A5055919949- Pluripotent Stem Cells Research
- Construction Project Management and Performance
- Cancer-related gene regulation
- BIM and Construction Integration
- Business Process Modeling and Analysis
- Wnt/β-catenin signaling in development and cancer
- Tissue Engineering and Regenerative Medicine
- Hedgehog Signaling Pathway Studies
- Reading and Literacy Development
- Epigenetics and DNA Methylation
- Neurogenetic and Muscular Disorders Research
- Genetic and Kidney Cyst Diseases
- Reproductive Biology and Fertility
- Obstructive Sleep Apnea Research
- CRISPR and Genetic Engineering
- Parasitic infections in humans and animals
- Renal and related cancers
- dental development and anomalies
- Appendicitis Diagnosis and Management
- Metabolomics and Mass Spectrometry Studies
- Vascular anomalies and interventions
- Cognitive and developmental aspects of mathematical skills
- Mechanical Circulatory Support Devices
- Mesenchymal stem cell research
- Chromosomal and Genetic Variations
Texas A&M University
2017
University of Washington
1990-2012
Howard Hughes Medical Institute
2009-2012
University of Georgia
2004-2007
University of Guelph
2004
Scientific and Technological Research Council of Turkey
2004
Anderson Hospital
1990
Signal transduction pathways play diverse, context-dependent roles in vertebrate development. In studies of human embryonic stem cells (hESCs), conflicting reports claim Wnt/β-catenin signaling promotes either self-renewal or differentiation. We use a sensitive reporter to establish that is not active during hESC self-renewal. Inhibiting this pathway over multiple passages has no detrimental effect on maintenance, whereas activating results loss and induction mesoderm lineage genes....
Chibby (Cby) is a conserved component of the Wnt–β-catenin pathway. Cby physically interacts with β-catenin to repress its activation transcription. To elucidate function in vertebrates, we generated Cby−/− mice and found that after 2–3 d weight loss, majority die before or around weaning. All develop rhinitis sinusitis. When challenged Pseudomonas aeruginosa isolates, are unable clear bacteria from nasal cavity. Notably, exhibit complete absence mucociliary transport caused by marked...
Silvério KG, Davidson KC, James RG, Adams AM, Foster BL, Nociti FH Jr, Somerman MJ, Moon RT. Wnt/β‐catenin pathway regulates bone morphogenetic protein (BMP2)‐mediated differentiation of dental follicle cells. J Periodont Res 2012; 47: 309–319. © 2011 John Wiley & Sons A/S Background and Objective: Bone 2 (BMP2)‐induced osteogenic has been shown to occur through the canonical Wnt/βcatenin pathway, whereas factors promoting Wnt signaling in cementoblasts inhibit cell promote proliferation...
INTRODUCTION The Wnt/β-catenin pathway is the best characterized Wnt largely as a result of powerful assays that allow measurement in vivo and vitro activation. Early for signaling included phenotypic Drosophila , dorsal axis duplication Xenopus proliferation C57MG mammary epithelial cells. More recent characterizations rely on direct culminates with T-cell factor/lymphoid enhancer factor (TCF/LEF)-dependent regulation target gene transcription; assaying such transcription reporters...
Abstract RNA interference (RNAi) has rapidly developed into one of the most widely applied technologies in molecular and cellular research, although young, is now an essential experimental tool. The versatility RNAi, especially mammalian species, lends to its potential applications a wide array fields. Without having genetically manipulate genome, ability selectively reduce level specific transcript using small interfering (siRNA) molecules great appeal studying reprogramming issues somatic...
Human embryonic stem (ES) cells are promising resources for developing new treatments neurodegenerative diseases. Spinal muscular atrophy (SMA) is one of the leading causes childhood paralysis and infant mortality. SMA caused by inactivation survival motor neuron-1 (SMN1) gene. The nearly identical SMN2 gene contains a silent polymorphism that disrupts splicing as result cannot compensate loss SMN1. Project was established National Institute Neurological Disorders Stroke (NINDS) pilot effort...
The ability to produce cloned livestock using postmortem tissue could incorporate an additional application into the field of nuclear transfer. This study examined feasibility producing cattle a primary cell line established from beef carcass. A market heifer processed at USDA-certified slaughterhouse was used develop somatic line. Tissue samples were taken kidney and forelimb regions either 1) immediately following slaughter (fresh) or 2) 48h postslaughter (cooled) where carcass housed 2...
There are many projects using discrete event simulation as a decision-making tool. However, it was found in the literature that these articles related to improving execution of projects, not studying issues management projects. From this perspective, paper used concepts proposed by PMBOK <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">®</sup> drive communication between members project and stakeholders. To achieve goal, real case manufacturing...
A porcine trophoblastic cell line could provide a powerful model for understanding trophoblast biology as well placental gene expression and proteomics in vitro. In this experiment, we derived cells from trophectoderm tissue assessed their growth on three different extracellular matrix substrates concentrations of human recombinant bone morphogenetic protein 4 (hBMP4). Human BMP4 has been shown to induce differentiation embryonic stem into lineages. Elongated embryos were flushed using DPBS...
There are many projects using discrete event simulation as a decision-making tool. However, it was found in the literature that these articles related to improving execution of projects, not studying issues management projects. From this perspective, paper used concepts proposed by PMBOK® drive communication between members project and stakeholders. To achieve goal, real case manufacturing company studied. The steps structure were followed an Action Plan elaborated applied project. One...
A method to determine the cell cycle and growth rate of germinal tissue from Echinococcus multilocularis E. vogeli using flow cytometry is described. In particular, technique involves use either a mercury arc-lamp or laser-equipped system, depending on general availability instruments. Of 5 DNA fluorescent stains tested, only mixture ethidium bromide mithramycin (an energy-transfer method) results in adequate resolution cell-cycle sufficient separation background noise mercury-arc lamp...