The virulence of the dental caries pathogen Streptococcus mutans relies in part on sucrose-dependent synthesis and interaction with glucan, a major component extracellular matrix tooth biofilms. However, mechanisms by which secreted and/or cell-associated glucan-binding proteins (Gbps) produced S. participate biofilm growth remain to be elucidated. In this study, we further investigate GbpB, an essential immunodominant protein similarity murein hydrolases. A conditional knockdown mutant that...

The two-component system VicRK and the orphan regulator CovR of Streptococcus mutans co-regulate a group virulence genes associated with synthesis interaction extracellular polysaccharides biofilm matrix. Knockout mutants vicK covR display abnormal cell division morphology phenotypes, although gene function defects involved are as yet unknown. Using transcriptomic comparisons between parent strain UA159 (UAvic) or (UAcov) deletion together electrophoretic motility shift assays (EMSA), we...

Periodontitis is a disease that leads to bone destruction and represents the main cause of tooth loss in adults. The development aggressive periodontitis has been associated with increased inflammatory response induced by presence subgingival biofilm containing Aggregatibacter actinomycetemcomitans. flavonoid quercetin (1) widespread vegetables fruits exhibits many biological properties for possible medical clinical applications such as its anti-inflamatory antioxidant effects. Thus, present...

The VicRK 2-component system of Streptococcus mutans regulates genes associated with cell wall biogenesis and biofilm formation. A putative RNase III–encoding gene ( rnc) is located downstream from the vicRKX operon. goals this study were to investigate potential role VicR in regulation adjacent evaluate transcription levels vicR during planktonic growth. Quantitative real-time polymerase chain reaction (qRT-PCR) was used whether cotranscribed. Binding purified recombinant protein promoter...

Transmission of Streptococcus mutans, a major dental caries pathogen, occurs mainly during the first 2.5 years age. Children appear to acquire S. mutans mostly from their mothers, but few studies have investigated non-familial sources transmission. This study prospectively analysed initial oral colonization in 119 children nursery schools 1.5-year period and tracked transmission child child, day-care caregiver mother child. were examined at baseline, when they 5-13 months age, 6-month...

Abstract Background Mesenchymal stem cells differentiate into distinct mesenchymal cell lineages and regulate the immune response. The aim of this study was to determine whether periodontal ligament‐derived (PDLSCs) have ability modulate neutrophil responses via paracrine mechanisms. Methods CD105‐enriched PDLSCs were seeded for 24 h challenged with Porphyromonas gingivalis total protein extract ( Pg PE) (0 or 2 ug/mL) 3 h. Cells then washed further cultured 18 supernatants collected stored....

Background: Streptococcus mutans , a major dental caries pathogen, expresses several virulence genes that mediate its growth, accumulation on tooth surfaces, and acid‐mediated demineralization. GtfB GtfC catalyze the extracellular synthesis of water‐insoluble glucan matrix from sucrose, are essential for bacteria in biofilm. GbpB, an protein S. might also cell‐surface interaction with glucan. Aim/methods: In this study, we determined transcription levels gtfB gtfC gbpB putative...

Background: Microbial agents in root canal systems can induce periodontal inflammation. The aims of this study are to detect anaerobic microorganisms endodontic‐periodontal lesions, determine the genetic diversity among them, and assess simultaneous colonization pulp microenvironments by a single clone. Methods: Twenty‐seven teeth patients with lesions were selected. Samples spread on an agar‐blood medium, detection each species was performed using polymerase chain reaction, determination...

Streptococcus sanguinis is a commensal pioneer colonizer of teeth and an opportunistic pathogen infectious endocarditis. The establishment S. in host sites likely requires dynamic fitting the cell wall response to local stimuli. In this study, we investigated two-component system (TCS) VicRK (VicRKSs), which regulates genes biogenesis, biofilm formation, virulence pathogens. A vicK knockout mutant obtained from strain SK36 (SKvic) showed slight reductions aerobic growth resistance oxidative...

ABSTRACT Streptococcus mutans , a major pathogen of dental caries, may promote systemic infections after accessing the bloodstream from oral niches. In this study, we investigate pathways complement immunity against S. and show that orphan regulator CovR (CovR Sm ) modulates susceptibility to opsonization survival in blood. blood isolates showed reduced C3b deposition compared isolates. Reduced expression covR strains was associated with increased transcription -repressed genes required for...

