Increasing epidemiologic evidence supports a link between periodontitis and rheumatoid arthritis. The actual involvement of in the pathogenesis arthritis underlying mechanisms remain, however, poorly understood. We investigated influence concomitant on clinical histopathologic characteristics T cell-mediated experimental evaluated modulation type II collagen (CII)-reactive Th cell phenotype as potential mechanism. Repeated oral inoculations periodontal pathogens Porphyromonas gingivalis...

Curcumin is a plant-derived dietary spice with various biological activities, including anticarcinogenic and anti-inflammatory effects. Its therapeutic applications have been studied in variety of conditions, rheumatoid arthritis, colon cancer depression, but no studies evaluated the effects curcumin on periodontal disease vivo.Experimental was induced rats by placing cotton ligatures around both lower first molars. given to intragastric route daily at two dosages (30 100 mg/kg) for 15 d....

Evaluate the signaling pathways associated with inflammatory mediators activated in two models of experimental periodontitis.Two were used: lipopolysaccharide (LPS) injections and ligature placement. Wistar rats used 30 microg LPS from Escherichia coli was injected twice a week into palatal aspect upper molars. Ligatures placed around lower first A control group received PBS on gingivae whereas no ligatures Samples collected 5, 15 days processed for analysis by Western blotting...

Curcumin is a plant-derived dietary spice ascribed various biological activities. therapeutic applications have been studied in variety of conditions, but not on periodontal disease. Periodontal disease chronic inflammatory condition initiated by an immune response to micro-organisms the dental biofilm. Experimental was induced rats injecting LPS gingival tissues palatal aspect upper first molars (30 µg LPS, 3 times/week for 2 weeks). administered daily via oral gavage at 30 and 100 mg/kg...

The objective of this study was to investigate the effectiveness (+)-β-pinene inhibition on Candida spp. growth, aiming at elucidation mechanism action; determine fungal cell enzyme binding activity (through molecular docking simulations) and its effects biofilm reduction.Candida strains (n=25) from referenced clinical origins, either susceptible or resistant standard antifungals, were tested for determination Minimum Inhibitory Concentration (MIC); Fungicidal (MFC); microbial death curves...

Background and Objective: Cyclosporine A treatment is important in the therapy of a number medical conditions; however, alveolar bone loss an negative side‐effect this drug. As such, we evaluated whether concomitant administration simvastatin would minimize cyclosporine A‐associated rats subjected, or not, to experimental periodontal disease. Material Methods: Groups 10 each were treated with (10 mg/kg/day), (20 concurrently (cyclosporine A/simvastatin) vehicle for 30 days. Four other groups...

Abstract Background Arachidonate‐5‐lipoxygenase (5‐LO) activity and increased leukotriene B4 (LTB4) production have been implicated in various inflammatory conditions. Increased of leukotrienes has associated with periodontal diseases; however, their relative contribution to tissue destruction is unknown. In this study, an orally active specific 5‐LO inhibitor used assess its role inflammation bone resorption a murine model lipopolysaccharide (LPS)‐induced disease. Methods Periodontal...

The present study demonstrates the antifungal potential of chemically characterized essential oil (EO) Cinnamomum zeylanicum Blume on Candida spp. biofilm and establishes its mode action, effect fungal growth kinetics, cytotoxicity to human cells. minimal inhibitory concentration (MIC) fungicidal (MFC) values varied from 62.5 1,000 μg/mL, seems be due interference with cell wall biosynthesis. kinetics assay showed that EO at MICx2 (500 μg/mL) induced a significant (p < 0.05) reduction after...

Evaluate expression of MMP-13 during the course two models experimentally induced periodontal disease in rats.Expression at mRNA and protein levels was studied, respectively, by reverse transcription polymerase chain reaction (RT-PCR) Western blotting. Two experimental were used: LPS injections ligature placement. 30mug from Eschericia coli injected twice a week into palatal aspect upper molars. Ligatures placed gingival margin around lower first Controls received PBS vehicle no ligatures on...

Abstract Aim To evaluate whether Porphyromonas gingivalis ‐induced periodontitis aggravates the antigen‐induced arthritis ( AIA ) model, and this effect is dependent on Th17/ IL ‐17 signalling pathway. Materials methods Antigen‐induced was triggered by local injection of methylated bovine serum albumin into knee joint previously immunized C57 BL /6 wild‐type WT receptor A RA )‐knockout mice. Periodontal disease in naïve or arthritic mice induced oral infection with P. . Animals were...

