A5084486486- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA Research and Splicing
- RNA modifications and cancer
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
- Genomics and Phylogenetic Studies
- Pharmacogenetics and Drug Metabolism
- RNA Interference and Gene Delivery
- Invertebrate Immune Response Mechanisms
- Circular RNAs in diseases
- Insect symbiosis and bacterial influences
- Cancer Genomics and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Mosquito-borne diseases and control
- Carcinogens and Genotoxicity Assessment
- Genetic and phenotypic traits in livestock
- Single-cell and spatial transcriptomics
- Radiation Dose and Imaging
- Neurobiology and Insect Physiology Research
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Effects of Radiation Exposure
Hanyang University
2016-2025
Anyang University
2016-2023
University of Washington
2023
Korea Research Institute of Bioscience and Biotechnology
2023
Whitehead Institute for Biomedical Research
2010-2015
Massachusetts Institute of Technology
2010-2015
Howard Hughes Medical Institute
2010-2015
Seoul National University
2006-2009
Genomics (United Kingdom)
2006
Life University
2005
MicroRNA targets are often recognized through pairing between the miRNA seed region and complementary sites within target mRNAs, but not all of these canonical equally effective, both computational in vivo UV-crosslinking approaches suggest that many mRNAs targeted non-canonical interactions. Here, we show recently reported do mediate repression despite binding miRNA, which indicates vast majority functional canonical. Accordingly, developed an improved quantitative model targeting, using a...
blood cells, called hemocytes, are classified into plasmatocytes, crystal and lamellocytes based on the expression of a few marker genes cell morphologies, which inadequate to classify complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes states involved cycle, antimicrobial response, metabolism together with identification intermediate states. Further, discovered rare...
Abstract The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis immunity. Three types hemocytes have characterized in gland: plasmatocytes, lamellocytes, crystal cells, which are analogous to vertebrate myeloid yet molecular underpinnings gland less investigated. Here, we use single-cell RNA sequencing comprehensively analyze heterogeneity developing discover...
Abstract Background MicroRNAs (miRNAs) are small noncoding RNAs, which play significant roles as posttranscriptional regulators. The functions of animal miRNAs generally based on complementarity for their 5' components. Although several computational miRNA target-gene prediction methods have been proposed, they still limitations in revealing actual target genes. Results We implemented miTarget, a support vector machine (SVM) classifier gene prediction. It uses radial basis function kernel...
Thousands of long noncoding RNAs (lncRNAs) have been found in vertebrate animals, a few which known biological roles. To better understand the genomics and features lncRNAs invertebrates, we used available RNA-seq, poly(A)-site, ribosome-mapping data to identify Caenorhabditis elegans . We 170 intervening ncRNAs (lincRNAs), had single- or multiexonic structures that did not overlap protein-coding transcripts, about sixty antisense (ancRNAs), were complementary transcripts. Compared genes,...
Argonaute is the primary mediator of metazoan miRNA targeting (MT). Among currently identified >1,500 human RNA-binding proteins (RBPs), there are only a handful RBPs known to enhance MT and several others reported suppress MT, leaving global impact on elusive. In this study, we have systematically analyzed transcriptome-wide binding sites for 150 evaluated quantitative effect individual efficacy. contrast previous studies, show that most significantly affect all those MT-regulating function...
Abstract Intratumor heterogeneity (ITH) is observed at different stages of tumor progression, metastasis and reouccurence, which can be important for clinical applications. We used RNA-sequencing data from samples, measured the level ITH in terms biological network states. To model complex relationships among genes, we a protein interaction to consider gene-gene dependency. was by using an entropy-based distance metric between two networks, nJSD, with Jensen-Shannon Divergence (JSD). With...
Wnt signaling through both canonical and noncanonical pathways plays a core role in development. Dysregulation of these often causes cancer development progression. Although the independently contribute to processes, regulatory molecule that commonly activates them has not yet been reported. Here, we describe long noncoding RNA (lncRNA), HERES, epigenetically regulates esophageal squamous cell carcinoma (ESCC). For this study, performed RNA-seq analysis on Korean ESCC patients validated...
The advent of high-throughput RNA sequencing (RNA-seq) has led to the discovery unprecedentedly immense transcriptomes encoded by eukaryotic genomes. However, transcriptome maps are still incomplete partly because they were mostly reconstructed based on RNA-seq reads that lack their orientations (known as unstranded reads) and certain boundary information. Methods expand usability data predetermining orientation precisely determining boundaries assembled transcripts could significantly...
Abstract Understanding the interactions between nanoparticles (NPs) and human immune cells is necessary for justifying their utilization in consumer products biomedical applications. However, conventional assays may be insufficient describing complexity heterogeneity of cell–NP interactions. Herein, mass cytometry single‐cell RNA‐sequencing (scRNA‐seq) are complementarily used to investigate heterogeneous silver (AgNPs) primary cells. Mass reveals biodistribution positively charged...