A5091782076- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- RNA modifications and cancer
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Insect-Plant Interactions and Control
- Blood groups and transfusion
- Study of Mite Species
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Genetics, Aging, and Longevity in Model Organisms
- Blood disorders and treatments
- Neurological diseases and metabolism
- Blood properties and coagulation
- Reproductive Biology and Fertility
- Chromosomal and Genetic Variations
- Phytase and its Applications
- Plant and Fungal Interactions Research
PEN American Center
2021
CrystalGenomics (South Korea)
2018
Insilicogen (South Korea)
2014
National Institute of Diabetes and Digestive and Kidney Diseases
2009-2013
National Institutes of Health
2008-2013
Institute of Molecular Medicine
2009
Korea Research Institute of Bioscience and Biotechnology
2007
Pohang University of Science and Technology
1998
Abstract Non-self epitopes, whether originated from foreign substances or somatic mutations, trigger immune responses when presented by major histocompatibility complex (MHC) molecules and recognized T cells. Identification of immunogenically active neoepitopes has significant implications in cancer virus medicine. However, current methods are mostly limited to predicting physical binding mutant peptides MHCs. We previously developed a deep-learning based model, DeepNeo, identify immunogenic...
Abstract Background MicroRNAs are ~22nt-long small non-coding RNAs that negatively regulate protein expression through mRNA degradation or translational repression in eukaryotic cells. Based upon their importance regulating development and terminal differentiation model systems, erythrocyte microRNA profiles were examined at birth adults to determine if changes abundance coincide with the developmental phenomenon of hemoglobin switching. Methods Expression profiling was performed using total...
Genes, RNAs, and proteins play important roles during germline development. However, the functions of non-coding RNAs (ncRNAs) on development remain unclear in avian species. Recent high-throughput techniques have identified several classes ncRNAs, including micro (miRNAs), small-interfering (siRNAs), PIWI-interacting (piRNAs). These ncRNAs are functionally genome, however, identification annotation a genome is challenging. The aim this study was to identify different types small...
Leptotrombidium pallidum and scutellare are the major vector mites for Orientia tsutsugamushi, causative agent of scrub typhus. Before these organisms can be subjected to whole-genome sequencing, it is necessary estimate their genome sizes obtain basic information establishing strategies that should used sequencing assembly. The L. were estimated by a method based on quantitative real-time PCR. In addition, k-mer analysis sequences obtained through Illumina was conducted verify mutual...
Biomarkers for predicting response to the immunotherapy and chemotherapy combination in breast cancer patients are not established. In this study, we report exploratory genomic transcriptomic analyses of pretreatment tumor tissues from enrolled phase II clinical trial a eribulin nivolumab HER-2-negative metastatic (MBC) (KORNELIA trial, NCT04061863).
Genomic hypomethylation has recently been identified as a determinant of therapeutic responses to immune checkpoint blockade (ICB). However, it remains unclear whether this approach can be applied cell-free DNA (cfDNA) and address the issue low tumor purity encountered in tissue-based methylation profiling. In study, we developed an assay named iMethyl, designed estimate genomic status from cfDNA. This was achieved through deep targeted sequencing young LINE-1 elements with > 400,000 reads...
Based upon the lack of clinical samples available for research in many laboratories worldwide, a significant gap exists between basic and studies beta-thalassemia major. To bridge this gap, we developed an artificially engineered model human beta thalassemia by knocking down beta-globin gene protein expression cultured CD34+ cells obtained from healthy adults. Lentiviral-mediated transduction shRNA (beta-KD) caused imbalanced globin chain production. Beta-globin mRNA was reduced 90% compared...
We have sequenced and characterized the complete mitochondrial DNA of an economically ecologically important Pacific abalone, Haliotis discus hannai (Haliotidae, Gastropoda). The mitogenome abalone is 16,886 nt total length with a 39.6% G + C composition. Thirty-seven genes were identified including 13 protein-coding, 2 rRNA 22 tRNA genes. compared to putative relative species, H. rubra.
The identification of the initial signaling events induced by progesterone in Xenopus oocyte maturation was approached expressing serotonin receptors and using pharmacological agents. Suppression phospholipase C (PLC) activity oocytes U-73122 or stimulation adenylyl cyclase (ACase) forskolin inhibited progesterone-induced reinitiation meiotic cell division. Microinjection cAMP-dependent protein kinase (PKA) pseudosubstrate, PKI, into oocytes--or pretreatment with PKA inhibitor, H-89--did not...
Tissue-specific gene expression generates fundamental differences in the function of each tissue and affects characteristics tumors that are created as a result. However, it is unclear how much specificity conserved during grafting primary tumor into an immune-compromised mouse model. Here, we performed comparative RNA-seq analysis four different primary-patient derived xenograft (PDX) tumors. The revealed RNA biotype distribution primary−PDX pairs, by previous works. Interestingly, detected...
In the finely regulated process of mammalian erythropoiesis, path labile iron pool into mitochondria for heme production is not well understood. Existing models erythropoiesis do include a central role ubiquitous storage protein ferritin; one model proposes that incoming endosomal Fe3+ bound to transferrin enters cytoplasm through an ion transporter after reduction Fe2+ and taken up mitoferrin-1 transporter. Here, we apply dual three-dimensional imaging spectroscopic technique, based on...
Abstract Much is known about the finely regulated process of mammalian erythropoiesis that occurs in bone marrow, whereby erythropoietic stem cells undergo terminal differentiation accompanied by enormous morphological changes to generate highly functional specialized red blood cells. However, a crucial step erythropoiesis, labile iron pool and its transport mitochondria for heme production, not well understood 1 . We apply dual 3D imaging spectroscopic technique, based on scanned electron...