A5087589263- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Plant Molecular Biology Research
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Chinese history and philosophy
- Genetics, Aging, and Longevity in Model Organisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Origins and Evolution of Life
- Genomics and Chromatin Dynamics
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- RNA regulation and disease
- Seismic Waves and Analysis
- Plant Stress Responses and Tolerance
Whitehead Institute for Biomedical Research
2016-2025
Massachusetts Institute of Technology
2016-2025
Howard Hughes Medical Institute
2016-2025
IIT@MIT
2022
École des hautes études en sciences sociales
2009-2020
Musée de la Civilisation
2019
Cold Spring Harbor Laboratory
2019
Hong Kong Baptist University
2015
Mayo Clinic
2011
Memorial Sloan Kettering Cancer Center
2009
MicroRNAs (miRNAs) are small endogenous RNAs that pair to sites in mRNAs direct post-transcriptional repression. Many match the miRNA seed (nucleotides 2–7), particularly those 3′ untranslated regions (3′UTRs), preferentially conserved. Here, we overhauled our tool for finding preferential conservation of sequence motifs and applied it analysis human 3′UTRs, increasing by nearly threefold detected number conserved target sites. The new more efficiently incorporates genomes completely...
MicroRNA targets are often recognized through pairing between the miRNA seed region and complementary sites within target mRNAs, but not all of these canonical equally effective, both computational in vivo UV-crosslinking approaches suggest that many mRNAs targeted non-canonical interactions. Here, we show recently reported do mediate repression despite binding miRNA, which indicates vast majority functional canonical. Accordingly, developed an improved quantitative model targeting, using a...
MicroRNAs (miRNAs) are an abundant class of approximately 22-nucleotide regulatory RNAs found in plants and animals. Some miRNAs plants, Caenorhabditis elegans, Drosophila play important gene-regulatory roles during development by pairing to target mRNAs specify posttranscriptional repression these messages. We identify three that specifically expressed hematopoietic cells show their expression is dynamically regulated early hematopoiesis lineage commitment. One miRNAs, miR-181, was...
Two small temporal RNAs (stRNAs), lin-4 and let-7, control developmental timing in Caenorhabditis elegans. We find that these two regulatory are members of a large class 21- to 24-nucleotide noncoding RNAs, called microRNAs (miRNAs). report on 55 previously unknown miRNAs C. The have diverse expression patterns during development: let-7 paralog is temporally coexpressed with let-7; encoded single genomic cluster embryogenesis; still other expressed constitutively throughout development....
MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that processed by Dicer from precursors with a characteristic hairpin secondary structure. Guidelines presented for the identification and annotation of new miRNAs diverse organisms, particularly so can be reliably distinguished other RNAs such as interfering RNAs. We describe specific criteria experimental verification miRNAs, conventions naming miRNA genes. Finally, an online clearinghouse name assignments is...
MicroRNAs (miRNAs) are an extensive class of ∼22-nucleotide noncoding RNAs thought to regulate gene expression in metazoans. We find that miRNAs also present plants, indicating this RNA arose early eukaryotic evolution. In paper 16 Arabidopsis described, many which have differential patterns development. Eight absolutely conserved the rice genome. The plant miRNA loci potentially encode stem–loop precursors similar those processed by Dicer (a ribonuclease III) animals. Mutation homolog,...
MicroRNAs (miRNAs) are endogenous approximately 22-nucleotide RNAs, some of which known to play important regulatory roles in animals by targeting the messages protein-coding genes for translational repression. We find that miR-196, a miRNA encoded at three paralogous locations A, B, and C mammalian HOX clusters, has extensive, evolutionarily conserved complementarity HOXB8, HOXC8, HOXD8. RNA fragments diagnostic miR-196-directed cleavage HOXB8 were detected mouse embryos. Cell culture...