A5032751779- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Neurogenetic and Muscular Disorders Research
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Celiac Disease Research and Management
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Systemic Lupus Erythematosus Research
- Liver physiology and pathology
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Rheumatoid Arthritis Research and Therapies
- Proteoglycans and glycosaminoglycans research
- Cancer Research and Treatments
- Macrophage Migration Inhibitory Factor
- Microscopic Colitis
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Whipple's Disease and Interleukins
- RNA regulation and disease
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Protein Tyrosine Phosphatases
Monash University
2021-2024
Australian Regenerative Medicine Institute
2021-2024
Gwangju Institute of Science and Technology
2012-2017
Significance Transcription is a biological procedure in which DNA transcribed to an RNA molecule. However, only fragments of this are needed for protein synthesis. These exons that interrupted by introns. Introns removed so-called splicing process. Some could be alternatively included or excluded from the final In study, we have found U2 snRNP auxiliary factor 65 kDa (U2AF ), general regulator, can significantly promote exclusion alternative exons. Strikingly, U2AF suppresses flanking intron...
Citrullination of a fibrinogen peptide influences HLA-DR4 binding and TCR usage by neoepitope-reactive T cells in rheumatoid arthritis.
Abstract CD4 + T cells recognising citrullinated self-epitopes presented by HLA-DRB1 bearing the shared susceptibility epitope (SE) are implicated in rheumatoid arthritis (RA). However, underlying cell receptor (TCR) determinants of specificity towards distinct peptide antigens, including vimentin-64cit 59-71 and α-enolase-15cit 10-22 remain unclear. Using HLA-DR4-tetramers, we examine repertoire HLA-DR4 transgenic mice observe biased TRAV6 TCR gene usage across these two epitopes which...
SRSF2, a Serine-Arginine rich (SR) protein, is splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron to suppress cassette in SMN premRNA. Through serial mutagenesis, demonstrate 10 nt RNA sequence surrounding the branch-point (BP), important for SRSF2-mediated inhibition of through interacting with SRSF2. We conclude inhibits promote exclusion. [BMB Reports 2017; 50(8): 423-428].
CD44 pre-mRNA includes 20 exons, of which exons 1-5 (C1-C5) and 16-20 (C6-C10) are constant whereas 6-15 (V1-V10) variant exons. V6-exon-containing isoforms have been known to be implicated in tumor cell invasion metastasis. In the present study, we performed a SR protein screen for V6 splicing using overexpression lentivirus-mediated shRNA treatment. Using minigene, demonstrate that increased SRSF3 SRSF4 expression do not affect splicing, but SRSF1, SRSF6 SRSF9 significantly inhibit...
The récepteur d'origine nantais (RON) gene is a proto-oncogene that responsible for encoding the human macrophage-stimulating protein (MSP) 1 receptor. MSP activation induces RON-mediated cell dissociation, migration and matrix invasion. Isoforms of RON exclude exons 5 6 encode RONΔ160 protein, which promotes transformation in vitro tumor metastasis vivo. Premature termination codons (PTCs) activate nonsense-mediated mRNA decay (NMD) signaling pathway. present study demonstrated PTCs at...
The mouse immunoglobulin (IgM) pre-mRNA contains a splicing inhibitor that bears multiple binding sites for the repressor polypyrimidine tract protein (PTB). Here we show directs assembly of an ATP-dependent complex PTB and U1 U2 small nuclear RNAs (snRNAs). Unexpectedly, although snRNA is present in complex, it not base-paired to branch point. We evidence inhibitor-bound contacts promote base-pairing adjacent point–like sequence within inhibitor, thereby preventing snRNA–branch point...
Abstract As part of the Monash Sensory Science Exhibition, our team guided participants through a multisensory journey unraveling coeliac disease development and pathology. Through tactile sensory exhibits, we showed how benign dietary gluten can be transformed into harmful entity for 1 in 70 Australians with this illness. In contrast to common misconception as food allergy, exhibits revealed its closer association autoimmune diseases such type diabetes, involving genetic susceptibility...