A5100712424- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- Cardiac Ischemia and Reperfusion
- Adipose Tissue and Metabolism
- Angiogenesis and VEGF in Cancer
- interferon and immune responses
- Mesenchymal stem cell research
- Metabolism, Diabetes, and Cancer
- Cell Adhesion Molecules Research
- Cardiac Valve Diseases and Treatments
- Autophagy in Disease and Therapy
- Corneal Surgery and Treatments
- Genetic Neurodegenerative Diseases
- Wound Healing and Treatments
- MicroRNA in disease regulation
- Organ Transplantation Techniques and Outcomes
- Cell death mechanisms and regulation
- Cellular Mechanics and Interactions
- Immune Response and Inflammation
- Neurogenetic and Muscular Disorders Research
- Nuclear Structure and Function
- Amyotrophic Lateral Sclerosis Research
- Ubiquitin and proteasome pathways
- Trace Elements in Health
- RNA Interference and Gene Delivery
People's Hospital of Kaizhou District
2024-2025
University of Virginia
2024
The Ohio State University
2014-2023
TRIM edicine (United States)
2012-2023
Lung Institute
2014-2022
The Ohio State University Wexner Medical Center
2014-2022
Columbus Oncology and Hematology Associates
2021
University of Tübingen
2016
Tongji University
2015
Sutter Davis Hospital
2014
Recombinant human MG53 protein can increase membrane repair after injury in cells and reduce pathology animal models of muscle muscular dystrophy.
Irisin protects against ischemia/reperfusion injury to the lung by improving mitochondrial function.
Recombinant MG53 translocates to sites of injury in the proximal tubule kidney and protects mice from acute induced by ischemia or drugs.
Abstract: Irisin, a muscle-origin protein derived from the extracellular domain of fibronectin domain-containing 5 (FNDC5), has been shown to modulate mitochondria welfare through paracrine action. Here, we test hypothesis that irisin contributes cardioprotection after myocardial infarction by preserving mitochondrial function in cardiomyocytes. Animal model studies show intravenous administration exogenous produces dose-dependent protection against ischemia/reperfusion (I/R)-induced injury...
MG53 is a muscle-specific TRIM-family protein that presides over the cell membrane repair response. Here, we show present in blood circulation acts as myokine to facilitate tissue injury-repair and regeneration. Transgenic mice with sustained elevation of bloodstream (tPA-MG53) have healthier longer life-span when compared littermate wild type mice. The tPA-MG53 normal glucose handling insulin signaling skeletal muscle, does not deleterious impact on db/db More importantly, display...
Cancer cells develop resistance to chemotherapeutic intervention by excessive formation of stress granules (SGs), which are modulated an oncogenic protein G3BP2. Selective control G3BP2/SG signaling is a potential means treat non-small cell lung cancer (NSCLC).Co-immunoprecipitation was conducted identify the interaction MG53 and Immunohistochemistry live imaging were performed visualize subcellular expression or co-localization. We used shRNA knock-down G3BP2 test migration colony...
Ischemic injury to the heart induces mitochondrial dysfunction due increasing oxidative stress. MG53, also known as TRIM72, is highly expressed in striated muscle, secreted a myokine after exercise, and essential for repairing damaged plasma membrane of many tissues by interacting with lipid phosphatidylserine (PS). We hypothesized MG53 could preserve integrity an ischemic event binding mitochondrial-specific lipid, cardiolipin (CL), mitochondria protection prevent mitophagy. Fluorescent...
Ischemic injury to neurons represents the underlying cause of stroke brain. Our previous studies identified MG53 as an essential component cell membrane repair machinery. Here we show that recombinant human (rh)MG53 protein facilitates ischemia-reperfusion (IR) rapidly moves acute sites on neuronal cells form a patch. IR-induced brain increases permeability blood-brain-barrier, providing access from blood circulation target injured tissues. Exogenous rhMG53 can protect cultured against...
Abstract Mesenchymal stem cell‐based therapy has emerged as a promising approach for the treatment of peripheral arterial disease. The purpose this study was to examine potential effects human placenta‐derived mesenchymal cells ( PMSC s) on mouse hindlimb ischemia. s were isolated from placenta tissue and characterized by flow cytometry. An in vivo surgical ligation‐induced murine limb ischemia model generated with fluorescent dye CM ‐DiI) labelled delivered via intramuscular injection. Our...
