This study analysed the status of DNA methylation in promoter region IL8 gene oral mucosa cells from healthy, smoker and non-smoker subjects with chronic periodontitis compared these findings among groups mRNA levels.Genomic epithelial 41 healthy subjects, 30 smokers 40 non-smokers were purified modified by sodium bisulphite. Genomic blood leucocytes gingival biopsies 13 each group also Modified was submitted methylation-specific polymerase chain reaction (PCR) (MSP), electrophoresed on 10%...

ABSTRACT Although, intracellular signaling pathways are proposed to predict the quality of cell‐surface relationship, this study addressed pre‐osteoblast behavior in response nano hydroxyapatite (HA)‐blasted titanium (Ti) surface by exploring critical and morphological change. Physicochemical properties were evaluated atomic force microscopy (AFM) wettability considering water contact angle three differently texturized Ti surfaces: Machined (Mac), Dual acid‐etching (DAE),...

Background: Cigarette smoking has been shown to negatively influence healing following periodontal therapeutic procedures. Therefore, the aim of this study was evaluate impact on clinical outcome root coverage subepithelial connective tissue graft (CTG) surgery. Methods: Eighteen defects were treated in 15 patients (seven smokers and eight non‐smokers) who presented canine premolar Miller Class I II recessions. CTG performed measurements obtained at baseline, 30, 60, 90, 120 days after...

Background: Studies evaluating the methylation status of cytokine genes may have relevance for inflammatory diseases in which expression some cytokines is altered, such as periodontitis. This study observes DNA interleukin‐8 (IL8) gene promoter cells oral epithelium subjects affected by generalized aggressive periodontitis (AgP) and compares it to those control subjects. Methods: Genomic from epithelial 37 AgP patients controls were purified modified sodium bisulphite. Modified was submitted...

Background: The proinflammatory chemokine interleukin (IL)‐8 is important in the regulation of inflammatory response. Analyses single nucleotide polymorphism (SNP) reference sequence (rs) 4073 showed that A allele upregulated IL‐8 levels after stimulation with lipopolysaccharides. We investigated association SNP rs4073 chronic periodontitis. Methods: Genotyping was performed by a standard polymerase chain reaction–restriction fragment length assay 289 genomic DNA samples healthy control...

De Oliveira NFP, Andia DC, Planello AC, Pasetto S, Marques MR, Nociti FH, Line SRP, Souza, AP. TLR2 and TLR4 gene promoter methylation status during chronic periodontitis. J Clin Periodontol 2011; 38: 975–983. doi: 10.1111/j.1600‐051X.2011.01765.x Abstract Aim: The objective of this study was to analyse the DNA in region toll‐like receptor ( TLR ) 2 genes gingival tissue samples from healthy subjects, smokers non‐smokers affected by Material Methods: Genomic total RNA were purified using...

Background: The aim of this clinical study was to evaluate the association locally delivered doxycycline 10% with scaling and root planing in periodontal treatment smokers during a 2‐year period. Methods: Forty‐eight chronic periodontitis patients, presenting minimum four pockets (≥5 mm) that bled on probing anterior teeth were included. Patients randomly assigned receive one following treatments: (SRP) followed by local application (SRP‐D). Assigned treatments performed at baseline 12...

Background and Objective: Cyclosporine A treatment is important in the therapy of a number medical conditions; however, alveolar bone loss an negative side‐effect this drug. As such, we evaluated whether concomitant administration simvastatin would minimize cyclosporine A‐associated rats subjected, or not, to experimental periodontal disease. Material Methods: Groups 10 each were treated with (10 mg/kg/day), (20 concurrently (cyclosporine A/simvastatin) vehicle for 30 days. Four other groups...

Periodontal ligament cells (PDLCs) have well documented osteogenic potential; however, this commitment can be highly heterogenous, limiting their applications in tissue regeneration. In study, we use PDLC populations characterized by high and low potential (h-PDLCs l-PDLCs, respectively) to identify possible sources of such heterogeneity investigate whether the differentiation enhanced epigenetic modulation. h-PDLCs, basal expression levels pluripotency markers (NANOG, OCT4), DNA...

Abstract Whereas endothelial responses to shear stress are well‐characterized, the cell physiological effects of in smooth muscle cells (SMCs) remain largely obscure. As SMCs directly challenged by after denuding injury following procedures such as angioplasty or endarterectomy, characterization these represents an important scientific question. Hence we decided contrast cytoskeletal reorganization, epigenetic reprogramming, signaling transduction, and changes miRNA (miRs) profiles primary...

Aim: This study aimed to evaluate the ability of human periodontal ligament stem cells (PDLSCs) with high (HP-PDLSCs) and low (LP-PDLSCs) osteogenic potential, in addition mixed cells, repair bone tissue. Methods: Cell phenotype, proliferation differentiation were evaluated. Undifferentiated PDLSCs injected into rat calvarial defects new was evaluated by μCT, histology real-time PCR. Results: exhibited a typical mesenchymal cell phenotype HP-PDLSCs showed lower proliferative higher potential...

Although subepithelial connective tissue graft (CTG) has been reported to be a predictable procedure for root coverage, the impact of smoking on long-term outcome periodontal plastic surgery is unclear. Hence, aim this study was evaluate effect smoking, basis, stability gingival following CTG treatment recession.Twenty-two defects were treated by in canine and premolar Miller Class I II recessions (11 smokers 11 non-smokers). The clinical measurements obtained at baseline 1, 2, 3, 4, 6, 12,...

Human bone marrow-derived mesenchymal stem cells (hBMSCs) are important for tissue regeneration but their epigenetic regulation is not well understood. Here we investigate the ability of a non-nucleoside DNA methylation inhibitor, RG108 to induce changes at both global and gene-specific levels in order enhance cell markers, hBMSCs. hBMSCs were treated with complete culture medium, 50 μM DMSO three days subjected viability apoptosis assays, methylation/hydroxymethylation, transcript levels'...

The aim of the present study was to evaluate effect hydroxyethyl-methacrylate (HEMA) concentration and solvent content dental adhesives on cell viability cytokine (IL-1b, IL-6, IL-10, TNF-α) release by human pulp cells (HDPCs). HDPCs were obtained from fresh extracted third molars. Experimental prepared containing different concentrations HEMA (0%, 10%, 20%) with without (ethanol 10%). Cylindrical specimens immersed culture medium during 24 h obtain extracts. incubated extracts (culture +...

Periodontal ligament stem cells (PDLCs) can be used as a valuable source in cell therapies to regenerate bone tissue. However, the potential therapeutic outcomes are unpredictable due PDLCs’ heterogeneity regarding capacity for osteoblast differentiation and mineral nodules production. Here, we identify epigenetic (DNA (hydroxy)methylation), chromatin (ATAC-seq) transcriptional (RNA-seq) differences between PDLCs presenting with low (l) high (h) osteogenic potential. The primary populations...

Background: The aim of this clinical study was to evaluate the association locally delivered doxycycline (10%) with scaling and root planing in periodontal treatment smokers. Methods: Forty‐three patients chronic periodontitis a minimum four pockets (≥5 mm) on anterior teeth that bled probing were selected. Patients randomly assigned (SRP) or followed by local application (SRP‐D). Plaque, bleeding probing, gingival recession, relative attachment level RAL), depth (PD) recorded at baseline,...