A5058248427- RNA Research and Splicing
- RNA modifications and cancer
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Infectious Diseases and Mycology
- Lipid metabolism and biosynthesis
- interferon and immune responses
- Neurogenetic and Muscular Disorders Research
- Cytomegalovirus and herpesvirus research
- Receptor Mechanisms and Signaling
- MicroRNA in disease regulation
- Viral-associated cancers and disorders
- RNA regulation and disease
- Down syndrome and intellectual disability research
- Metabolism, Diabetes, and Cancer
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Circular RNAs in diseases
- Animal Virus Infections Studies
- Protein Structure and Dynamics
- Pediatric health and respiratory diseases
- Cancer-related gene regulation
- Regional Development and Environment
Korea Research Institute of Bioscience and Biotechnology
2016-2025
Konkuk University
2023-2024
Korea University of Science and Technology
2014-2024
Korea University
2002-2022
University of Science and Technology
2013-2014
Universidad Mayor
2013
University of Pennsylvania
2008-2012
Howard Hughes Medical Institute
2010-2012
Pohang University of Science and Technology
2000-2011
National Renewable Energy Laboratory
2009
Spinal muscular atrophy (SMA) is caused by homozygous survival of motor neurons 1 ( SMN1 ) gene deletions, leaving a duplicate gene, SMN2 , as the sole source SMN protein. However, most mRNA produced from pre-mRNA exon 7-skipped (∼80%), resulting in highly unstable and almost undetectable protein (SMNΔ7). We show that this splicing defect creates potent degradation signal (degron; SMNΔ7-DEG) at SMNΔ7's C-terminal 15 amino acids. The S270A mutation inactivates SMNΔ7-DEG, generating stable...
ABSTRACT The translation of polioviral mRNA occurs through an internal ribosomal entry site (IRES). Several RNA-binding proteins, such as polypyrimidine tract-binding protein (PTB) and poly(rC)-binding (PCBP), are required for the poliovirus IRES-dependent translation. Here we report that a protein, 3C pro (and/or 3CD ), cleaves PTB isoforms (PTB1, PTB2, PTB4). Three target sites (one major two minor sites) exist in PTBs. fragments generated by infection redistributed to cytoplasm from...
Alternative splicing is a nearly ubiquitous versatile process that controls gene expression and creates numerous protein isoforms with different functions from single gene. The significance of alternative has been confirmed by the increasing number human diseases are caused misregulation events. Very few compounds, however, have reported to act as inhibitors splicing, their potential clinical use needs be evaluated. Here, we report CX-4945, previously well-characterized inhibitor casein...
G-protein coupled receptor 43 (GPR43) serves as a for short-chain fatty acids (SCFAs), implicated in neutrophil migration and inflammatory cytokine production. However, the intracellular signaling pathway mediating GPR43 remains unclear. Here, we show that β-arrestin 2 mediates internalization of by agonist. Agonism reduced phosphorylation nuclear translocation factor-κB (NF-κB), which was relieved short interfering RNA (siRNA) 2. Subsequently, mRNA expression proinflammatory cytokines,...
DYRK1A is important in neuronal development and function, its excessive activity considered a significant pathogenic factor Down syndrome Alzheimer's disease. Thus, inhibition of has been suggested to be new strategy modify the Very few compounds, however, have reported act as inhibitors, their potential clinical uses require further evaluation. Here, we newly identify CX-4945, safety which already proven setting, potent inhibitor that acts an ATP-competitive manner. The inhibitory potency...
The circadian rhythm of pineal melatonin requires the nocturnal increment serotonin N -acetyltransferase (arylalkylamine [AANAT]) protein. To date, only limited information is available in critical issue how AANAT protein expression up-regulated exclusively at night regardless its species-specific mRNA profiles. Here we show that timing regulated by rhythmic translation mRNA. This control mediated both a highly conserved IRES (internal ribosome entry site) element within 5′ untranslated...
Translational initiation of hepatitis C virus (HCV) mRNA occurs by internal entry ribosomes into an ribosomal site (IRES) at the 5′ nontranslated region. A region encoding N-terminal part HCV polyprotein has been shown to augment translation mRNA. Here we show that a cellular protein, NS1-associated protein 1 (NSAP1), augments through specific interaction with adenosine-rich protein-coding within The overexpression NSAP1 specifically enhanced IRES-dependent translation, and knockdown use...
