A5037547895- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Chinese history and philosophy
- Lipid metabolism and biosynthesis
- RNA Research and Splicing
- Body Contouring and Surgery
- Lymphatic System and Diseases
- Hippo pathway signaling and YAP/TAZ
- Sympathectomy and Hyperhidrosis Treatments
- Microtubule and mitosis dynamics
- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Lung Cancer Research Studies
- Protein Kinase Regulation and GTPase Signaling
- Medical Imaging and Pathology Studies
- Cell Image Analysis Techniques
- Gene expression and cancer classification
- Renal and related cancers
- Heat shock proteins research
- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
- AI in cancer detection
Asan Medical Center
2014-2024
Roswell Park Comprehensive Cancer Center
2022-2024
Korea University
2024
Bio-Medical Science (South Korea)
2024
University of Ulsan
2019-2022
Ulsan College
2019-2022
Southerners on New Ground
2019
Korea Advanced Institute of Science and Technology
2016
Abstract Lung cancer shows substantial genetic and phenotypic heterogeneity across individuals, driving a need for personalised medicine. Here, we report lung organoids normal bronchial established from patient tissues comprising five histological subtypes of non-neoplastic mucosa as in vitro models representing individual patient. The recapitulate the tissue architecture primary tumours maintain genomic alterations original during long-term expansion vitro. cellular components mucosa....
Microfluidic devices as translational research tools offers opportunities to aid in reliable medical decision making and develop customized patient care through predictive modeling of drug sensitivity patient-derived cancer organoids.
Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease with limited effective treatment options, potentiating the importance of uncovering novel drug targets. Here, we target cleavage and polyadenylation specificity factor 3 (CPSF3), 3′ endonuclease that catalyzes mRNA during histone processing. We find CPSF3 highly expressed in PDAC associated poor prognosis. knockdown blocks cell proliferation colony formation vitro tumor growth vivo. Chemical inhibition by small molecule JTE-607 also...
The intrinsic immuno-ge7nomic characteristics of colorectal cancer cells that affect tumor biology and shape the immune microenvironment (TIM) are unclear.We developed a patient-derived organoid (CCO) model performed pairwise analysis 87 CCOs their matched primary tumors. TIM type was classified as immuno-active, immuno-exhausted, or immuno-desert.The gene expression profiles, signaling pathways, major oncogenic mutations, histology recapitulated those tumors, but not Two distinct molecular...
Aberrant Wnt/β-catenin signalling is implicated in the progression of several human cancers, including non-small cell lung cancer (NSCLC). However, mutations pathway components are uncommon NSCLC, and their epigenetic control remains unclear. Here, we show that KIF3A, a member kinesin-2 family, plays role suppressing NSCLC cells. KIF3A knockdown increases both β-catenin levels transcriptional activity with concomitant promotion malignant potential, such as increased proliferation migration...
Abstract The human cutaneous lymphatic system strictly controls functions by coordinating with skin cells. plays important roles in removing cell waste, residual proteins, various antigens, and immune cells from tissues to maintain homeostasis activate the through drainage of interstitial fluid 1,2 . protects our body external stimuli such as pathogens 3,4 Herein, develop an vitro model, we present two 3D microfluidic platforms: a lymphangiogenesis model precollecting vessel-like structure...
Organoids and 3D imaging techniques are crucial for studying human tissue structure function, but traditional reconstruction methods expensive time consuming, relying on complete z stack confocal microscopy data. This paper introduces VONet, a deep learning-based system organoid rendering that uses fully convolutional neural network to reconstruct entire structures from minimal number of images. VONet was trained library over 39,000 virtual organoids (VOs) with diverse structural features...
Abstract Purpose Although epidermal growth factor receptor (EGFR)-activating mutations in non-small cell lung cancer (NSCLC) usually show sensitivity to first-generation EGFR-tyrosine kinase inhibitors (TKIs), most patients relapse because of drug resistance. Heat shock protein 27 (HSP27) has been reported be involved the resistance EGFR-TKIs, although underlying mechanism is unclear. Here, we explore mechanisms HSP27-mediated EGFR TKI and propose novel therapeutic strategies. Methods To...
