A5082376747- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genetic and Kidney Cyst Diseases
- Ferroptosis and cancer prognosis
- Peroxisome Proliferator-Activated Receptors
- Cancer-related gene regulation
- melanin and skin pigmentation
- Alzheimer's disease research and treatments
- ATP Synthase and ATPases Research
- Cancer-related molecular mechanisms research
- Cell death mechanisms and regulation
- RNA Research and Splicing
- Genetic factors in colorectal cancer
- Cancer, Hypoxia, and Metabolism
- Cancer therapeutics and mechanisms
- Glycosylation and Glycoproteins Research
- Endoplasmic Reticulum Stress and Disease
- Sulfur Compounds in Biology
- DNA Repair Mechanisms
- Calcium signaling and nucleotide metabolism
- Ubiquitin and proteasome pathways
- Sirtuins and Resveratrol in Medicine
- Colorectal Cancer Treatments and Studies
Kyungpook National University
2018-2025
Suwon Research Institute
2025
Institute for Basic Science
2024
Kyung Hee University
2011-2021
Asan Medical Center
2008-2019
Kyung Hee University East-West Neo Medical Center
2014-2017
University of Ulsan
2008-2010
Sanford Burnham Prebys Medical Discovery Institute
2007-2010
Ulsan College
2008-2010
Gwangju Institute of Science and Technology
2004
Mitochondria continuously undergo two opposing processes, fission and fusion. The disruption of this dynamic equilibrium may herald cell injury or death contribute to developmental neurodegenerative disorders. Nitric oxide functions as a signaling molecule, but in excess it mediates neuronal injury, part via mitochondrial fragmentation. However, the underlying mechanism for nitric oxide–induced pathological remains unclear. We found that produced response β-amyloid protein, thought be key...
Excessive mitochondrial fission is a prominent early event and contributes to dysfunction, synaptic failure, neuronal cell death in the progression of Alzheimer's disease (AD). However, it remains be determined whether inhibition excessive beneficial mammal models AD. To determine dynamin-related protein 1 (Drp1), key regulator fragmentation, can disease-modifying therapeutic target for AD, we examined effects Drp1 inhibitor on dysfunctions induced by oligomeric amyloid-β (Aβ) neurons...
Peroxiredoxins (Prx), a family of peroxidases that reduce intracellular peroxides with the thioredoxin system as electron donor, are highly expressed in various cellular compartments. Among antioxidant Prx enzymes, Prx2 is most abundant mammalian neurons, making it prime candidate to defend against oxidative stress. Here we report S-nitrosylated (forming SNO-Prx2) by reaction nitric oxide at two critical cysteine residues (C51 and C172), preventing its peroxides. We observed increased...
Colorectal cancer (CRC) patients frequently experience disease recurrence and distant metastasis. This study aimed to identify prognostic indicators, including individual responses chemotherapy, in CRC patients. RNA‐seq data was generated using 54 samples (normal colon, primary CRC, liver metastases) from 18 genes associated with aggressiveness were identified. A risk score based on these developed validated four independent patient cohorts ( n = 1063). Diverse statistical methods applied...
We screened a chemical library in MCF-7 cells stably expressing green fluorescent protein (GFP)-conjugated microtubule-associated 1 light chain 3 (LC3) (GFP-LC3-MCF-7) using cell-based assay, and identified BIX-01294 (BIX), selective inhibitor of euchromatic histone-lysine N-methyltransferase 2 (EHMT2), as strong autophagy inducer. BIX enhanced formation GFP-LC3 puncta, LC3-II, free GFP, signifying autophagic activation. Inhibition these phenomena with chloroquine increasement punctate...
Lysophagy eliminates damaged lysosomes and is crucial to cellular homeostasis; however, its underlying mechanisms are not entirely understood. We screen a ubiquitination-related compound library determine that the substrate recognition component of SCF-type E3 ubiquitin ligase complex, SCFFBXO3(FBXO3), which critical lysophagy regulator. Inhibition FBXO3 reduces lysophagic flux in response L-leucyl-L-leucine methyl ester (LLOMe). Furthermore, interacts with TMEM192, leading ubiquitination...
Background Autophagy has paradoxical and complex functions in cancer development, autophagy-related genes (ATG) are key regulators autophagy. Until now, more than 30 different ATG proteins have been identified yeast, their mammalian counterparts also reported. Although the roles of a few characterized, role ATG10 is almost completely unknown. Methodology/Principal Findings To investigate clinicopathological colorectal cancer, we analyzed expression tissues cell lines. Protein analysis showed...
A primary cilium is an antenna-like structure on the cell surface that plays a crucial role in sensory perception and signal transduction. Mitochondria, 'powerhouse' of cell, control survival, death. The cellular ability to remove dysfunctional mitochondria through mitophagy important for survival. We show here mitochondrial stress, caused by respiratory complex inhibitors excessive fission, robustly stimulates ciliogenesis different types cells including neuronal cells. Mitochondrial...
Mitochondrial quality control maintains mitochondrial function by regulating dynamics and mitophagy. Despite the identification of factors, little is known about crucial regulators coordinating both fission Through a cell-based functional screening assay, FK506 binding protein 8 (FKBP8) was identified to target microtubule-associated 1 light chain 3 (LC3) mitochondria change morphology. Microscopy analysis revealed that formation tubular enlarged observed in FKBP8 knockdown HeLa cells cortex...
Peroxisomes are rapidly degraded during amino acid and oxygen deprivation by a type of selective autophagy called pexophagy. However, how damaged peroxisomes detected removed from the cell is poorly understood. Recent studies suggest that peroxisomal matrix protein import machinery may serve double duty as quality control machinery, where they directly involved in activating Here, we explored whether any factors required to prevent pexophagy, such their loss designates for degradation. Using...
Mitochondrial dynamics not only involves mitochondrial morphology but also biogenesis, distribution, and cell death. To identify specific regulators to mitochondria dynamics, we screened a chemical library identified niclosamide as potent inducer of fission. Niclosamide promoted fragmentation this was blocked by down-regulation Drp1. treatment resulted in the disruption membrane potential reduction ATP levels. Moreover, led apoptotic death caspase-3 activation. Interestingly, increased...
Objectives:To evaluate the association of microsatellite instability (MSI) with clinicopathologic features and oncologic outcomes in patients poorly differentiated colorectal cancer (PD).