A5101723804- RNA modifications and cancer
- RNA Research and Splicing
- Neurogenetic and Muscular Disorders Research
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- interferon and immune responses
- Animal Virus Infections Studies
- HIV Research and Treatment
- Connective tissue disorders research
- Signaling Pathways in Disease
- Cancer Research and Treatments
- Cardiac Structural Anomalies and Repair
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Herpesvirus Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Phytase and its Applications
- Parkinson's Disease Mechanisms and Treatments
- Bone health and treatments
- Genetics and Neurodevelopmental Disorders
- Congenital Anomalies and Fetal Surgery
- Enzyme Production and Characterization
- CRISPR and Genetic Engineering
- Hepatitis B Virus Studies
- RNA Interference and Gene Delivery
Zhengzhou University of Science and Technology
2024-2025
Sun Yat-sen University
2024
Sun Yat-sen University Cancer Center
2024
State Key Laboratory of Oncology in South China
2024
Southern Medical University
2023
Nantong University
2011-2020
Central South University
2004-2019
Xiangya Hospital Central South University
2017-2019
Shanghai Eighth People Hospital
2019
ShenZhen People’s Hospital
2018
Two groups of tau, 3R- and 4R-tau, are generated by alternative splicing tau exon 10. Normal adult human brain expresses equal levels them. Disruption the physiological balance is a common feature several tauopathies. Very early in their life, individuals with Down syndrome (DS) develop Alzheimer-type pathology, molecular basis for which not fully understood. Here, we demonstrate that Dyrk1A, kinase encoded gene DS critical region, phosphorylates factor (ASF) at Ser-227, Ser-234, Ser-238,...
The survival motor neuron (SMN) protein forms cytoplasmic granules when overexpressed. We report here that SMN co‐localizes with TIA‐1/R and G3BP, assemblers of stress (SGs), is co‐immunoprecipitated TIA‐1/R, suggesting are SGs. Formation precedes accumulation indicating serves as a facilitator SG formation. However, the exon 7 skipping product, SMNΔ7, largely retained in nucleus nuclear granules, critical for Our findings reveal novel function possible involvement pathogenesis spinal...
<i>Tau</i> exon 10, which encodes the second microtubule-binding repeat, is regulated by alternative splicing. Its splicing generates Tau isoforms with three- or four-microtubule-binding repeats, named 3R-tau and 4R-tau. Adult human brain expresses equal levels of Imbalance 4R-tau causes aggregation neurofibrillary degeneration. In present study, we found that factor SRp55 (serine/arginine-rich protein 55) promoted 10 inclusion. Knockdown significantly exclusion. The promotion inclusion...
Significance Here, we present data showing that monocyte chemotactic protein-induced protein 1 (MCPIP1) acts as an RNase to limit HIV-1 production in resting CD4+ T cells. Unlike those previously identified factors with restrictions tend be overcome by virally encoded proteins, MCPIP1 becomes rapidly degraded on activation of human These findings provide insights into the mechanisms cellular activation-mediated cells and represent a breakthrough relevant field.
Significance Transcription is a biological procedure in which DNA transcribed to an RNA molecule. However, only fragments of this are needed for protein synthesis. These exons that interrupted by introns. Introns removed so-called splicing process. Some could be alternatively included or excluded from the final In study, we have found U2 snRNP auxiliary factor 65 kDa (U2AF ), general regulator, can significantly promote exclusion alternative exons. Strikingly, U2AF suppresses flanking intron...
Epstein-Barr virus (EBV)-positive diffuse large B-cell lymphoma (EBV+ DLBCL) is a rare type of with high incidence in elderly patients, poor drug response, and unfavorable prognosis. Despite advances genomic profiling precision medicine DLBCL, EBV+ DLBCL remain poorly characterized understood. We include 236 patients for EBV-encoded mRNA (EBER) situ hybridization detection analyzed 9 6 EBV negative cases by next-generation sequencing (NGS). then performed fluorescence (FISH)...
Gliomas are the most frequently occurring malignant brain cancers. Recently, isocitrate dehydrogenase (IDH) mutations, O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation, and 1p/19q co-deletion have been suggested to indicate a favorable prognosis in gliomas. However, clinical prognostic value of these genetic tests human gliomas is not fully understood.We included glioma patients who accepted testing including IDH, MGMT at Xiangya Hospital, Central South University China...
Wound healing involves an orchestrated response that engages multiple processes such as hemostasis, cellular migration, extracellular matrix synthesis, and in particular, inflammation. Using a murine model of cutaneous wound repair, the transcriptome was mapped from 12 hours to 8 days post-injury, multicomponent, multi-target natural product, Tr14. single-molecule RNA sequencing (RNAseq), there were clear temporal changes known transcripts related pathways, additional novel both coding...
Abstract Mutations that stimulate exon 10 inclusion into the human tau mRNA cause frontotemporal dementia with parkinsonism, associated chromosome 17 (FTDP‐17), and other tauopathies. This suggests ratio of to exclusion in adult brain is one factors determine biological functions protein. To investigate underlying splicing mechanism identify potential therapeutic targets for tauopathies, we generated a series mini‐gene constructs intron deletions from full length exons 9–11 construct. RT–PCR...
Frontotemporal dementia with parkinsonism linked to chromosome 17 (FTDP-17) is caused by mutations in the gene encoding microtubule-associated protein, tau. Some FTDP-17 affect exon 10 splicing. To correct aberrant splicing while retaining endogenous transcriptional control, we evaluated feasibility of using spliceosome-mediated RNA trans-splicing (SMaRT) reprogram tau mRNA. We designed a pre-trans-splicing molecule containing human exons 13 and binding domain complementary 3′ end intron 9....
Spinal muscular atrophy is an autosomal recessive motor neuron degenerative disorder, caused by the loss of telomeric copy survival gene (SMN1). To better understand how neurons are targeted in patients, it important to study role SMN protein cell death. In this report, we employed RNA interference (RNAi) loss-of-function Drosophila S2 cells. A 601-base pair double-stranded (dsRNA) (dSMN) was used for silencing dSMN. Our data indicate that dSMN RNAi resulted more than 90% reduction both and...
Human cells are known to express many chimeric RNAs, i.e. RNAs containing two genes' sequences. Wondering whether there also is trimeric RNA, an RNA three sequences, we wrote simple computer code screen human expression sequence tags (ESTs) deposited in different public databases, and obtained hundreds of putative ESTs. We then used NCBI Blast UCSC Blat browsers further analyze their identified 61 tetrameric ESTs (one EST four sequences). 57 chimeric, or teterameric that contained both...