A5101498213- Soybean genetics and cultivation
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Plant pathogens and resistance mechanisms
- Lipid metabolism and biosynthesis
- Phytoestrogen effects and research
- Plant Pathogenic Bacteria Studies
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Ubiquitin and proteasome pathways
- Plant biochemistry and biosynthesis
- Plant Parasitism and Resistance
- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Plant responses to water stress
- Garlic and Onion Studies
- Food composition and properties
- Agronomic Practices and Intercropping Systems
- Plant Genetic and Mutation Studies
- Seed Germination and Physiology
Kyungpook National University
2016-2025
Daegu University
2017
Qilu Hospital of Shandong University
2015
Chonnam National University
2004
University of Chicago
2003-2004
Seoul National University
1996-2002
Methyl jasmonate is a plant volatile that acts as an important cellular regulator mediating diverse developmental processes and defense responses. We have cloned the novel gene JMT encoding S-adenosyl-l-methionine:jasmonic acid carboxyl methyltransferase (JMT) from Arabidopsis thaliana. Recombinant protein expressed in Escherichia coli catalyzed formation of methyl jasmonic with K(m) value 38.5 microM. RNA was not detected young seedlings but rosettes, cauline leaves, developing flowers. In...
The balance between the bioactive sphingolipid ceramide and its phosphorylated derivative has been proposed to modulate amount of programmed cell death (PCD) in eukaryotes. We characterized first kinase (CERK) mutant any organism. Arabidopsis CERK mutant, called accelerated 5 , accumulates substrates shows enhanced disease symptoms during pathogen attack apoptotic-like dependent on defense signaling late development. ACD5 protein high specificity for ceramides vitro. Strikingly, C2 induces,...
Summary The role of A t MYB 44 , an R 2 3 transcription factor, in signaling mediated by jasmonic acid ( JA ) and salicylic SA is examined. induced through CORONATINE INSENSITIVE 1 COI 1). over‐expression down‐regulated defense responses against the necrotrophic pathogen lternaria brassicicola but up‐regulated WRKY 70 PR genes, leading to enhanced resistance biotrophic P seudomonas syringae pv. tomato DC 3000. knockout mutant atmyb44 shows opposite effects. Induction reduced npr1 ‐ mutants,...
The Arabidopsis thaliana agd2-like defense response protein1 (ald1) mutant was previously found to be hypersusceptible the virulent bacterial pathogen Pseudomonas syringae and had reduced accumulation of signal salicylic acid (SA). ALD1 shown possess aminotransferase activity in vitro, suggesting it generates an amino acid-derived signal. We now find a key component that acts multiple contexts partially requires PHYTOALEXIN DEFICIENT4 (PAD4) regulatory gene for its expression infection. ald1...
The disease-resistant Arabidopsis thaliana aberrant growth and death2 (agd2-1) mutant has elevated levels of the defense signal salicylic acid (SA), altered leaf morphology, mild dwarfism. AGD2 its close homolog ALD1 (for AGD2-LIKE DEFENSE RESPONSE PROTEIN1) encode aminotransferases that act on an overlapping set amino acids in vitro. However, kinetic parameters indicate may drive aminotransferase reaction opposite directions. ALD1-deficient mutants have phenotypes from agd2-1, showing...
Ethylene-responsive factors (ERFs), within a subgroup of the AP2/ERF transcription factor family, are involved in diverse plant reactions to biotic or abiotic stresses. Here, we report that overexpression an ERF gene from Brassica rapa ssp. pekinensis (BrERF4) led improved tolerance salt and drought stresses Arabidopsis. It also significantly affected growth development transgenic plants. We detected salt-induced expressions transcriptional repressor gene, AtERF4, some Ser/Thr protein...
We investigated the biological roles of Arabidopsis (Arabidopsis thaliana) GROWTH-REGULATING FACTOR (GRF) and GRF-INTERACTING (GIF) transcriptional complex in development gynoecia anthers. There are nine GRFs three GIFs Arabidopsis, seven posttranscriptionally silenced by microRNA396 (miR396). found that overexpression MIR396 gif1 gif2 double mutant background (gif1 35S:MIR396) resulted neither ovary nor pollen. Histological molecular marker-based analyses revealed gynoecial primordia failed...
