A5101555150- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Insect Pest Control Strategies
- Genetically Modified Organisms Research
- Virus-based gene therapy research
- Insect and Arachnid Ecology and Behavior
- Insect Utilization and Effects
- Bacteriophages and microbial interactions
- Cholinesterase and Neurodegenerative Diseases
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Viral Infections and Vectors
- RNA and protein synthesis mechanisms
- Invertebrate Immune Response Mechanisms
- Protein purification and stability
- Animal Disease Management and Epidemiology
- Plant Genetic and Mutation Studies
- Plant and animal studies
- Animal Virus Infections Studies
- Bacillus and Francisella bacterial research
Seoul National University
2014-2024
Gachon University
2024
Animal and Plant Quarantine Agency
2018
Ewha Womans University
1988
The impact of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) infection on host gene expression in Spodoptera exigua 4th instar larvae was investigated through the use 454 sequencing-based RNA-seq cDNA libraries developed from insects challenged with active AcMNPV or heat-inactivated AcMNPV.By comparing two libraries, we show that 201 genes are significantly up-regulated and 234 down-regulated by infection. Down-regulated included encoding antimicrobial peptides, namely...
Abstract Insect growth regulators (IGRs) are effective alternatives to chemical insecticides because of their specificity and low environmental toxicity. Entomopathogenic fungi an important natural pathogen insects have been developed as biological control agents. They produce a wide range secondary metabolites such antibiotics, pesticides, growth‐promoting or inhibiting compounds insect attracting In this study, explore novel IGR substances from entomopathogenic fungi, culture extracts 189...
ABSTRACT ORF78 ( ac78 ) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a baculovirus core gene unknown function. To determine the role in life cycle, an AcMNPV mutant with deleted, Ac78KO, was constructed. Quantitative PCR analysis revealed that late viral cycle. After transfection into Spodoptera frugiperda cells, Ac78KO produced single-cell infection phenotype, indicating no infectious budded viruses (BVs) were produced. The defect BV production also confirmed by both...
Two acetylcholinesterases (AChEs; BgAChE1 and BgAChE2) from Blattella germanica were functionally expressed using the baculovirus system. Kinetic analysis demonstrated that BgAChE2 had higher catalytic efficiency but lower substrate specificity than BgAChE1. With exceptions of paraoxon propoxur, was generally less sensitive to inhibitors BgAChE2. Western blot anti-BgAChE antibodies revealed far more abundant in all examined tissues compared BgAChE2, which is only present central nervous Both...
ABSTRACT A novel recombinant baculovirus, NeuroBactrus, was constructed to develop an improved baculovirus insecticide with additional beneficial properties, such as a higher insecticidal activity and recovery, compared wild-type baculovirus. For the construction of Bacillus thuringiensis crystal protein gene (here termed cry1-5 ) introduced into Autographa californica nucleopolyhedrovirus (AcMNPV) genome by fusion polyhedrin– –polyhedrin genes under control polyhedrin promoter. In opposite...
Abstract We investigated the occurrence of entomopathogenic fungi in 1080 soil samples representing multiple locations and conditions Korea. Entomopathogenic were isolated from soils using a selective medium containing dodine antibiotics. Following an initial identification based on morphology, fungal isolates more precisely identified by sequence their nuclear ribosomal RNA (rRNA) internal transcribed spacer (ITS) regions. As result, found to occur 32% (342 isolates) studied. The most...
Background Spodoptera litura is a noctuid moth that considered an agricultural pest. The larvae feed on wide range of plants and have been recorded from 40 plant families (mostly dicotyledons). It major pest many crops. To better understand granulovirus (SpliGV), the nucleotide sequence SpliGV DNA genome was determined analyzed. Methodology/Principal Findings completely sequenced. 124,121 bp long with 61.2% A+T content contained 133 putative open reading frames (ORFs) 150 or more...
Hepatitis C virus (HCV) is a pathogen that causes cirrhosis and hepatocellular carcinoma through chronic hepatitis C. This study focused on the anti-HCV activity of 70% ethanol extract
ABSTRACT ORF11 ( ac11 ) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved gene with unknown function. To determine the role in baculovirus life cycle, an knockout mutant AcMNPV, Ac11KO, was constructed. Northern blot and 5′ rapid amplification cDNA ends (RACE) analyses revealed that early cycle. Microscopy, titration assays, Western analysis budded viruses (BVs) were not produced Ac11KO-transfected Sf9 cells. However, quantitative PCR (qPCR) demonstrated...