Meulman T, Peruzzo DC, Stipp RN, Gonçalves PF, Sallum EA, Casati MZ, Goncalves RB, Nociti FH Jr. Impact of Porphyromonas gingivalis inoculation on ligature-induced alveolar bone loss. A pilot study in rats. J Periodont Res 2011; 46: 629–636.©2011 John Wiley & Sons A/S Background and Objective: Periodontitis is a polymicrobial infection characterized by the loss connective tissue attachment, periodontal ligament bone. The aim this was to evaluate impact (ABL) model Material Methods: Forty...

Summary Streptococcus mutans , a dental caries pathogen, can promote systemic infections upon reaching the bloodstream. The two‐component system (TCS) VicRK Sm of S. regulates synthesis and interaction with sucrose‐derived exopolysaccharides (EPS), processes associated oral virulence. In this study, we investigated mechanisms by which affects susceptibility to blood‐mediated immunity. Compared parent strain UA159, vicK isogenic mutant (UAvic) showed reduced deposition C3b complement, low...

Streptococcus sanguinis is a pioneer species of teeth and common opportunistic pathogen infective endocarditis. In this study, we identified two-component system, S. SptRS (SptRS Ss ), affecting survival in saliva biofilm formation. Isogenic mutants sptRSs (SKsptR) sptSSs (SKsptS) showed reduced cell counts ex vivo assays viability compared to those parent strain SK36 complemented mutants. Reduced the were associated with growth rates nutrient-poor medium (RPMI) increased susceptibility...

Emerging antibiotic resistance in the oropharyngeal microbiota, of which Streptococcus salivarius is a prominent species, represents challenge for treating paediatric populations. In this study, we investigated role Streptococcussalivarius as reservoir genes (ARG) oral microbiota by analysing 95 isolates from 22 healthy infants (2-16 months age). MICs penicillin G, amoxicillin, erythromycin, tetracycline, doxycycline and streptomycin were determined. ARG profiles assessed subset 21 strains...

Streptococcus mutans are members of the oral microbiota that implicated in dental caries and infective endocarditis. To adapt to environmental stresses encountered during host colonization, these bacteria employ two-component regulatory systems, which modulate global changes gene expression. These include systems VicRK CovR. In this study, we investigate influence CovR S. interactions with mononuclear polymorphonuclear (PMN) phagocytes.Patterns uptake by murine macrophages were determined...

The use of antimicrobial monomers, linked to the polymer chain resin composites, is an interesting approach circumvent effects bacteria on dental and material surfaces. In addition, it can likely reduce incidence recurrent caries lesions. aim this study was evaluate a novel Triclosan Methacrylate (TM) monomer, which developed incorporated into experimental composite, Streptococcus mutans (S. mutans) biofilms, focusing analyses vicR, gtfD, gtfC, covR, gbpB gene expression, cell viability...

Mechanisms underlying systemic infections by oral species of Mitis (Streptococcus mitis, Streptococcus oralis) and Sanguinis gordonii, sanguinis) commensal streptococci are poorly understood. This study investigates profiles susceptibility to complement-mediated host immunity in representative strains these four species, which were isolated from sites or the bloodstream.Deposition complement opsonins (C3b/iC3b), surface binding C-reactive protein (CRP) IgG antibodies quantified flow...

Resinous infiltrants are indicated in the treatment of incipient carious lesions, and further development these materials may contribute to greater control lesions. The aim this study was analyze physical antibacterial properties experimental containing iodonium salt chitosan. Nine were formulated by varying concentration diphenyliodonium (DPI) at 0, 0.5 1 mol%; chitosan 0.12 0.25 g%. contained monomeric base triethylene glycol dimethacrylate bisphenol-A ethoxylate a 75 25% proportion...

This study evaluated antimicrobial activity of atorvastatin, pravastatin, rosuvastatin, and simvastatin against oral bacteria, the interaction with standard antimicrobials (amoxicillin metronidazole).

<i>Streptococcus mutans</i> is an oral bacterium considered to play a major role in the development of dental caries. This study aimed investigate prevalence <i>S. active and arrested dentine carious lesions children with early childhood caries examine expression profile selected genes associated survival virulence, within same lesions. Dentine samples were collected from 29 16 that diagnosed preschool aged 2-5 years. Total RNA was extracted samples, reverse transcription...