Background: Human postnatal stem cells have been identified in periodontal ligaments (PDLs). In this study, the vitro biologic properties of CD105 + enriched cell subsets from PDLs harvested deciduous (DePDL) and permanent (PePDL) teeth are comparatively assessed. Methods: PDL tissue was obtained 12 (six primary six permanent) which CD34 − CD45 were isolated by magnetic sorting. To identify quantitatively compare markers, DePDL PePDL assessed for CD166 surface antigen expression flow...

Silvério KG, Davidson KC, James RG, Adams AM, Foster BL, Nociti FH Jr, Somerman MJ, Moon RT. Wnt/β‐catenin pathway regulates bone morphogenetic protein (BMP2)‐mediated differentiation of dental follicle cells. J Periodont Res 2012; 47: 309–319. © 2011 John Wiley & Sons A/S Background and Objective: Bone 2 (BMP2)‐induced osteogenic has been shown to occur through the canonical Wnt/βcatenin pathway, whereas factors promoting Wnt signaling in cementoblasts inhibit cell promote proliferation...

Abstract Aim This study aimed to evaluate, histomorphometrically, the use of periodontal ligament cells ( PDL cells) in treatment class III furcation defects. Material and Methods were obtained from mandibular tooth extracted each dog (7), cultured vitro phenotypically characterized. Bilateral defects created at 3rd 4th premolars randomly assigned to: Control Group: coronally positioned flap, GTR , Sponge carrier + Cell . Results After 3 months healing, data analysis demonstrated that Group...

Background/Objectives: Dental caries remains a prevalent chronic disease globally, driven by complex interactions between the host, diet, and microbial communities. This study employs metatranscriptomic RNA-Seq analysis to explore functional dynamics of dentin microbiome in both healthy carious teeth. By examining transcriptional activity bacterial communities, we aimed identify key species molecular functions associated with progression. Methods: Samples from six patients (three three...

Background: Gingival recession (GR) might be associated with patient discomfort due to cervical dentin hypersensitivity (CDH) and esthetic dissatisfaction. The aim is evaluate the effect of root coverage procedure a xenogenous collagen matrix (CM) and/or enamel derivative (EMD) in combination coronally advanced flap (CAF) on CDH, esthetics, oral health–related quality life (OHRQoL) patients GR. Methods: Sixty‐eight participants single Miller Class I/II GRs were treated CAF (n = 17), + CM EMD...

Abstract Early acquisition of a pathogenic microbiota and the presence dysbiosis in childhood is associated with susceptibility to familial aggregation periodontitis. This longitudinal interventional case–control study aimed evaluate impact parental periodontal disease on oral pathogens their offspring. Subgingival plaque clinical metrics were collected from 18 parents history generalized aggressive periodontitis children (6–12 years age), periodontally healthy at baseline following...

Periodontal ligament mesenchymal stem cells (PDLMSCs) are an important alternative source of adult and may be applied for periodontal tissue regeneration, neuroregenerative medicine, heart valve engineering. However, little is known about the impact bacterial toxins on biological properties PDLSMSCs, including self-renewal, differentiation, synthesis extracellular matrix. Objective : This study investigated whether proliferation, expression pro-inflammatory cytokines, osteogenic...

Abstract Background Mesenchymal stem cells differentiate into distinct mesenchymal cell lineages and regulate the immune response. The aim of this study was to determine whether periodontal ligament‐derived (PDLSCs) have ability modulate neutrophil responses via paracrine mechanisms. Methods CD105‐enriched PDLSCs were seeded for 24 h challenged with Porphyromonas gingivalis total protein extract ( Pg PE) (0 or 2 ug/mL) 3 h. Cells then washed further cultured 18 supernatants collected stored....

Periodontal ligament cells (PDLCs) have well documented osteogenic potential; however, this commitment can be highly heterogenous, limiting their applications in tissue regeneration. In study, we use PDLC populations characterized by high and low potential (h-PDLCs l-PDLCs, respectively) to identify possible sources of such heterogeneity investigate whether the differentiation enhanced epigenetic modulation. h-PDLCs, basal expression levels pluripotency markers (NANOG, OCT4), DNA...

