control population, and examine their predictive value. Methods Based on field synopsis of genetic variants melanoma (MelGene), we genotyped 284 patients controls at 34 SNPs which 19 derived from GWAS. tested each one the 33 passing quality control for with both without...
Although accumulation of lymphocytes in the white adipose tissue (WAT) obesity is linked to insulin resistance, it remains unclear whether also participate regulation energy homeostasis WAT. Here, we demonstrate enhanced dissipation Rag1-/- mice, increased catecholaminergic input subcutaneous WAT, and significant beige adipogenesis. Adoptive transfer experiments demonstrated that CD8+ T cell deficiency accounts for adipogenesis mice. Consistently, identified CD8-/- mice presented with The...
Virus-induced heterologous immunity is considered a barrier to transplantation tolerance. Yet, hepatitis C (HCV)-infected liver transplant (LT) patients occasionally achieve operational We investigated the mechanisms through which HCV infection modulates donor-specific T cell responses following LT and influence of eradication. generated lines from HCV-infected non-LT before after eradication quantified alloreactive using expressing single-HLA class-I antigens in presence/absence PD-1/CTLA-4...
CD4+CD25+Foxp3+ Tregs are known to acquire tissue-specific features and exert cytoprotective regenerative functions. The extent which this applies liver-resident is unknown. In study, we aimed explore the phenotypic functional characteristics of adult murine liver resident during homeostasis. Additionally, investigated their role in ameliorating inflammation tissue damage. Quantification Foxp3+CD4+CD25+ cells comparing different tissues showed that contained significantly fewer Tregs. A...
Cell-derived small extracellular vesicles (sEVs) participate in cell-cell communication via the transfer of molecular cargo including selectively enriched microRNAs (miRNAs). Utilizing advances sEV isolation and characterization, this study investigates impact liver injury dysfunction on circulating EV-miRNA profile.High-throughput screening 799 sEV-miRNAs isolated from plasma was performed patients across a spectrum disorders compensated decompensated chronic disease, acute-on-chronic...
Chatzinasiou, F.; Lill, C.M.; Spyrou, G.; Kypreou, K.; Stefanaki, I.; Nicolaou, V.; Evangelou, E.; Roehr, J.T.; Kodela, Katsambas, A.; Tsao, H.; Ioannidis, J.P.A.; Bertram, L.; Stratigos, A.J. Author Information
Introduction The genetic susceptibility to melanoma remains the subject of intense research worldwide, focusing on either rare, familial high-penetrance sequence variants, or more common moderate- low-risk predisposing allele. number published data associations has increased immensely over past years implying a putative risk alleles in several genes. Aim To perform comprehensive field synopsis all and create an on-line database that collects curates results this area. Methods We have...
Introduction Patients diagnosed with a primary cutaneous melanoma are in higher risk to develop subsequent melanoma, an incidence rate that varies from 1.2 8.2%. Only few studies have evaluated the incidence, factors and clinical parameters of patients multiple melanomas (MPM) Mediterranean populations. Aim To study epidemiological clinico-pathological characteristics Greek hospital-based cohort. Methods We performed retrospective medical records A. Sygros Hospital's Melanoma Unit 1998 2009....