A5079035482- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Dermatological diseases and infestations
- Cholinesterase and Neurodegenerative Diseases
- Plant Virus Research Studies
- Entomopathogenic Microorganisms in Pest Control
- Study of Mite Species
- Insects and Parasite Interactions
- Forest Insect Ecology and Management
- Bird parasitology and diseases
- Genetic diversity and population structure
- Nematode management and characterization studies
- Research on scale insects
- Helminth infection and control
- Hemiptera Insect Studies
- Fungal Plant Pathogen Control
- Plant Disease Management Techniques
- Environmental Toxicology and Ecotoxicology
- Plant Pathogens and Fungal Diseases
- Vector-borne infectious diseases
- Medicinal Plants and Neuroprotection
- Insect symbiosis and bacterial influences
- Bacterial Genetics and Biotechnology
Seoul National University
2012-2024
Rural Development Administration
2020-2021
National Institute of Agricultural Science and Technology
2018
Hallym University
2018
Georgia State University
1994-1997
This study establishes deltamethrin resistance in a common bed bug, Cimex lectularius L., population collected from New York City (NY-BB). The NY-BB was 264-fold more resistant to 1% contact bioassay compared with an insecticide-susceptible Florida (FL-BB). General esterase, glutathione S-transferase, and 7-ethoxycoumarin O-deethylase activities of were not statistically different those FL-BB. cDNA fragments that encoded the open reading frame voltage-sensitive sodium channel alpha-subunit...
The molecular mechanisms and genetics of abamectin resistance mediated by target site insensitivity in the two-spotted spider mite, Tetranychus urticae, were investigated comparing two isogenic abamectin-susceptible (AbaS) abamectin-resistant (AbaR) strains. Cloning sequencing full-length cDNA fragments gamma-amino butyric acid (GABA)-gated chloride channel genes revealed no polymorphisms between However, sequence comparison fragment a T. urticae glutamate-gated gene (TuGluCl) identified...
Monocrotophos-resistant two-spotted spider mites (TSSMs), Tetranychus urticae, are known to possess three mutations on the acetylcholinesterase (AChE) gene (Tuace) that involved in target site insensitivity. Cross-strain comparison of strains (highly resistant AD, moderately PyriF and susceptible UD strains) revealed have relatively more Tuace copies than strain levels transcript were directly proportional copy numbers. AChEs from AD had similar V(max) values those AChE but increased K(m)...
The study examines the extent and frequency of a knockdown-type resistance allele (kdr type) in North American populations human head lice. Lice were collected from 32 locations Canada United States. DNA was extracted individual lice used to determine their zygosity using serial invasive signal amplification technique detect kdr-type T917I (TI) mutation, which is most responsible for nerve insensitivity that results kdr phenotype permethrin resistance. Previously sampled sites resampled if...
A quantitative sequencing (QS) protocol that detects the frequencies of sodium channel mutations (M815I, T917I, and L920F) responsible for knockdown resistance in permethrin-resistant head lice (Pediculus humanus capitis De Geer) was tested as a population genotyping method use preliminary monitoring tool. Genomic DNA fragments α-subunit gene encompass three mutation sites were polymerase chain reaction (PCR)-1 amplified from individual with either resistant or susceptible genotypes,...
Two point mutations (V419L and L925I) in the voltage-sensitive sodium channel alpha-subunit gene have been identified deltamethrin-resistant bed bugs. A quantitative sequencing (QS) protocol was developed to establish a population-based genotyping method as molecular resistance-monitoring tool based on frequency of two mutations. The nucleotide signal ratio at each mutation site generated from chromatograms plotted against corresponding resistance allele frequency. Frequency prediction...
Abstract Two point mutations (V419L and L925I) in the voltage-sensitive sodium channel α-subunit gene have been identified deltamethrin-resistant bed bugs. A quantitative sequencing (QS) protocol was developed to establish a population-based genotyping method as molecular resistance-monitoring tool based on frequency of two mutations. The nucleotide signal ratio at each mutation site generated from chromatograms plotted against corresponding resistance allele frequency. Frequency prediction...
Abstract We identified and characterized the full‐length cDNA sequences encoding two acetylcholinesterases (ClAChE1 ClAChE2) a salivary gland‐specific cholinesterase‐like protein (ClSChE) from common bed bug, Cimex lectularius . All three cholinesterase genes ( Clac1 , Clace2 Clsce ) have conserved motifs, including catalytic triad, choline‐binding site an acyl pocket. Phylogenetic analysis showed that ClAChE1 belongs to insect AChE1 clade, whereas ClAChE2 AChE2 clade. ClSChE was grouped...
A quantitative sequencing (QS) protocol that detects the frequencies of sodium channel mutations (M815I, T917I, and L920F) responsible for knockdown resistance in permethrin-resistant head lice (Pediculus humanus capitis De Geer) was tested as a population genotyping method use preliminary monitoring tool. Genomic DNA fragments α-subunit gene encompass three mutation sites were polymerase chain reaction (PCR)-1 amplified from individual with either resistant or susceptible genotypes,...