Seed size is one of the most important traits determining yield cereal crops. Many studies have been performed to uncover mechanism seed development. However, much remains be understood, especially at molecular level, although several genes involved in identified. Here, we show that rice Grain Width 2 (GW2), a RING-type E3 ubiquitin ligase, can control development by catalyzing ubiquitination expansin-like 1 (EXPLA1), cell wall-loosening protein increases growth. Microscopic examination...
Renewable energy generation has attracted growing interest globally. The agro-photovoltaic (APV) system is a new alternative to conventional photovoltaic power plants, which can simultaneously generate renewable and increase agricultural productivity by the use of solar panels on same farmland. optimization crop yields assessment their environmental sensitivity under have not yet been evaluated with various species. This study aimed evaluate agronomic performances APV open field species such...
Drought is a significant abiotic stress that limits crop production. Soybeans [Glycine max (L.) Merr.] are regarded as drought-sensitive. In the present study, drought responses of wild soybean accessions were evaluated at different growth stages. Based on leaf wilting index 411 vegetative stage, seven highly tolerant (HT) and 24 (T) identified, although most soybeans classified moderate (M), sensitive (S), (HS) genotypes. addition, with selected soybeans, stomatal density decreased in HT...
To test the effect of physical proximity two enzymes catalyzing sequential reactions, a bifunctional fusion enzyme, TPSP, was constructed by fusing Escherichia coli genes for trehalose-6-phosphate (T6P) synthetase (TPS) and phosphatase (TPP). TPSP catalyzes reaction in which T6P is formed then dephosphorylated, leading to synthesis trehalose. The fused chimeric gene overexpressed E. purified near homogeneity; its molecular weight 88,300, as expected. K(m) values enzyme overall from...
Photoreceptors perceive different wavelengths of light and transduce signals downstream via a range proteins. COP1, an E3 ubiquitin ligase, regulates signaling by mediating the ubiquitination subsequent proteasomal degradation photoreceptors such as phytochromes cryptochromes, well various development-related proteins including other light-responsive COP1 is itself regulated direct interactions with several molecules that modulate its activity. The control photomorphogenesis also...
Soybean is particularly known for accumulating saponins in its seeds. This study aimed to identify a causal gene control an increase Ab-γg saponin PE1607 from EMS-treated population of the soybean cultivar Pungsannamul. Segregation analysis F2 seeds verified that single recessive allele controlled increased PE1607. Bulk segregant and mutant individuals identified candidate region, containing previously reported Sg-3 (Glyma.10G104700) gene, encoding glucosyltransferase responsible conjugating...
Endogenous salicylic acid (SA) and its predominant conjugates, SA 2-O-β-D-glucoside (SAG) the glucose ester of (SGE), increase dramatically during plant defense responses. Here I report isolation characterization an Arabidopsis thaliana UDP- glucosetSA glucosyltransferase1 (AtSGT1) gene using a tobacco SGT previously reported, whose product catalyzes formation both SAG SGE. The recombinant AtSGT1 protein had significant activities with benzoic acid, synthesized Northern blot analysis showed...
Protein disulfide isomerase (PDI) is a chaperone protein involved in oxidative folding by acting as catalyst and assisting the endoplasmic reticulum (ER). A genome database search showed that rice contains 19 PDI-like genes. However, their functions are not clearly identified. This paper shows possible of 1-1 (PDIL1-1) during seed development. Seeds T-DNA insertion PDIL1-1 mutant, PDIL1-1Δ, identified genomic DNA PCR western blot analysis, display chalky phenotype thick aleurone layer....
Soybeans (Glycine max (L.) Merr.) with black seed coats and green cotyledons are rich in anthocyanins chlorophylls known as functional nutrients, antioxidants compounds anticarcinogenic properties. Understanding the genetic diversity of germplasm is important to determine effective strategies for improving economic traits these soybeans. We aimed analyze 470 soybean accessions by 6K single nucleotide polymorphic loci architecture soybeans cotyledons. found showed narrow variability South...