A5089365665- Oral microbiology and periodontitis research
- Immune Response and Inflammation
- Dental Implant Techniques and Outcomes
- Inflammasome and immune disorders
- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Dental materials and restorations
- Galectins and Cancer Biology
- Pancreatic and Hepatic Oncology Research
- Streptococcal Infections and Treatments
- Pancreatitis Pathology and Treatment
- Neuroendocrine Tumor Research Advances
- Macrophage Migration Inhibitory Factor
- Neuroblastoma Research and Treatments
- Dental Trauma and Treatments
- Sphingolipid Metabolism and Signaling
- Curcumin's Biomedical Applications
- Bone and Dental Protein Studies
- Cancer Research and Treatments
- Salivary Gland Disorders and Functions
- Bone health and treatments
- Bacterial Infections and Vaccines
- Appendicitis Diagnosis and Management
- Lung Cancer Research Studies
- Pharmacological Effects of Natural Compounds
University of Florida
2016-2024
Florida College
2021-2022
Universidade Estadual Paulista (Unesp)
2011-2019
Centro Universitário de Araraquara
2016-2019
Meharry Medical College
2019
Universidade de São Paulo
2019
Vanderbilt University Medical Center
2018
The University of Texas MD Anderson Cancer Center
2018
University of Florida Health Science Center
2016
Brigham and Women's Hospital
2005
Abstract There is evidence indicating that curcumin has multiple biological activities, including anti-inflammatory properties. In vitro and in vivo studies demonstrate may attenuate inflammation the connective tissue destruction associated with periodontal disease. Most of these use systemic administration, considering site-specific nature disease also poor pharmacodynamic properties curcumin, we conducted this proof principle study to assess effect local administration a nanoparticle...
Abstract Background Piezosurgery is an osteotomy system used in medical and dental surgery. Manystudies have proven clinical advantages of piezosurgery terms quality ofcut, maneuverability, ease use, safety. However, few investigations havetested its superiority over the traditional systems dynamicsof bone healing. Therefore, aim this study was to evaluate dynamics ofbone healing after osteotomies with compare them thoseassociated drilling. Methods One hundred ten rats were divided into two...
The treatment of chronic inflammation with systemically administered anti-inflammatory treatments is associated moderate-to-severe side effects, and the efficacy locally drugs short-lived. Here we show that can be suppressed by a fusion protein immunosuppressive enzyme indoleamine 2,3-dioxygenase 1 (IDO) galectin-3 (Gal3). Gal3 anchors IDO to tissue, limiting diffusion IDO-Gal3 away from injection site. In rodent models endotoxin-induced inflammation, psoriasis, periodontal disease...
Abstract This study investigates the role of NLRP3 inflammasome and its main effector Caspase-1 in inflammation alveolar bone resorption associated with periodontitis. Heat-killed Aggregatibacter actinomycetemcomitans (Aa) was injected 3x/week (4 weeks) into gingival tissues wild-type (WT), Nlrp3 -KO Caspase1 mice. Bone measured by µCT osteoclast number determined tartrate-resistant acid phosphatase (TRAP) staining. Inflammation assessed histologically (H/E staining immunofluorescence CD45...
Cancer cachexia represents a debilitating syndrome that diminishes quality of life and augments the toxicities conventional treatments. is particularly in patients with pancreatic cancer (PC). Mechanisms responsible for are under investigation largely derived from observations syngeneic murine models which limited PC. We evaluate effect human PC cells on both muscle wasting systemic inflammatory milieu potentially contributing to PC-associated cachexia. Specifically, xenografts were...
Sphingolipids (SLs) are essential structural components of mammalian cell membranes. Our group recently determined that the oral anaerobe Porphyromonas gingivalis delivers its SLs to host cells and ability P. synthesize limits elicited inflammatory response during cellular infection. As robustly produces outer membrane vesicles (OMVs), we hypothesized OMVs serve as a delivery vehicle for SLs, SL status may impact cargo loading OMVs, SL-containing limit inflammation similar observed by direct...
Porphyromonas gingivalis, like other bacteria belonging to the phylum Bacteroidetes, synthesizes sphingolipids (SLs). However, their exact roles in microbial physiology and potential role mediating interactions with eukaryotic host are unclear. Our working hypothesis for this study was that synthesis of SLs (host-like lipids) affords a mechanism allows P. gingivalis persist homeostasis its host. In previous study, we deleted gene (PG1780 strain W83) predicted encode serine palmitoyl...
This study investigated structural and functional features of apoptotic alveolar bone osteoclasts in estrogen-treated rats. For this purpose, 15 female rats 22 days old were divided into three groups: Estrogen (EG), Sham (SG) Control (CG). The EG received daily intramuscular injection estrogen for 7 days. SG only the oil vehicle. Maxillary fragments containing removed processed light transmission electron microscopy. Area (OcA) number nuclei (OcN) resorption surface per TRAP-positive (BS/OC)...
