A5084126375- Circadian rhythm and melatonin
- Sleep and Wakefulness Research
- Spaceflight effects on biology
- Neuroendocrine regulation and behavior
- Sleep and related disorders
- Oral microbiology and periodontitis research
- Stress Responses and Cortisol
- Birth, Development, and Health
- Dietary Effects on Health
- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Neurotransmitter Receptor Influence on Behavior
- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Receptor Mechanisms and Signaling
- Diet and metabolism studies
- Neuroscience of respiration and sleep
- Photoreceptor and optogenetics research
- RNA and protein synthesis mechanisms
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Neuroscience and Neuropharmacology Research
- Bacterial Genetics and Biotechnology
- Plant nutrient uptake and metabolism
- Streptococcal Infections and Treatments
Xperi (United States)
2024
University of Kentucky
2015-2024
Albert B. Chandler Hospital
1993-2004
Lung Institute
2004
The Ohio State University
2004
Philipps University of Marburg
2004
Novant Health Forsyth Medical Center
1990-1999
University at Buffalo, State University of New York
1996-1997
University of Florida
1994
Boston University
1994
The invasion of gingival epithelial cells by certain pathogenic periodontal bacteria may account for their presence within diseased tissue. To dissect the initial steps a potential pathway pathogen Porphyromonas gingivalis, laboratory and clinical bacterial isolates were tested interactions with human oral cell line (KB). Several P. gingivalis strains immobilized on filters could bind cells. Quantitative adherence assays supported these results. 33277 was measured assay confirmed...
Abstract This study investigated whether photoperiod‐induced changes in circulating prolactin levels, which have been observed the Djungarian hamster (Yellon and Goldman, '83; Duncan '83a), might be involved seasonal pelage color this species. Injection of ovine (100 μg/day) inhibited short winter molt. finding indicated that suppression endogenous levels normally occuring photoperiod‐housed hamsters (Duncan '83a) may induce Suppression secretion with bromoergocryptine (200 strongly spring...
A mutant of Rhizobium meliloti selected as unable to grow on L-arabinose also failed acetate or pyruvate. It grew, but slower than the parental strain, many other carbon sources. Assay showed it lack alpha-ketoglutarate dehydrogenase (kgd) activity, and revertants normal growth phenotype contained activity again. Other enzymes tricarboxylic acid cycle glyoxylate were present in both parent strains. Enzymes pyruvate metabolism assayed. L-Arabinose degradation R. was found differ from known...
These studies investigated the characteristics and regional distribution of 2-[125I]iodomelatonin binding in Djungarian hamster brain. The results showed that labels two types sites, which resemble ML-1 ML-2 melatonin subtypes previously described other tissues. site identified whole brain membranes has a nanomolar affinity (Kd = 1.48 ± 0.26 nM) biochemical pharmacological identical to those Syrian hypothalamus picomolar 43.4 5.1 pM) resembles chicken retina. localization labeling...
Studies in a variety of seasonally breeding mammals have shown that melatonin mediates photoperiodic effects on reproduction. Relatively little is known, however, about the site(s) or mechanisms action this hormone for inducing reproductive effects. Although binding sites [3H] been reported previously bovine, rat, and hamster brain, pharmacological selectivity these was never demonstrated. In present study, we characterized new radioligand, 2-[125I]iodomelatonin, brains from species, Syrian...
Testicular function and pelage color are regulated by photoperiod in the Djungarian hamster. To investigate critical daylengths of these functions, adult male hamsters were exposed to one four photoperiods: 16 h light, 8 darkness (16L:8D), 14L:10D, 12L:12D, or 10L:14D. 10L:14D 12L:12D induced winter molt testicular regression, contrast 14L:10D which only latter response, 16L:8D maintained summer large testes. Melatonin injections administered 4, 2, 0 before lights-off mimicked effects...
ABSTRACT During the autumn and winter Djungarian hamsters ( Phodopus sungorus ) exhibit regression of gonads, development a white pelage, loss body weight daily torpor. These seasonal events are largely regulated by changes in photoperiod. The present experiments were designed to examine role testes pineal gland photoperiodically induced torpor loss. Hamsters displayed when exposed short-day photoperiod cold environment, but these phenomena did not occur long days cold. Testicular is...
Abstract The Djungarian hamster exhibits an agouti pelage in the summer and a predominantly white winter. This color cycle is known to be regulated by length of daily photoperiod probably acting through pineal gland, as seasonal reproductive function with which it closely correlated (Figala et al., '73; Hoffmann, '78b). possibility causal relationship between decline gonadal hormone secretion coat change occurring short was examined. Gonadectomized intact male female hamsters were exposed...
The quantity and quality of food intake have been considered crucial for peoples' wellness. Only recently has it become appreciated that the timing is also critical. Nondipping blood pressure (BP) prevalent in diabetic patients associated with increased cardiovascular events. However, causes mechanisms nondipping BP diabetes are not fully understood. Here, we report were arrhythmic db/db mice fed a normal chow diet ad libitum. Imposing diurnal rhythm by time-restricted feeding (TRF; was only...
This study was designed to determine whether the 24-h rhythms of clock gene expression and vascular smooth muscle (VSM) contractile responses are altered in type 2 diabetic db/db mice. Control mice were euthanized at 6-h intervals throughout day. The aorta, mesenteric arteries, heart, kidney, brain isolated. Clock target mRNA levels determined by either real-time PCR or situ hybridization. Isometric contractions measured isolated aortic helical strips, pressor an intravenous injection...
The VicRK 2-component system of Streptococcus mutans regulates genes associated with cell wall biogenesis and biofilm formation. A putative RNase III–encoding gene ( rnc) is located downstream from the vicRKX operon. goals this study were to investigate potential role VicR in regulation adjacent evaluate transcription levels vicR during planktonic growth. Quantitative real-time polymerase chain reaction (qRT-PCR) was used whether cotranscribed. Binding purified recombinant protein promoter...
Accumulation of amyloid-β (Aβ) plays an important role in Alzheimer's disease (AD) pathology. There is growing evidence that disordered sleep may accelerate AD pathology by impeding the physiological clearance Aβ from brain occurs normal sleep. Therapeutic strategies for improving quality therefore help slow progression. It well documented composition and dynamics are sensitive to ambient temperature. We compared metrics derived polysomnography 12-month-old female 3xTg-AD mice (n = 8)...
Carbohydrate mutants were isolated from Rhizobium meliloti L5-30 using the translocatable drug-resistance element Tn5. Enzyme assays with cell-free extracts of four showed that they lacked mannitol dehydrogenase, ribose kinase, xylose isomerase and fructose respectively. An l-arabinose mutant was also isolated. Uptake studies ribose, still utilized sugars on which unable to grow, possibly indicating R. possesses alternative metabolic routes do not result in growth. All able nodulate alfalfa...
Shifting the onset of light, acutely or chronically, can profoundly affect responses to infection, tumor progression, development metabolic disease, and mortality in mammals. To date, majority phase-shifting studies have focused on acute exposure a shift timing light cycle, whereas consequences chronic phase shifts alone molecular rhythms peripheral tissues such as skeletal muscle not been studied. In this study, we tested effect advance clock mechanism two phenotypically different muscles....
Cysteine proteases, including Arg-gingipain of Porphyromonas gingivalis, have been implicated as important virulence factors in periodontal diseases. These enzymes are also involved the hemagglutinating activity organisms. In order to determine role proteases colonization gingival margin, we compared attachment properties P. gingivalis 381 with those its Arg-gingipain-defective mutant, G-102. Interactions gram-positive bacteria, human oral epithelial cells, extracellular matrix proteins, and...