Cordyceps species are known to produce numerous active components and used for diverse medicinal purposes because of their varied physiological activities, including ability protect the liver from damage as well anticancer, antidepressant, anti-inflammatory, hypoglycemic, antimicrobial effects.Cordycepin, an adenosine derivative, differs in that its ribose lacks oxygen atom at 3′ position.Several research groups have reported cordycepin has antiviral activity against several viruses...
Ginsenosides are the major components responsible for biochemical and pharmacological actions of ginseng, have been shown to various biological activities. In this study, we investigated antiviral activities seven ginsenosides [protopanaxatriol (PT) type: Re, Rf, Rg2; protopanaxadiol (PD) Rb1, Rb2, Rc, Rd)] against coxsackievirus B3 (CVB3), enterovirus 71 (EV71), human rhinovirus 3 (HRV3). Assays activity cytotoxicity were evaluated by sulforhodamine B method using cytopathic effect (CPE)...
Pooled CRISPR screens based on lentiviral systems have been widely applied to identify the effect of gene knockout cellular phenotype. Although many were successful, they also limitation that genes conferring mild phenotypes or those essential for growth can be overlooked, as every genetic perturbation is incorporated in same population. Arrayed screens, other hand, incorporate a single each well and could overcome these limitations. However, arrayed siRNA-mediated knockdown recently...
Abstract Hepatitis C virus (HCV) is the major causative agent of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma, can be involved in very long infections up to 30 years or more. Therefore, it has been speculated that HCV possesses mechanisms capable modulating host defense systems such as innate adaptive immunity. To investigate this virus-host interaction, we generated replicons containing various structural proteins then analyzed sensitivity replicon-containing cells...
The p27Kip1 protein plays a critical role in the regulation of cell proliferation through inhibition cyclin-dependent kinase activity. Translation is directed by an internal ribosomal entry site (IRES) 5′ nontranslated region mRNA. Here, we report that polypyrimidine tract-binding (PTB) specifically enhances IRES activity mRNA interaction with element. We found addition PTB to vitro translation system and overexpression 293T cells augmented but knockdown introduction PTB-specific small...
Triacylglycerol (TG) is the major form of stored energy in eukaryotic organisms and synthesized by diacylglycerol acyltransferase (DGAT) endoplasmic reticulum (ER). DGAT2, one two DGAT enzymes, barely detectable cells, even though its mRNA transcripts are maintained at considerable levels. However, little known about how DGAT2 expression altered protein stability. was highly unstable cells rapidly degraded proteasomes an ubiquitin-dependent manner. Deletion mutation analysis identified...
Gemcitabine, an anti-cancer chemotherapy drug, has additionally shown the antiviral activity against a broad range of viruses and we also have previously reported its synergistic with ribavirin enteroviruses. As cytidine analog, gemcitabine been to inhibitory on pyrimidine biosynthesis. In addition, few inhibitors biosynthesis induce innate immunity in yet-to-be-determined manner inhibit virus infection. Thus, investigated whether anti-enteroviral is mediated by immunity, induction which...
Human rhinovirus (HRV) causes the common cold and exacerbates chronic respiratory diseases, such as asthma obstructive pulmonary disease. Despite its significant impact on public health, there are currently no approved vaccines or antiviral treatments for HRV infection. Apoptosis is process through which cells eliminate themselves systematic activation of intrinsic death pathways in response to various stimuli. It plays an important role viral infections serves a key immune defense mechanism...
Diacylglycerol acyltransferase 2 (DGAT2) is one of two distinct DGAT enzymes that catalyze the last step in triacylglycerol (TG) synthesis. Findings from previous studies suggest inhibition DGAT2 a promising strategy for treatment hepatic steatosis and insulin resistance. Here, we identified compound 122 as potent selective inhibitor human DGAT2, which appeared to act competitively against oleoyl-CoA vitro. The was also confirmed by reductions enzymatic activity de novo TG synthesis...
Chrysin is a 5,7-dihydroxyflavone and was recently shown to potently inhibit enterovirus 71 (EV71) by suppressing viral 3C protease (3C(pro)) activity. In the current study, we investigated whether chrysin also shows antiviral activity against coxsackievirus B3 (CVB3), which belongs same genus (Enterovirus) as EV71, assessed its ability prevent resulting acute pancreatitis myocarditis. We found that showed CVB3 at 10 μM, but exhibited mild cellular cytotoxicity 50 prompting us synthesize...