NOD2 is a member of the NLR family proteins that participate in activation innate immune response. RIP2 downstream kinase activated by both NOD1 and NOD2. There scarcity information regarding relevance periodontitis, chronic inflammatory condition characterized bone resorption. We used NOD2-KO RIP2-KO mice model microbial-induced periodontitis. Heat-killed Aggregatibacter actinomycetemcomitans was injected gingival tissues three times/wk for 4 wk. Bone resorption assessed μCT analysis;...
Sphingoid base phosphates, such as s phingosine- 1 - p hosphate (S1P) and d i h ydro (dhS1P), act ligands for S1P receptors, this interaction is known to play a central role in mediating angiogenesis, vascular stability permeability, immune cell migration sites of inflammation. Studies suggest that shift ratio higher levels dhS1P relation alters downstream signaling cascades due differential binding activation the various receptor isoforms.
Peri-implantitis is a major cause of dental implant failure. This disease an inflammation the tissues surrounding implant, and, while multi-factorial, bacteria main culprit in initiating inflammatory reaction. Dental implants with silicon carbonitride (SiCN) coatings have several potential advantages over traditional titanium implants, but their antibacterial efficiency has not yet been evaluated. The purpose this study was to determine anti-bacterial SiCN by modifying surface SiCN-coated...
The demand for dental implants has increased, establishing them as the standard of care replacing missing teeth. Several factors contribute to success or failure an implant post-placement. Modifications surfaces can enhance biological interactions between bone cells and implant, promoting better outcomes. Surface coatings have been developed electrochemically alter surfaces, aiming reduce healing time, growth, prevent bacterial adhesion. Quaternized silicon carbon nitride (QSiCN) is a novel...
The objective of this study was to evaluate the influence titanium nanotube diameter and effect silicon carbide (SiC) coating on proliferation mineralization pre-osteoblasts nanostructured surfaces. Anodized sheets with diameters 50 100 nm were used. following four groups tested in study: (1) non-coated nanotubes; (2) SiC-coated (3) nanotubes (4) nanotubes. biocompatibility cytotoxicity evaluated using a CellTiter-BlueCell Viability assay after 1, 2, 3 days. After days, cells attached...
Periodontal diseases are chronic inflammatory of the periodontium that result in progressive destruction soft and hard tissues supporting teeth, it is most common cause tooth loss among adults. In US alone, over 100 million individuals estimated to have periodontal disease. Subgingival bacteria initiate sustain inflammation, and, although several been associated with periodontitis, Porphyromonas gingivalis has emerged as key etiological organism significantly contributing Currently,...
Mechanical instrumentation of the root surface causes formation a smear layer, which is physical barrier that can affect periodontal regeneration. Although different procedures have been proposed to remove there no information concerning how long layer persists on surfaces after in vivo. This study assessed presence over 28-day period subgingival with hand instruments. Fifty human teeth were referred for extraction because advanced disease scaled and planed (SRP) by single experienced...
This study aimed to comprehensively assess the influence of nanotube diameter and presence a silicon carbide (SiC) coating on microbial proliferation nanostructured titanium surfaces. An experiment used 72 anodized sheets with varying diameters 50 100 nm. These were divided into four groups: non-coated nm nanotubes, SiC-coated totaling 36 samples per group.
Periodontal regeneration is still a challenge in terms of predictability and magnitude effect. In this study we assess the biological effects combining chemical root conditioning mediators on three relevant cell types for periodontal regeneration. Bovine dentin slices were conditioned with 25% citric acid followed by topical application basic fibroblast growth factor (bFGF, 10 50 ng). We used ELISA to dynamics bFGF release from surface RT-qPCR expression Runx2, Col1a1, Bglap fibronectin...
The purpose of this in vitro study was to quantify the alterations on human root dentin permeability after exposure different acid fruit juices and evaluate effect toothbrushing with electric or sonic toothbrush exposure. 50 extracted third molars exposed a high speed bur. Crowns were sectioned above cementoenamel junction fragments used prepare specimens. Specimens randomly assigned 5 groups according juice (kiwifruit, starfruit, green apple, pineapple acerolla). Each specimen connected...
Orthodontic treatment consists of numerous appliance activations that rely on stimulation osteoclasts at alveolar bone sites. However, the action osteoclast-like cells dentin ("odontoclasts") is a pathological side effect orthodontic treatment. The aim this article twofold: (a) To report preliminary results from ongoing cell culture experiments to identify unique markers resorption, and (b) discuss our work using nanoparticle tracking analysis (NTA) exosomes for developing biological...
Summary paragraph Chronic inflammation underlies the onset, progression and associated pain of numerous diseases.( 1 ) Current anti-inflammatory treatments administered systemically are with moderate-to-severe side effects, while locally drugs have short-lived efficacy, neither approach successfully modifies underlying causality disease.( 2 We report a new way to modulate by fusing enzyme indoleamine 2,3-dioxygenase (IDO) galectin-3 (Gal3). A general regulator inflammation( 3 ), IDO is...