Abstract AtMYB44 belongs to the R2R3 MYB subgroup 22 transcription factor family in Arabidopsis (Arabidopsis thaliana). Treatment with abscisic acid (ABA) induced transcript accumulation within 30 min. The gene was also activated under various abiotic stresses, such as dehydration, low temperature, and salinity. In transgenic carrying an promoter-driven β-glucuronidase (GUS) construct, strong GUS activity observed vasculature leaf epidermal guard cells. Transgenic overexpressing is more...

Abstract Rice (Oryza sativa), a monocotyledonous plant that does not cold acclimate, has evolved differently from Arabidopsis (Arabidopsis thaliana), which acclimates. To understand the stress response of rice in comparison with Arabidopsis, we developed transgenic plants constitutively expressed CBF3/DREB1A (CBF3) and ABF3, genes function abscisic acid-independent acid-dependent stress-response pathways, respectively. CBF3 elevated tolerance to drought high salinity, produced relatively low...

Summary Jasmonates play important roles in development, stress responses and defense plants. Here, we report the results of a study using functional genomics approach that identified rice basic helix‐loop‐helix domain gene, OsbHLH148 , conferred drought tolerance as component jasmonate signaling module rice. transcript levels were rapidly increased by treatment with methyl (MeJA) or abscisic acid, abiotic stresses including dehydration, high salinity, low temperature wounding. Transgenic...

Abstract Trehalose plays an important role in stress tolerance plants. Trehalose-producing, transgenic rice (Oryza sativa) plants were generated by the introduction of a gene encoding bifunctional fusion (TPSP) trehalose-6-phosphate (T-6-P) synthase (TPS) and T-6-P phosphatase (TPP) ofEscherichia coli, under control maize (Zea mays) ubiquitin promoter (Ubi1). The high catalytic efficiency (Seo et al., 2000) enzyme single-gene engineering strategy make this attractive candidate for high-level...

Abstract The rice (Oryza sativa) spotted leaf11 (spl11) mutant was identified from an ethyl methanesulfonate–mutagenized indica cultivar IR68 population and previously shown to display a spontaneous cell death phenotype enhanced resistance fungal bacterial pathogens. Here, we have isolated Spl11 via map-based cloning strategy. isolation of the gene facilitated by identification three additional spl11 alleles IR64 collection. predicted SPL11 protein contains both U-box domain armadillo (ARM)...

Abstract The tapetum, the innermost of four sporophytic layers in anther wall, comes direct contact with developing male gametophyte and is thought to play a crucial role development maturation microspores. Here, we report identification rice (Oryza sativa) Undeveloped Tapetum1 (Udt1), which required for differentiation secondary parietal cells mature tapetal cells. T-DNA or retrotransposon Tos17 insertions Udt1 gene caused sterility. walls meiocytes mutants were normal during early...

Ethylene-responsive factors (ERFs), within a subgroup of the AP2/ERF transcription factor family, are involved in diverse plant reactions to biotic or abiotic stresses. Here, we report that overexpression an ERF gene from Brassica rapa ssp. pekinensis (BrERF4) led improved tolerance salt and drought stresses Arabidopsis. It also significantly affected growth development transgenic plants. We detected salt-induced expressions transcriptional repressor gene, AtERF4, some Ser/Thr protein...

The expression of the six rice ASR genes is differentially regulated in a tissue-dependent manner according to environmental conditions and reproductive stages. OsASR1 OsASR3 are most abundant found tissues; they enriched leaves roots, respectively. Coexpression analysis comparison cis-acting elements upstream suggested that their common by abiotic stresses but differently hormone sugar signals. results quantitative real-time PCR analyses under various further support this model. both was...

Plants have evolved strategies to cope with drought stress by maximizing physiological capacity and adjusting developmental processes such as flowering time. The WOX13 orthologous group is the most conserved among clade of WOX homeodomain-containing proteins found function in both flower development. In this study, we isolated characterized

Abstract Expression patterns of a rice (Oryza sativa) cytochrome c gene OsCc1 and its promoter activity were characterized in transgenic plants.OsCc1 transcripts accumulate most cell types, but to varying levels. Large amounts are found the roots, calli, suspension cells, relatively lower mature leaves, demonstrating higher levels expression non-photosynthetic tissues. Unlike human cytochromec gene, which is responsive cAMP,OsCc1 not enhanced various tissues after dibutyryl cAMP treatments....

Abstract Microarray data can be used to derive understanding of the relationships between genes involved in various biological systems an organism, given availability databases gene expression measurements from complete spectrum experimental conditions and materials. However, there have been no reports, date, such a database being constructed for rice (Oryza sativa). Here, we describe construction database, called RiceArrayNet (RAN; http://www.ggbio.com/arraynet/), which provides information...

