A5057188505- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Neurogenetic and Muscular Disorders Research
- Cancer-related molecular mechanisms research
- Liver physiology and pathology
- Cancer-related gene regulation
- Galectins and Cancer Biology
- Cytokine Signaling Pathways and Interactions
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Mycobacterium research and diagnosis
- Proteoglycans and glycosaminoglycans research
- Genetic factors in colorectal cancer
- Protein Degradation and Inhibitors
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- Protein Tyrosine Phosphatases
- Cancer Research and Treatments
- Macrophage Migration Inhibitory Factor
- Biotin and Related Studies
- Cancer Mechanisms and Therapy
Daegu-Gyeongbuk Medical Innovation Foundation
2021-2025
Gwangju Institute of Science and Technology
2012-2019
Significance Transcription is a biological procedure in which DNA transcribed to an RNA molecule. However, only fragments of this are needed for protein synthesis. These exons that interrupted by introns. Introns removed so-called splicing process. Some could be alternatively included or excluded from the final In study, we have found U2 snRNP auxiliary factor 65 kDa (U2AF ), general regulator, can significantly promote exclusion alternative exons. Strikingly, U2AF suppresses flanking intron...
Janus kinase 1 (JAK1) plays a key role in most cytokine-mediated inflammatory and autoimmune responses through JAK/STAT signaling; thus, JAK1 inhibition is promising therapeutic strategy for several diseases. Analysis of the binding modes current JAK inhibitors to isoforms allowed design N-alkyl-substituted 1-H-pyrrolo[2,3-b] pyridine carboxamide as JAK1-selective scaffold, synthesis various methyl amide derivatives provided...
SRSF2, a Serine-Arginine rich (SR) protein, is splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron to suppress cassette in SMN premRNA. Through serial mutagenesis, demonstrate 10 nt RNA sequence surrounding the branch-point (BP), important for SRSF2-mediated inhibition of through interacting with SRSF2. We conclude inhibits promote exclusion. [BMB Reports 2017; 50(8): 423-428].
CD44 pre-mRNA includes 20 exons, of which exons 1-5 (C1-C5) and 16-20 (C6-C10) are constant whereas 6-15 (V1-V10) variant exons. V6-exon-containing isoforms have been known to be implicated in tumor cell invasion metastasis. In the present study, we performed a SR protein screen for V6 splicing using overexpression lentivirus-mediated shRNA treatment. Using minigene, demonstrate that increased SRSF3 SRSF4 expression do not affect splicing, but SRSF1, SRSF6 SRSF9 significantly inhibit...
Here we show that the serine/arginine rich splicing factor 2 (SRSF2) promotes cryptic 3′ splice-site (3′AG′) usage during cassette exon exclusion in survival of motor neuron (SMN2) minigenes. Deletion 3′AG′ (3′AG′1), its associated branch point (BP′) and polypyrimidine tract (PPT′) sequences directs SRSF2 to promote a second (3′AG′2) with less conserved region for intron splicing. Furthermore, deletion both 3′AG′1 3′AG′2 their triggered third 3′AG′3 has very weak sequences. Interestingly,...
The récepteur d'origine nantais (RON) gene is a proto-oncogene that responsible for encoding the human macrophage-stimulating protein (MSP) 1 receptor. MSP activation induces RON-mediated cell dissociation, migration and matrix invasion. Isoforms of RON exclude exons 5 6 encode RONΔ160 protein, which promotes transformation in vitro tumor metastasis vivo. Premature termination codons (PTCs) activate nonsense-mediated mRNA decay (NMD) signaling pathway. present study demonstrated PTCs at...
The mouse immunoglobulin (IgM) pre-mRNA contains a splicing inhibitor that bears multiple binding sites for the repressor polypyrimidine tract protein (PTB). Here we show directs assembly of an ATP-dependent complex PTB and U1 U2 small nuclear RNAs (snRNAs). Unexpectedly, although snRNA is present in complex, it not base-paired to branch point. We evidence inhibitor-bound contacts promote base-pairing adjacent point–like sequence within inhibitor, thereby preventing snRNA–branch point...
Other SectionsABSTRACTINTRODUCTIONRESULTSDISCUSSIONMATERIALS AND METHODSACKNOWLEDGEMENTSCONFLICTS OF INTERESTFIGURESREFERENCES
RON receptor tyrosine kinase is a proto-oncogene that induces cell migration and matrix invasion.RONΔ160 protein, which produced by exclusion of exon 5 6, promotes migration, invasion protection from apoptosis.Alternative splicing regulation 6 not well understood.In this manuscript, we identified several new RNA regulatory elements for alternative Ron proto-oncogene.Firstly, demonstrated sequences EcoRI cleavage sites regulate 6.Secondly, showed the ~30 nt at upstream end 4 ~33 downstream 7...