Periodontal ligament cells (PDLC) play a major role in periodontal tissues homeostasis and destruction. Most age-associated diseases seem to be closely related an underlying chronic inflammatory state. Thus, the present study aimed at evaluating PDLC effect of aging on basal levels bone-related genes. Primary cultures were obtained from subjects aged 15-20 years (control- n=5), more than 60 (test- n=5). Proliferation, cell viability total secreted protein assays performed, mRNA...

Aim: This study aimed to evaluate the ability of human periodontal ligament stem cells (PDLSCs) with high (HP-PDLSCs) and low (LP-PDLSCs) osteogenic potential, in addition mixed cells, repair bone tissue. Methods: Cell phenotype, proliferation differentiation were evaluated. Undifferentiated PDLSCs injected into rat calvarial defects new was evaluated by μCT, histology real-time PCR. Results: exhibited a typical mesenchymal cell phenotype HP-PDLSCs showed lower proliferative higher potential...

Along with poor implant-bone integration, peri-implant diseases are the major causes of implant failure. Although such primarily triggered by biofilm accumulation, a complex inflammatory process in response to corrosive-related metallic ions/debris has also been recognized as risk factor. In this regard, boosting titanium (Ti) surface silane-based positive charges, cationic coatings have gained increasing attention due their ability kill pathogens and may be favorable for corrosion...

The majority of patients eligible for periodontal regenerative therapies are aged subjects. Since ligament cells (PDLC) essential regeneration, the aim present study was to determine effect cellular aging on PDLC, including genes associated with extracellular matrix metabolism and growth-associated factors. PDLC cultures were obtained from subjects 15 20 years more than 60 years. Proliferation, cell viability, mineralization assays, mRNA levels assessed type I III collagen, platelet-derived...

Suaid FF, Ribeiro FV, Rodrigues TL, Silvério KG, Carvalho MD, Nociti Jr. FH, Casati MZ, Sallum EA: Autologous periodontal ligament cells in the treatment of class II furcation defects: a study dogs. J Clin Periodontol 2011; 38: 491–498. doi: 10.1111/j.1600-051X.2010.01715.x Abstract Aim: The goal this was to histologically investigate use (PDL cells) tissue engineering regenerate defects. Material and Methods: PDL were obtained from mandibular tooth extracted each dog (seven), cultured vitro...

Abstract Objective To clinically evaluate proximal furcations treated with hydroxyapatite/β‐tricalcium phosphate ( HA /β‐ TCP ) isolated or combined enamel matrix derivative EMD ). Material and Methods Thirty patients, presenting at least one class II furcation defect, probing pocket depth PPD ≥5 mm bleeding on probing, were included. The defects assigned to the group n = 15); open‐flap debridement OFD + filling, or, ‐ filling. Plaque PI gingival index GI ), , relative margin position RGMP...

Background: It is known that periodontal ligament (PDL) harbors a heterogeneous progenitor cell population at different stages of lineage commitment. However, characterization PDL stem cells committed to osteoblast/cementoblast (O/C) differentiation remains be elucidated. The present study carried out isolate single cell–derived, cluster (CD)105–positive clones and characterize the high potential differentiate toward O/C phenotype in vitro. Methods: Isolation cell–derived colonies (clones)...

Human bone marrow-derived mesenchymal stem cells (hBMSCs) are important for tissue regeneration but their epigenetic regulation is not well understood. Here we investigate the ability of a non-nucleoside DNA methylation inhibitor, RG108 to induce changes at both global and gene-specific levels in order enhance cell markers, hBMSCs. hBMSCs were treated with complete culture medium, 50 μM DMSO three days subjected viability apoptosis assays, methylation/hydroxymethylation, transcript levels'...

Abstract Aim Generalized aggressive periodontitis ( GAP ) is a severe and multifactorial disease in which familial aggregation specific microbiological profile have been suggested. Thus, this case–control study evaluated the clinical subgingival microbial of subjects their families compared to healthy families. Methods Fifteen with parents presenting periodontal health 15 history were selected. Each family should at least one child between 6 12 years old. Plaque index PI ), gingival GI...