We have previously isolated a rice gene encoding histone deacetylase, OsHDAC1, and observed that its transgenic overexpression increases seedling root growth. To identify the transcriptional repression events occur as result of OsHDAC1 (OsHDAC1(OE)), global profiling root-expressed genes was performed on OsHDAC1(OE) or HDAC inhibitor-treated non-transgenic (NT) roots, in comparison with untreated NT roots. selected 39 are induced repressed compared respectively. Interestingly, OsNAC6, member...

Agrobacterium radiobacter K84, used worldwide to biocontrol crown gall disease caused by tumefaciens , produces an antiagrobacterial compound called agrocin 84. We report the nucleotide sequence of pAgK84, a 44.42-kb plasmid coding for production this disubstituted adenine antibiotic. pAgK84 encodes 36 ORFs, 17 which ( agn ) code synthesis or immunity Two genes, agnB2 and agnA encode aminoacyl tRNA synthetase homologues. have shown that toxic moiety 84 inhibits cellular leucyl-tRNA...

ABSTRACT Using an extracellular β‐fructosidase, inulase, which hydrolyzes β‐2: 1′‐linked fructose polymers, 90% of the total sugar in extract Jerusalem artichoke tubers were hydrolyzed to free sugars consisted 77% and 23% glucose. Fructose hydrolyzate was separated by Dowex 1‐X8 ion exchange chromatography obtained with overall recovery 61%.

ABSTRACT Burkholderia glumae is the causative agent of grain and seedling rot in rice bacterial wilt many field crops. Here, we report complete genome sequence B. BGR1 isolated from a diseased panicle Korea.

Summary Histone deacetylases (HDACs) modulate chromatin structure and transcription. HDACs have been studied as negative regulators in eukaryotic We isolated the rice OsHDAC1 – 3 genes for class I‐type histone deacetylases, which are related to RPD3 family. The gene encoded a protein of approximately 57 kDa that shared 73.5, 72.7, 79.9, 57.1% amino acid sequence identity with OsHDAC2, OsHDAC3, maize RPD3, human HDAC1 proteins, respectively. Genomic structures Southern blot analyses revealed...

Two global approaches were applied to develop an inventory of differentially expressed proteins and genes in rice (cv. Nipponbare) seedling grown on Murashige Skoog medium with without jasmonic acid (JA). JA significantly reduced the growth shoot, root, leaf, leaf sheath depending concentration (1, 2, 5, 10, 25, 50 μM) as compared control. Almost 50% inhibition was observed 5 μM JA. Shoots roots seedlings for 7 days then used proteomics transcriptomics analyses. Two-dimensional gel...

Abstract Background Water deficiency is one of the most serious worldwide problems for agriculture. Recently, it has become more and outspread, which urgently requires production drought-tolerant plants. Microarray experiments using mRNA from air-dried leaves roots rice were performed in an attempt to study genes involved acute dehydration response. Results Set 10,537 was significantly up- or down-regulated under treatment. Gene Ontology analysis highlighted gene expression during response...

Background The perturbation of the steady state reactive oxygen species (ROS) due to biotic and abiotic stresses in a plant could lead protein denaturation through modification amino acid residues, including oxidation methionine residues. Methionine sulfoxide reductases (MSRs) catalyze reduction back residue. To assess role this enzyme, we generated transgenic rice using pepper CaMSRB2 gene under control Rab21 (responsive ABA 21) promoter with/without selection marker, bar gene. Results A...

Histone deacetylases (HDACs) are known to function in the nucleus. Here, we report on organellar localization of three rice HDACs, OsSIR2b, OsHDAC6, and OsHDAC10. The 35S:OsSIR2b-GFP 35S:OsHDAC10-GFP constructs were introduced into tobacco BY2 cells. Co-localization analysis green fluorescent protein MitoTracker signals transformed cells indicated that OsSIR2b OsHDAC10 localized mitochondria. Transgenic Arabidopsis lines harboring 35S:OsHDAC6-GFP similarly analyzed, revealing OsHDAC6-GFP is...

Spatial- and temporal-specific expression patterns are primarily regulated at the transcriptional level by gene promoters. Therefore, it is important to identify binding motifs of transcription factors better understand networks associated with embryogenesis. Here, we used a protein-binding microarray (PBM) OsSMF1, which basic leucine zipper factor involved in regulation rice seed maturation. OsSMF1 (previously called RISBZ1 or OsbZIP58) known interact GCN4 (TGA(G/C)TCA) regulate storage...