Aim. This study sought to investigate the in vitro expression profile of high mobility group box 1 (HMGB1) murine periodontal ligament fibroblasts (mPDL) stimulated with LPS or IL-1 β and vivo during ligature- LPS-induced periodontitis rats. Material Methods. For study, 36 rats were divided into experimental control groups, biopsies harvested at 7–30 d following disease induction. Bone loss inflammation evaluated. HMGB1 was assessed by immunohistochemistry, qPCR, Western blot. Results....

Objective The cytokine interleukin‐21 (IL‐21) can have both proinflammatory and immunosuppressive effects. purpose of this study was to investigate the potential dual role IL‐21 in experimental arthritis relation Th17 cells. Methods Antigen‐induced (AIA) chronic streptococcal cell wall (SCW) were induced receptor–deficient (IL‐21R −/− ) wild‐type mice. Knee joints, synovial tissue, serum analyzed for pathology inflammatory markers. Results During AIA SCW arthritis, IL‐21R deficiency...

Natural products have emerged as a rich source of bioactive compounds for adjunctive treatments many infectious and inflammatory conditions, including periodontitis. Among the monoterpenes with significant biological properties, there is perillyl alcohol (POH), which can be found in several essential oils has shown immunomodulatory properties recent studies, may interesting treatment non-neoplastic disorders. Objective To determine antibacterial immune modulatory activities POH. Methodology...

NOD2 is a member of the NLR family proteins that participate in activation innate immune response. RIP2 downstream kinase activated by both NOD1 and NOD2. There scarcity information regarding relevance periodontitis, chronic inflammatory condition characterized bone resorption. We used NOD2-KO RIP2-KO mice model microbial-induced periodontitis. Heat-killed Aggregatibacter actinomycetemcomitans was injected gingival tissues three times/wk for 4 wk. Bone resorption assessed μCT analysis;...

Genetic and epigenetic changes have been associated with periodontitis in various genes; however, little is known about genes involved mechanisms oxidative stress.

1. Hypoglycaemia induces glucose intolerance in normal subjects—the ‘Somogyi effect’–and may be responsible for some instances of ‘brittle diabetes’. This effect mediated through the growth hormone response to hypoglycaemia, but other possible hormonal mechanisms have not been excluded. 2. Paired 2-h oral tolerance tests carried out 2 h after both (a) i.v. saline (control day) and (b) insulin (test four subjects twenty-seven patients with pituitary adrenal under- over-activity. Plasma...

Resident, non-immune cells express various pattern-recognition receptors and produce inflammatory cytokines in response to microbial antigens, during the innate immune response.Alveolar bone resorption is hallmark of destructive periodontitis it caused by host bacteria their mediators present on biofilm.The balance between expression levels receptor activator nuclear factorkappa B ligand (RANKL) osteoprotegerin (OPG) pivotal for osteoclast differentiation activity has been implicated...

To evaluate the influence of use avocado/soybean unsaponifiables (ASU) on osseointegration implants in animals with experimental arthritis.One hundred twenty rats were randomly divided into four groups: CTR, healthy and saline solution administration; ASU, ASU ART, arthritic ART/ASU, administration. The solutions administered daily by gavage, beginning 7 days before surgical procedures until completion period (15, 30, 60 after placement tibia). was evaluated histometric analysis...

Abstract Background Antimicrobial peptides are components of innate immune response that have a key role on susceptibility and resistance the oral cavity to diseases. This study aimed investigate influence smoking cathelicidin LL‐37 human neutrophil 1 through 3 (HNP 1‒3) levels in gingival crevicular fluid (GCF) patients with periodontitis. The relationship between these periodontal status selected inflammatory mediators smokers non‐smokers was also evaluated. Methods Forty periodontitis, 20...

This study evaluated the in vitro antimicrobial and immunomodulatory action of crude extracts from Anacardium occidentale L. (cashew tree) leaves bark, to determine their toxicity peripheral-blood mononuclear cells (PBMCs) zebrafish embryos larvae. Chemical analysis was performed by proton nuclear magnetic resonance (1H-NMR). The antibacterial activity against selected bacteria strains determining minimum inhibitory concentration (MIC) bactericidal (MBC). Cytotoxicity assessed using...