Traumatic brain injury (TBI) is a common neurotrauma leading to dysfunction and death. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) hold promise in the treatment of TBI. However, their efficacy modest due low survival differentiation under harsh microenvironment injured brain. MG53, member TRIM family protein, plays vital role cell tissue damage repair. The present study aims test whether MG53 preserves hUC-MSCs against oxidative stress enhances TBI treatment.In this study,...
MG53 is a member of the TRIM protein family that predominantly expressed in striated muscles and participates cell membrane repair. Controversy exists regarding MG53’s role insulin signaling manifestation diabetes. We generated db/db mice with either whole-body ablation or sustained elevation bloodstream order to evaluate physiological function To quantify amount circulation, we developed monoclonal antibody against high specificity. Western blot using this revealed lower no change serum...
As a de-ubiquitin enzyme, ubiquitin C-terminal hydrolase (UCH)-L1 has been shown to be overexpressed in several human cancers. However, the function of UCH-L1 invasion breast cancers is still unclear. Here we report that expression significantly higher cancer cells with invasive ability. While ectopic failed alter cell proliferation MCF-7 cells, it caused significant upregulation cellular invasion. Furthermore, siRNA mediated knockdown led suppression overexpressing cells. In order identify...
The cornea plays an important role in transmitting light and providing protection to the eye, but is susceptible injury infection. Standard treatments for corneal wounds include topical lubricants, antibiotics, bandage contact lens, surgery. However, these measures are often ineffective. Here we show that MG53, a protein with essential cell membrane repair, contributes injury-repair process. Native MG53 present epithelia, tear film, aqueous humor, suggesting its potential function...
Aging is associated with chronic oxidative stress and inflammation that affect tissue repair regeneration capacity. MG53 a TRIM family protein facilitates of cell membrane injury in redox-dependent manner. Here, we demonstrate the expression was reduced failing human hearts aged mouse hearts, concomitant elevated NF-κB activation. We evaluated safety efficacy longitudinal, systemic administration recombinant (rhMG53) mice. Echocardiography pressure-volume loop measurements revealed...
Metabolic syndrome is a cluster of risk factors, such as obesity, insulin resistance, and hyperlipidemia that increases the individual's likelihood developing cardiovascular diseases. Patients inflicted with metabolic disorders also suffer from tissue repair defect. Mitsugumin 53 (MG53) protein essential to cellular membrane repair. It facilitates nucleation intracellular vesicles sites disruption create patches, contributing regenerative capacity skeletal cardiac muscle tissues upon injury....
Abstract Cell therapy with bone marrow stem cells ( BMSC s) remains a viable option for tissue repair and regeneration. A major challenge cell is the limited survival after implantation. This study was to investigate effect of oxidized low‐density lipoprotein (ox‐ LDL , naturally present in human blood) on injury MG 53, protein, improvement survival. Rat multipotent adult progenitor MAPC were treated ox‐ which caused significant death as reflected by increased LDH release media. Exposure s...
Zinc is an essential trace element that participates in a wide range of biological functions, including wound healing. Although Zn2+ deficiency has been linked to compromised healing and tissue repair human diseases, the molecular mechanisms underlying Zn2+-mediated remain unknown. Our previous studies established MG53, TRIM (tripartite motif) family protein, component cell membrane machinery. Domain homology analysis revealed MG53 contains two Zn2+-binding motifs. Here, we show binding...
ABSTRACT Introduction : Ischemia‐reperfusion injury (I‐R) in skeletal muscle requires timely treatment. Methods Rodent models of I‐R were used to test the efficacy recombinant human MG53 (rhMG53) protein for protecting muscle. Results In a mouse model, we found that mg53,−/− mice are more susceptible injury. rhMG53 applied intravenously wild‐type protected injured muscle, as demonstrated by reduced CK release and Evans blue staining. Histochemical studies confirmed beneficial effects rhMG53....
// Junmou Hong 1,* , Zhenguo Liu Hua Zhu 2 Xin Zhang 1 Yongju Liang 3 Shiyuan Yao Fang Wang Xiaoyun Xie 4 Bo Tao Tan Liwu Fu Jing Nie 5 and Chao Cheng Department of Thoracic Surgery, The First Affiliated Hospital Sun Yat-sen University, Guangzhou, People's Republic China Davis Heart Lung Research Institute, Ohio State University Wexner Medical Center, Columbus, OH, USA Key Laboratory Oncology in South China, Cancer Division Geriatrics, Tongji Hospital, School Medicine, Shanghai, Nephrology,...