A5072947508- Oral microbiology and periodontitis research
- Immunotherapy and Immune Responses
- Hemoglobinopathies and Related Disorders
- Protease and Inhibitor Mechanisms
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Veterinary Equine Medical Research
- T-cell and B-cell Immunology
- Heme Oxygenase-1 and Carbon Monoxide
- Oral and gingival health research
- Renal Transplantation Outcomes and Treatments
- Diabetes and associated disorders
- NF-κB Signaling Pathways
- Cancer Research and Treatments
- Erythrocyte Function and Pathophysiology
- Acute Myeloid Leukemia Research
- Periodontal Regeneration and Treatments
- Reproductive Physiology in Livestock
- Organ Transplantation Techniques and Outcomes
- Cytomegalovirus and herpesvirus research
- Blood groups and transfusion
- Advanced Glycation End Products research
- S100 Proteins and Annexins
- Complement system in diseases
- Prostate Cancer Treatment and Research
Emory University
2022-2025
Immune Tolerance Network
2024
Universidade Estadual de Campinas (UNICAMP)
2008-2022
University of Alabama at Birmingham
2020-2022
Zero to Three
2019
Universidade Federal de Minas Gerais
2010
Universidade Estadual Paulista (Unesp)
2010
Evaluate the signaling pathways associated with inflammatory mediators activated in two models of experimental periodontitis.Two were used: lipopolysaccharide (LPS) injections and ligature placement. Wistar rats used 30 microg LPS from Escherichia coli was injected twice a week into palatal aspect upper molars. Ligatures placed around lower first A control group received PBS on gingivae whereas no ligatures Samples collected 5, 15 days processed for analysis by Western blotting...
Molecular mechanisms responsible for periodontal disease (PD) and its worsening in type 1 Diabetes Mellitus (DM1) remain unknown. Cytokine profile expression levels of collagenases, Mmp14, tissue inhibitors were determined, as the numbers neutrophils macrophages combined streptozotocin-induced DM1 ligature-induced PD models. Increased IL-23 (80-fold) Mmp8 (25-fold) was found DM1. Ligature resulted an IL-1β/IL-6 profile, increased Mmp8, Mmp13, Mmp14 (but not Mmp1), transient Timp1 Reck...
Evaluate expression of MMP-13 during the course two models experimentally induced periodontal disease in rats.Expression at mRNA and protein levels was studied, respectively, by reverse transcription polymerase chain reaction (RT-PCR) Western blotting. Two experimental were used: LPS injections ligature placement. 30mug from Eschericia coli injected twice a week into palatal aspect upper molars. Ligatures placed gingival margin around lower first Controls received PBS vehicle no ligatures on...
Alveolar bone resorption results from the inflammatory response to periodontal pathogens. Systemic diseases that affect host response, such as type 1 diabetes mellitus (DM1), can potentiate severity of disease (PD) and accelerate resorption. However, biological mechanisms by which DM1 modulates PD are not fully understood. The aim this study was determine influence on alveolar evaluate role receptor activator nuclear factor-kappaB ligand (RANKL)/osteoprotegerin (OPG) in osteoclastogenesis...
Periodontal disease corresponds to a group of lesions that affect the tooth-supporting tissues present in dental follicle. Although bacterial plaque is important, immune response also contributes destruction periodontal tissues. Diabetes mellitus closely associated with development, progression and severity because it not only affects extracellular matrix organization but tissue inflammation. The objective investigation was study influence diabetes on experimental by evaluating degradation...
In periodontal disease, extensive disorganization of the extracellular matrix promotes loss adhesion between teeth and periodontium. A previous study suggested a reduction in area occupied by collagen gingiva, during first week disease induction, however, remaining fibers were more compact thicker. Therefore, it was decided to investigate which MMP-2, -9, -14 RECK, an MMP inhibitor, involved these modifications taking place early gingivitis induced ligature. The results gene expression...
Androgen deprivation results in massive apoptosis the prostate gland. Macrophages are actively engaged phagocytosing epithelial cell corpses. However, it is unknown whether microtubule-associated protein 1 light chain 3 alpha (LC3)-associated phagocytosis (LAP) involved and contribute to prevent inflammation.Flow cytometry, RT-PCR immunohistochemistry were used characterize macrophage subpopulation residing layer of rat ventral (VP) after castration. Stereology was employed determine...
Background Sickle cell disease (SCD) incurs vaso-occlusive episodes and organ damage, including nephropathy. Despite displaying characteristics of vascular dysfunction, SCD patients tend to present relatively lower systemic blood pressure (BP), via an unknown mechanism. We investigated associations between BP renin-angiotensin-system (RAS) components in determined whether inhibitor angiotensin converting enzyme (ACE; often used slow glomerulopathy) further modulates RAS a murine model SCD....
The vascular endothelial growth factor receptors (VEGFRs) can shape the neovascular phenotype of cells when translocated to nucleus, however spatial and temporal changes in intracellular distribution translocation VEGFRs nucleus organelles involved this process is unclear. This study reports effect exogenous VEGF on micro- macrovascular cells. We showed that responsible for: a rapid substantial nuclear VEGFRs; VEGFR1 VEGFR2 exhibit distinct spatial, structural characteristics both vitro vivo...
The prostate secretes immunoglobulin (Ig) A (IgA) and IgG; however, how immunoglobulins reach the secretion, where plasma cells are located, whether antigen-specific activation of adaptive response occurs still unknown. Immune cells, including CD45RA+ were scattered in stroma not organized mucosae-associated lymphoid-tissue. IgA (but IgG) immunostaining identified stromal epithelial non-immunized rats. Injected tetramethylrhodamine-IgA transcytosed epithelium along with polymeric receptor....
Abstract Prostate development and function are regulated by androgens. Epithelial cell apoptosis in response to androgen deprivation is caspase‐9‐dependent peaks at Day 3 after castration. However, isolated epithelial cells survive the absence of Znf142 showed an on‐off expression pattern intraepithelial CD68‐positive macrophages, with on‐phase Rats treated gadolinium chloride deplete macrophages a significant drop apoptosis, suggesting causal relationship between apoptosis. Intraepithelial...
Twenty horses were used in the experiment, for composed control group, (Cg) instrumented (Ig;without intestinal obstruction), treated group (Tg;submitted to obstruction and hydrocortisone treatment) non-treated (Ntg;submitted without treatment). Immunohistochemistry zymography techniques researches on MMPs 2 9 horse hoof laminae. There was an increase expression of MMP-2 animals Tg Ntg. MMP-9 increased from groups Ntg Ig, however there no rise this MMP when compared other...
A laminite é uma doença podal grave que acomete os equídeos, sendo responsável por intenso sofrimento. Neste estudo foram pesquisadas a presença de calprotectina meio da imunoistoquímica, e lipocalina associada à gelatinase neutrófilos (NGAL), zimografia, no tecido laminar do casco equinos após obstrução intestinal. Os animais divididos em quatro grupos: Grupo controle (Gc), contendo sete normais, sem procedimento cirúrgico; Instrumentado (Gi), cinco animais, quais passaram todo o cirúrgico...
Previously, we reported that the intravenous injection of bone marrow-derived cells (BMDC) infected with lentivirus expressing human RPE65 gene resulted in programming BMDC to promote visual recovery a mouse model age-related macular degeneration (AMD). The aim this study was characterize spatial and temporal recruitment these programmed retinal pigment epithelial (RPE) layer.C57BL/6J female mice received subretinal AAV1-SOD2 ribozyme knock down (KD) superoxide dismutase 2 (SOD2) induce...