Abstract As research into the complexities of cancer biology deepens, integration multi-omics analyses has emerged as a powerful approach to unravel complex molecular basis cancers. However, challenges related sample availability, including size, collection procedures, and storage duration, hinder broad application this methodology. Despite these limitations, there is growing interest in exploring potential archived samples expand scope research. Our study aims investigate impact duration on...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease with limited effective treatment options. This potentiates the importance of uncovering novel drug targets. We have discovered global dysregulation gene regulatory process alternative polyadenylation (APA) in PDAC. APA pre-mRNA processing mechanism that generates mRNAs distinct 3’ ends, impacting expression and protein function. revealed PDAC drives oncogenic signatures predicts poor patient outcome. As directs widespread...
Abstract Human cancers are composed of genetically and phenotypically heterogeneous subpopulations organized into a hierarchy tumor initiating stem cells various differentiated cell. Cancer organoids seem to be more suitable in vitro models for targeting heterogeneity than cell lines. We established cancer organoid lines from lung patient tissues, recapitulating histology human three dimensional culture conditions. Thirteen subtypes including adenocarcinoma, squamous carcinoma small were...
The data presented in this article is related to a rapid communication entitled "β-arrestin 2 suppresses the activation of YAP by promoting LATS kinase activity". This describes correlation β-arrestin and phosphorylation patient-derived organoid models. Here, we analyzed 45 colon cancer organoids (CCOs) selected research investigate role phosphorylation. Hematoxylin eosin (H&E) staining immunohistochemistry showed that CCOs maintained tissue architecture histological features their original...
Cancer model systems that maintain the genetic and phenotypic characteristics of human cancers are crucial for study precision cancer medicine. In this respect, patient-derived organoids have been developed as preclinical models various cancers, with significant advantages over previous including xenografts cell lines. We recently reported a culture system lung (LCOs) retain patients’ tumors. Here, we describe detailed protocol establishing LCOs from surgically resected tumor tissues...
Abstract Lung cancer is a leading cause of death in worldwide. 85% lung cancers are classified as non-small cell (NSCLC), and adenocarcinoma (ADC) type the most prevalent NSCLC. Approximately 50% (NSCLC) patients have mutations some oncogenes. The K-ras activation due to mutant notorious many kinds types, including pancreatic adenocarcinoma, generally accepted driving mutation for malignancy adenocarcinoma. Another predominantly alternated gene EGFR that would hit similar signaling with...
Abstract Aberrant Wnt/β-catenin signaling is implicated in the progression of several human cancers, including non-small-cell lung cancer (NSCLC). However, mutations pathway com-ponents are uncommon NSCLC, and epigenetic mechanisms controlling path-way remain unclear. Here, we show that KIF3A, a member kinesin-2 motor family, plays key role suppressing NSCLC cells. Knockdown KIF3A increases both β-catenin levels transcriptional activity, with concomitant promotion malignant phenotypes, such...
Abstract The human cutaneous lymphatic system strictly controls the functions as coordinating with skin cells. plays important roles in removing cell waste, residual proteins, various antigens, and immune cells from tissues order to maintain homeostasis activate system, through drainage of interstitial fluid 1,2 . protects our body external stimulates such pathogens 3,4 Herein, develop vitro model, we present two 3D microfluidic platforms: a lymphangiogenesis model pre-collecting vessel-like...
Since the 1894 plague epidemic, Hong Kong’s colonial medicine had improved, which in part accompanied by economic growth given Kong being an import node of British Empire’s Asian trade network. Yet, has emphasized ‘prevention infectious diseases’ and ‘quarantine’ strategy ‘medical service’ was left to medical market. Charitable organizations, especially that Chinese elites, devoted themselves meeting shortage public services; such approach aligned with British’s intention minimizes...
Abstract Treatment regimens of pancreatic ductal adenocarcinoma (PDAC) are limited and minimally effective which potentiates the importance discovering novel potential drug targets. We discovered widespread dysregulation alternative polyadenylation (APA) in PDAC patients. APA is a pre-mRNA processing mechanism that generates mRNAs isoforms with distinct 3’ untranslated regions (3’UTRs). These contribute to expression function proteins. Our recent study revealed alterations drive...