A5084014218- Nanoparticle-Based Drug Delivery
- Cancer Research and Treatments
- Click Chemistry and Applications
- Nanoplatforms for cancer theranostics
- Retinoids in leukemia and cellular processes
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Cancer-related Molecular Pathways
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- Photochromic and Fluorescence Chemistry
- Epigenetics and DNA Methylation
- Hepatitis B Virus Studies
- Lung Cancer Diagnosis and Treatment
- Bioactive Compounds and Antitumor Agents
- Lung Cancer Treatments and Mutations
- Histone Deacetylase Inhibitors Research
- Immunotherapy and Immune Responses
- RNA regulation and disease
- S100 Proteins and Annexins
- Endoplasmic Reticulum Stress and Disease
- Lung Cancer Research Studies
- Pancreatic and Hepatic Oncology Research
Asan Medical Center
2015-2024
University of Ulsan
2015-2024
Ulsan College
2015-2024
Park University
2023
Korea University
2022
Seoul Medical Center
2014-2018
Duksung Women's University
2015
Chungnam National University
2012
Inha University
2008
Pusan National University
2007
Abstract Lung cancer shows substantial genetic and phenotypic heterogeneity across individuals, driving a need for personalised medicine. Here, we report lung organoids normal bronchial established from patient tissues comprising five histological subtypes of non-neoplastic mucosa as in vitro models representing individual patient. The recapitulate the tissue architecture primary tumours maintain genomic alterations original during long-term expansion vitro. cellular components mucosa....
We screened a chemical library in MCF-7 cells stably expressing green fluorescent protein (GFP)-conjugated microtubule-associated 1 light chain 3 (LC3) (GFP-LC3-MCF-7) using cell-based assay, and identified BIX-01294 (BIX), selective inhibitor of euchromatic histone-lysine N-methyltransferase 2 (EHMT2), as strong autophagy inducer. BIX enhanced formation GFP-LC3 puncta, LC3-II, free GFP, signifying autophagic activation. Inhibition these phenomena with chloroquine increasement punctate...
Abstract Gambogic acid (GA), a xanthonoid extracted from the resin of tree, Garcinia hanburyi , was recently shown to exert anticancer activity in multiple studies, but underlying action mechanism remains unclear. Here, we show that GA induces cancer cell death accompanied by vacuolation vitro and vivo. This GA-induced various cells derived dilation endoplasmic reticulum (ER) mitochondria, blocked cycloheximide. These findings suggest kills inducing paraptosis, vacuolization-associated...
Valosin-containing protein (VCP)/p97, an AAA+ ATPase critical for maintaining proteostasis, emerges as a promising target cancer therapy. This study reveals that targeting VCP selectively eliminates breast cells while sparing non-transformed by inducing paraptosis, non-apoptotic cell death mechanism characterized endoplasmic reticulum and mitochondria dilation. Intriguingly, oncogenic HRas sensitizes to inhibition-mediated paraptosis. The susceptibility of inhibition is attributed the...
Raloxifene is a selective estrogen receptor modulator (SERM) that binds to the (ER), and exhibits potent anti-tumor autophagy-inducing effects in breast cancer cells. However, mechanism of raloxifene-induced cell death autophagy not well-established. So, we analyzed underlying induced by raloxifene MCF-7 Treatment with significantly accumulated GFP-LC3 puncta increased level autophagic marker proteins, such as LC3-II, BECN1, ATG12-ATG5 conjugates, indicating activated autophagy. also flux...
Limited data describe the prognosis after stereotactic ablative radiotherapy for lung metastases arising from colorectal cancer. Thus, we evaluated treatment outcomes of those patients. The study involved patients received one to three cancer at a single institution. A total dose 40–60 Gy (median, 48 Gy) in or four fractions was prescribed. 79 metastatic lesions 50 who underwent curative resection their primary salvage recurrent site were included. one- and three-year local control rates...
Radiotherapy (RT) has been used for decades as one of the main treatment modalities cancer patients. The therapeutic effect RT primarily ascribed to DNA damage leading tumor cell death. Besides direct tumoricidal effect, affects antitumor responses through immune-mediated mechanism, which provides a rationale combining and immunotherapy treatment. Thus far, combined with RT, numerous studies have focused on immune checkpoint inhibitors shown promising results. However, resistance is still...
