A5068700705- Genomics and Chromatin Dynamics
- Protein Kinase Regulation and GTPase Signaling
- PI3K/AKT/mTOR signaling in cancer
- Skin and Cellular Biology Research
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- HER2/EGFR in Cancer Research
- Cell death mechanisms and regulation
- Cancer Cells and Metastasis
- Nuclear Structure and Function
- Metabolism, Diabetes, and Cancer
- TGF-β signaling in diseases
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Connective Tissue Growth Factor Research
- Animal Genetics and Reproduction
- Genomics, phytochemicals, and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- Estrogen and related hormone effects
- Fungal and yeast genetics research
- Advanced Biosensing Techniques and Applications
- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- Cancer Mechanisms and Therapy
- Phagocytosis and Immune Regulation
Hanyang University
2016-2025
Mayo Clinic in Arizona
2025
Pusan National University
2022-2023
RELX Group (Netherlands)
2023
Korea University
2022
Life University
1997-2017
Anyang University
2014
Konkuk University
2013
Korea Research Institute of Bioscience and Biotechnology
2013
In-Q-Tel
2009
We have examined the interaction of transforming growth factor (TGF)beta receptors with phosphatidylinositol 3-(PI3) kinase in epithelial cells. In COS7 cells, treatment TGFbeta increased PI3 activity as measured by ability p85-associated immune complexes to phosphorylate inositides vitro. Both type I and II (TbetaR) associated p85, but association TbetaRII appeared be constitutive. The TbetaRI p85 was induced TGFbeta. receptor not direct (35)S-labeled rabbit reticulocyte did couple fusion...
Elevated glucose levels in cancer cells can be attributed to increased of transporter (GLUT) proteins. Glut1 expression is human malignant cells. To investigate alternative roles breast cancer, we silenced triple-negative breast-cancer cell lines using a short hairpin RNA (shRNA) system. silencing was verified by Western blotting and qRT-PCR. Knockdown resulted decreased proliferation, uptake, migration, invasion through modulation the EGFR/ MAPK signaling pathway integrin β1/Src/FAK...
Abstract Background Solid tumors promote tumor malignancy through interaction with the microenvironment, resulting in difficulties treatment. Therefore, it is necessary to understand communication between cells and surrounding microenvironment. Our previous study revealed cancer mechanism of Bcl-w overexpressed solid tumors, but no was conducted on its relationship immune In this study, we sought discover key factors exosomes secreted from overexpressing analyze microenvironment identify...
The Forkhead family of transcription factors participates in the induction death-related genes. In NMuMG and 4T1 mammary epithelial cells, transforming growth factor β (TGFβ) induced phosphorylation cytoplasmic retention FKHRL1, while reducing FHKRL1-dependent transcriptional activity. TGFβ-induced FKHRL1 nuclear exclusion were inhibited by LY294002, an inhibitor phosphatidylinositol-3 kinase. A triple mutant which all three Akt sites have been mutated (TM-FKHRL1), did not translocate to...
We generated a p27(Kip1) mutant (p27deltaNLS) that localized exclusively in cell cytosol. Expression of p27deltaNLS MCF7 breast cancer cells down-regulated RhoA and increased motility, survival, Akt levels without an effect on cycle distribution. RNA interference p27 U87 glioma cells, which express predominantly the cytoplasm, inhibited motility survival. Conversely, knockdown COS7 with >95% nuclear expression, accelerated proliferation but had no or been eliminated by exhibited lower...
Abstract In HER2 (ErbB2)-overexpressing cells, transforming growth factor β (TGF-β), via activation of phosphoinositide-3 kinase (PI3K), recruits actin and actinin to HER2, which then colocalizes with Vav2, activated Rac1, Pak1 at cell protrusions. This results in prolonged Rac1 activation, enhanced motility invasiveness, Bad phosphorylation, uncoupling Bad/Bcl-2, survival. The recruitment the HER2/Vav2/Rac1/Pak1/actin/actinin complex lamellipodia was abrogated by siRNAs, dominant-negative...
Abstract Introduction Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein with putative functions in cell proliferation, angiogenesis and differentiation. Expression of ECM1 several types carcinoma suggests that it may promote tumor development. In this study, we investigated the role oncogenic signaling breast cancer, potential mechanisms for its effects. Methods order to find out functional ECM1, used recombinant human viral transduction systems which stably regulated...
Abstract Purpose: Keratin19 (KRT19) is the smallest known type I intermediate filament and used as a marker for reverse transcriptase PCR–mediated detection of disseminated tumors. In this study, we investigated functional analysis KRT19 in human breast cancer. Experimental Design: Using short hairpin RNA system, silenced cancer cells. silencing was verified by Western blot immunocytochemistry. We further examined effect on cells cell proliferation, migration, invasion, colony formation...
Cluster of differentiation 44 (CD44) is a transmembrane glycoprotein that has been identified as cancer stem cell marker in various cells. Although many studies have focused on CD44 marker, its effect metabolism remains unclear. To investigate the role metabolism, we established knock-down cells via retroviral delivery shRNA against human breast Silencing decreased glycolytic phenotype cells, affecting glucose uptake, ATP production, and lactate production. We also found ablation...
Abstract Understanding the interactions between nanoparticles (NPs) and human immune cells is necessary for justifying their utilization in consumer products biomedical applications. However, conventional assays may be insufficient describing complexity heterogeneity of cell–NP interactions. Herein, mass cytometry single‐cell RNA‐sequencing (scRNA‐seq) are complementarily used to investigate heterogeneous silver (AgNPs) primary cells. Mass reveals biodistribution positively charged...
Abstract Triple-negative breast cancer (TNBC) is the most aggressive subtype of cancer. TNBC patients typically exhibit unfavorable outcomes due to its rapid growth and metastatic potential. Here, we found overexpression CCN3 in patients. We identified that knockdown diminished stem cell formation, metastasis, tumor vitro vivo. Mechanistically, ablation reduced activity EGFR/MAPK pathway. Transcriptome profiling revealed induces glycoprotein nonmetastatic melanoma protein B (GPNMB)...
Recent developments in tau positron emission tomography (PET) radiotracers have enhanced the visualization of aggregates Alzheimer's disease (AD). The maturity level neurofibrillary tangles can affect its recognition by biomarkers. Early detection regarding tangle pathology is interest early diagnosis and comparison this aspect important. This study focused on head to pathologic [18F]MK-6240 [18F]Flortaucipir postmortem binding as seen high resolution autoradiography compared CP-13 (early...
Nidogen-1 (NID1) is a secreted glycoprotein widely distributed in basement membranes. NID1 interacts with extracellular matrix proteins such as collagen and laminin has been implicated the progression of various cancers. However, study on role breast cancer scarce inconsistent. In this work, we found that expression significantly lower tissue than normal tissue. addition, correlated negatively poor prognosis for patients. Based those findings, speculated might act suppressor cancer. To...