A5100744793- RNA modifications and cancer
- RNA Research and Splicing
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- Genomics and Phylogenetic Studies
- Identification and Quantification in Food
- Radioactive element chemistry and processing
- Supramolecular Chemistry and Complexes
- Autophagy in Disease and Therapy
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Molecular Sensors and Ion Detection
- RNA regulation and disease
- TGF-β signaling in diseases
- Histone Deacetylase Inhibitors Research
- Plant tissue culture and regeneration
- ATP Synthase and ATPases Research
- Pancreatic function and diabetes
- Telomeres, Telomerase, and Senescence
- Natural product bioactivities and synthesis
- Protein Kinase Regulation and GTPase Signaling
Catholic University of Korea
2015-2024
Korea Institute of Ocean Science and Technology
2014-2022
Dankook University
2020
Texas Tech University
2010-2016
Ewha Womans University
2015
Kyushu University
2013-2015
Ewha Womans University Medical Center
2015
Daegu Haany University
2014
Rural Development Administration
2012-2013
National Institute on Aging
2009-2012
RNA-binding proteins (RBPs) and microRNAs (miRNAs) are potent post-transcriptional regulators of gene expression. Here, we show that the RBP HuR reduced c-Myc expression by associating with 3′ untranslated region (UTR) next to a miRNA let-7-binding site. Lowering or let-7 levels relieved translational repression c-Myc. Unexpectedly, repressed through an interdependent mechanism, as required reduce inhibit Our findings suggest regulatory paradigm wherein inhibits recruiting let-7-loaded RISC...
Adipose tissue development is tightly regulated by altering gene expression.MicroRNAs are strong posttranscriptional regulators of mammalian differentiation.We hypothesized that microRNAs might influence human adipogenesis targeting specific adipogenic factors.We identified showed varying abundance during the differentiation preadipocytes into adipocytes.Among them, miR-130 strongly affected adipocyte differentiation, as overexpressing impaired and reducing enhanced adipogenesis.A key...
RNA-binding proteins (RBPs) regulate gene expression at many post-transcriptional levels, including mRNA stability and translation. The RBP nucleolin, with four RNA-recognition motifs, has been implicated in cell proliferation, carcinogenesis viral infection. However, the subset of nucleolin target mRNAs influence on their had not studied a transcriptome-wide level. Here, we globally identified transcripts, which encoded growth- cancer-related proteins, used them to find signature motif...
MicroRNAs (miRNAs) are short non-coding RNAs that regulate diverse biological processes by controlling the pattern of expressed proteins. In mammalian cells, miRNAs partially complement their target sequences leading to mRNA degradation and/or decreased translation. Here, we have analyzed transcriptome-wide changes in senescent relative early-passage WI-38 human diploid fibroblasts (HDFs). Among downregulated with senescence were members let-7 family, while upregulated included miR-1204,...
Neuronal development and plasticity are maintained by tightly regulated gene expression programs. Here, we report that the developmentally microRNA miR-375 affects dendrite formation maintenance. overexpression in mouse hippocampus potently reduced density. We identified predominantly neuronal RNA-binding protein HuD as a key effector of influence on Heterologous reporter analysis verified repressed through specific, evolutionarily conserved site 3′ untranslated region. lowered both mRNA...
An accurate tool enabling early diagnosis of hepatocellular carcinoma (HCC) is clinically important, given that detection HCC markedly improves survival. We aimed to investigate the molecular markers underlying progression can be detected in precancerous lesions. designed a gene selection strategy identify potential driver genes by integrative analysis transcriptome and clinicopathological data human multistage tissues, including lesions, low‐ high‐grade dysplastic nodules. The process was...
The RNA-binding protein (RBP) nucleolin promotes the expression of several proliferative proteins. Nucleolin levels are high in cancer cells, but mechanisms that control unknown. Here, we show abundance is controlled posttranscriptionally via factors associate with its 3' untranslated region (3'UTR). RBP HuR was found to interact (NCL) 3'UTR and specifically promoted translation without affecting mRNA levels. In human cervical carcinoma HeLa analysis a traceable NCL bearing MS2 RNA hairpins...
The RNA-binding protein nuclear factor 90 (NF90) has been implicated in the stabilization, transport and translational control of several target mRNAs. However, a systematic analysis NF90 mRNAs not performed. Here, we use ribonucleoprotein immunoprecipitation to identify large subset NF90-associated Comparison 3′-untranslated regions (UTRs) these led elucidation 25- 30-nucleotide, RNA signature motif rich adenines uracils. Insertion AU-rich ('NF90m') 3′UTR an EGFP heterologous reporter did...
Activation of cannabinoid 1 receptors prevents insulin from promoting β-cell survival in the pancreas.
The cellular abundance of topoisomerase II␣ (TOP2A) critically maintains DNA topology after replication and determines the efficacy TOP2 inhibitors in chemotherapy.Here, we report that RNA-binding protein HuR, commonly overexpressed cancers, binds to TOP2A 3-untranslated region (3UTR) increases translation.Reducing HuR levels triggered recruitment transcripts RNA-induced silencing complex (RISC) components cytoplasmic processing bodies.Using a novel MS2-tagged RNA precipitation method,...
Piwi-interacting RNAs (piRNAs) are 26–31 nt small noncoding that processed from their longer precursor transcripts by Piwi proteins. Localization of and piRNA has been reported mostly in nucleus cytoplasm higher eukaryotes germ-line cells, where it is believed known sequences located repeat regions nuclear genome cells. However, localization PIWI mammalian somatic cell mitochondria yet remains largely unknown. We identified 29 sequence alignments various the human mitochondrial genome....
Wnt signaling through both canonical and noncanonical pathways plays a core role in development. Dysregulation of these often causes cancer development progression. Although the independently contribute to processes, regulatory molecule that commonly activates them has not yet been reported. Here, we describe long noncoding RNA (lncRNA), HERES, epigenetically regulates esophageal squamous cell carcinoma (ESCC). For this study, performed RNA-seq analysis on Korean ESCC patients validated...
Although conventional topical approaches for treating psoriasis have been offered as an alternative, there are still unmet medical needs such low skin-penetrating efficacy and off-target adverse effects. A hyaluronic acid nanoparticle (HA-NP) formed by self-assembly of HA-hydrophobic moiety conjugates has broadly studied a nanocarrier long-term target-specific delivery drugs, owing to their excellent physicochemical biological characteristics. Here, we identify HA-NPs therapeutics using in...
Adipocytes are continuously stimulated by proinflammatory cytokines such as TNFα, which cause adipocyte dysfunction facilitating the inflammatory response. Although miR‐130 was reported to be an important regulator of adipogenesis targeting PPARγ mRNA, little is known about mechanisms regulating expression during Here, we examined levels in white adipose tissue (WAT) from high‐fat diet (HFD) mice and TNFα‐stimulated adipocytes. Primary transcripts were increased after TNFα stimulation,...