A5028509727- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Viral Infections and Immunology Research
- RNA regulation and disease
- Ubiquitin and proteasome pathways
- Hepatitis C virus research
- Nuclear Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Orthopedic Surgery and Rehabilitation
- Cancer-related gene regulation
- Circular RNAs in diseases
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Elbow and Forearm Trauma Treatment
- Autophagy in Disease and Therapy
- HVDC Systems and Fault Protection
- Cancer-related Molecular Pathways
- Viral Infectious Diseases and Gene Expression in Insects
- interferon and immune responses
- Heat shock proteins research
- Genomics and Chromatin Dynamics
- PI3K/AKT/mTOR signaling in cancer
- Bacterial Genetics and Biotechnology
- Cancer-related molecular mechanisms research
Korea Advanced Institute of Science and Technology
2022-2025
Chungnam National University Hospital
2019-2024
Sogang University
2023-2024
Korea University
2013-2022
University of Maryland Medical Center
2020-2021
University of Maryland, Baltimore
2020-2021
Pusan National University
2015
Kangwon National University
2012
Pohang University of Science and Technology
1998-2010
Kwangwoon University
2009
Abstract LC3/ATG8 has long been appreciated to play a central role in autophagy, by which variety of cytoplasmic materials are delivered lysosomes and eventually degraded. However, information on the molecular functions LC3 RNA biology is very limited. Here, we show that LC3B an RNA-binding protein directly binds mRNAs with preference for consensus AAUAAA motif corresponding polyadenylation sequence. Autophagic activation promotes association between target triggers rapid degradation...
Sequence-based analyses have predicted that approximately 35% of mammalian alternative splicing (AS) events produce premature termination codon (PTC)-containing splice variants are targeted by the process nonsense-mediated mRNA decay (NMD). This led to speculation AS may often regulate gene expression activating NMD. Using microarrays, we show PTC-containing generally produced at uniformly low levels across diverse cells and tissues, independently action Our results suggest most...
Alternative splicing (AS) can regulate gene expression by introducing premature termination codons (PTCs) into spliced mRNA that subsequently elicit transcript degradation the nonsense-mediated decay (NMD) pathway. However, range of cellular functions controlled this process and factors required are poorly understood. By quantitative AS microarray profiling, we find there significant overlaps among sets PTC-introducing events affected individual knockdown three core human NMD factors,...
UPF1 functions in both Staufen 1 (STAU1)-mediated mRNA decay (SMD) and nonsense-mediated (NMD), which we show here are competitive pathways. STAU1- UPF2-binding sites within overlap so that STAU1 UPF2 binding to appear be mutually exclusive. Furthermore, down-regulating the cellular abundance of STAU1, inhibits SMD, increases efficiency NMD, whereas UPF2, SMD. Competition under physiological conditions is exemplified during differentiation C2C12 myoblasts myotubes: The SMD NMD decreases,...
COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on human genome remain unexplored. Here, we report a high-resolution atlas transcriptome for various time points after infecting cells. Intriguingly, substantial amount translation initiates at novel initiation site (TIS) located leader sequence, termed TIS-L. Since TIS-L...
Argonaute is the primary mediator of metazoan miRNA targeting (MT). Among currently identified >1,500 human RNA-binding proteins (RBPs), there are only a handful RBPs known to enhance MT and several others reported suppress MT, leaving global impact on elusive. In this study, we have systematically analyzed transcriptome-wide binding sites for 150 evaluated quantitative effect individual efficacy. contrast previous studies, show that most significantly affect all those MT-regulating function...
N6-methyladenosine (m6A) is the most prevalent internal modification in eukaryotic mRNAs and affects RNA processing metabolism. When YTHDF2, an m6A-recognizing protein, binds to m6A, it facilitates destabilization of m6A-containing RNAs (m6A RNAs). Here, we demonstrate that upstream frameshift 1 (UPF1), a key factor for nonsense-mediated mRNA decay, interacts with thereby triggering rapid degradation m6A RNAs. The UPF1-mediated depends on specific interaction between UPF1 N-terminal residues...
Translation initiation of hepatitis C virus (HCV) RNA occurs by internal entry a ribosome into the 5' nontranslated region in cap-independent manner. The HCV sequence from about nucleotide 40 up to N terminus coding core protein is required for efficient translation, though precise border ribosomal site (IRES) has yet be determined. Several cellular proteins have been proposed direct IRES-dependent translation binding IRES. Here we report on novel that specifically interacts with 3' IRES...
The 78-kDa glucose-regulated protein GRP78 is a stress-inducible ubiquitously expressed in animal cells. In this paper we show that the first exon of endoplasmic reticulum-localized consists an 18 amino acid leader sequence rich hydrophobic residues, followed by highly acidic mature N-terminus and 11 domain shared members 70-kDa heat shock family. end also marks beginning intron gene. A DNA region upstream promoter element important for induction calcium ionophore temperature-sensitive...
ABSTRACT The translation of polioviral mRNA occurs through an internal ribosomal entry site (IRES). Several RNA-binding proteins, such as polypyrimidine tract-binding protein (PTB) and poly(rC)-binding (PCBP), are required for the poliovirus IRES-dependent translation. Here we report that a protein, 3C pro (and/or 3CD ), cleaves PTB isoforms (PTB1, PTB2, PTB4). Three target sites (one major two minor sites) exist in PTBs. fragments generated by infection redistributed to cytoplasm from...
During or right after mRNA export via the nuclear pore complex (NPC) in mammalian cells, mRNAs undergo translation mediated by cap-binding proteins 80 and 20 (CBP80/20). After CBP80/20-dependent translation, CBP80/20 is replaced cytoplasmic protein eIF4E, which directs steady-state translation. Nonsense-mediated decay (NMD), one of best-characterized surveillance mechanisms, has been shown to occur on CBP80/20-bound mRNAs. However, despite tight link between NMD, underlying molecular...