Abstract Metastatic colorectal cancer (CRC) remains a substantial problem for mortality and requires screening early detection efforts to increase survival. Epithelial-mesenchymal transition (EMT) circulation of tumor cells in the blood play important roles metastasis. To identify novel target metastasis CRC, we conducted gene microarray analysis using extracted RNA from preclinical models. We found that NCK-associated protein 1 (NCKAP1) was significantly increased patient-derived xenograft...
Claspin is essential for the ATR-dependent activation of Chk1 in Xenopus egg extracts containing incompletely replicated or UV-damaged DNA. The activated form contains two repeated phosphopeptide motifs that mediate its binding to Chk1. We show these bind by means N-terminal kinase domain. site on involves a positively charged cluster amino acids lysine 54, arginine 129, threonine 153, and 162. Mutagenesis residues strongly compromises ability interact with Claspin. These lie within regions...
// Sang Eun Park 1, 7 , Hye Jin Yi 1 Nayoung Suh 9 Yun-Yong 2, 5 Jae-Young Koh 3, 4, 6 Seong-Yun Jeong Dong-Hyung Cho 8 Choung-Soo Kim Jung Hwang Institute for Innovative Cancer Research, Asan Medical Center, Seoul, Korea 2 Life Sciences, 3 Department of Neurology, 4 Urology, Convergence Medicine, University Ulsan, College Neural Injury Research Lab, Graduate School East-West Science, Kyung Hee University, Yongin, Medicine Engineering, Soon Chun Hyang Asan, Correspondence to: Hwang, e-mail:...
To evaluate the role of radiotherapy (RT) as an adjuvant or definitive treatment in primary tracheal adenoid cystic carcinoma (ACC) for local tumor control and survival. A retrospective chart review was performed 22 patients treated with RT ACC at a single center between November 1994 December 2008. Thirteen 9 received RT, respectively. Microscopic residual disease after surgery pathologically reported 11 patients. The median dose 59.4 Gy 74.4 RT. overall response rate 77.8%. Six group...
Human epidermal growth factor receptor 2 (HER2) is overexpressed in breast and gastric cancers this causes poor clinical outcomes. Although both T-DM1 Enhertu are approved as an HER2-targeting antibody-drug conjugate (ADC), the effects of these drugs still not satisfactory to eradicate diverse tumors expressing HER2. To address shortfall HER2-targeted therapeutics, elaborate cleavable linker created a novel ADC composed with trastuzumab monomethyl auristatin F, which being investigated phase...
The checkpoint mediator protein Claspin is indispensable for the ATR-dependent phosphorylation of Chk1 in response to stalled DNA replication forks Xenopus egg extracts. We show that also participates detection chromosomal double-stranded breaks (DSBs) this system. Significantly, removal from extracts only partially abrogates activation chromatin with DSBs, whereas depletion both and BRCA1 completely abolishes activation. function DSB-triggered pathway depends on T817 S819 by ATR....
Abstract: Nanoparticles are useful delivery vehicles for promising drug candidates that face obstacles clinical applicability. Sirolimus, an inhibitor of mammalian target rapamycin has gained attention targeted anticancer therapy, but its application been limited by poor solubility. This study was designed to enhance the feasibility sirolimus human cancer treatment. Polymeric nanoparticle (PNP)–sirolimus developed as injectable formulation and characterized transmission electron microscopy...
We investigated the use of hyperthermia to improve anti-cancer efficacy doxorubicin (DOX)-loaded mesoporous silica nanocontainer Si-SS-CD-PEG. The hypothesis was that heat stimulates glutathione-mediated degradation cyclodextrin gatekeeper, thereby causing release DOX from carrier and DOX-induced cell death.The DOX-loaded Si-SS-CD-PEG suspended in PBS containing glutathione (GSH) studied by assessing changes fluorescence intensity. effect heating at 42°C on intracellular carriers determined...
Docetaxel (DTX) is an effective commercial anticancer agent for chemotherapy in non-small cell lung cancer (NSCLC), breast cancer, gastric and prostate but its adverse effects including edema, neurotoxicity, hair loss limit application. To improve the chemotherapeutic efficacy of DTX reduce effects, combination therapy one alternative methods. So chrysin, which has various biological activities was considered. In vitro, chrysin investigated A549 cells. Increased cytotoxicity, suppressed...