A5090190500- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Insect-Plant Interactions and Control
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- RNA Research and Splicing
- Plant Reproductive Biology
- Enzyme Production and Characterization
- Legume Nitrogen Fixing Symbiosis
- Biofuel production and bioconversion
- Garlic and Onion Studies
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Insect and Pesticide Research
- Soybean genetics and cultivation
- Plant Disease Resistance and Genetics
Animal and Plant Quarantine Agency
2024
Seoul National University
2011-2023
National Academy of Sciences
2019-2022
Myongji University
2002-2012
Korea University
2009-2012
Rockefeller University
2012
University of California, Riverside
2012
RIKEN Advanced Science Institute
2012
National Academy of Agricultural Science
2010
Kyungpook National University
2009
Trehalose is a nonreducing disaccharide of glucose that functions as compatible solute in the stabilization biological structures under abiotic stress bacteria, fungi, and invertebrates. With notable exception desiccation-tolerant “resurrection plants,” trehalose not thought to accumulate detectable levels most plants. We report here regulated overexpression Escherichia coli biosynthetic genes ( otsA otsB ) fusion gene for manipulating tolerance rice. The has advantages necessitating only...
Doil Choi and colleagues report the genome sequence of hot pepper, Capsicum annuum, as well resequencing two cultivated peppers a wild species, chinense. Comparative genomic analysis across Solanaceae provides insights into expansion, pungency, ripening disease resistance in peppers. Hot pepper (Capsicum annuum), one oldest domesticated crops Americas, is most widely grown spice crop world. We whole-genome sequencing assembly (Mexican landrace annuum cv. CM334) at 186.6× coverage. also de...
Abstract Drought poses a serious threat to the sustainability of rice (Oryza sativa) yields in rain-fed agriculture. Here, we report results functional genomics approach that identified NAC (an acronym for NAM [No Apical Meristem], ATAF1-2, and CUC2 [Cup-Shaped Cotyledon]) domain gene, OsNAC10, which improved performance transgenic plants under field drought conditions. Of 140 OsNAC genes predicted rice, 18 were be induced by stress Phylogenic analysis revealed presence three subgroups with...
Methyl jasmonate is a plant volatile that acts as an important cellular regulator mediating diverse developmental processes and defense responses. We have cloned the novel gene JMT encoding S-adenosyl-l-methionine:jasmonic acid carboxyl methyltransferase (JMT) from Arabidopsis thaliana. Recombinant protein expressed in Escherichia coli catalyzed formation of methyl jasmonic with K(m) value 38.5 microM. RNA was not detected young seedlings but rosettes, cauline leaves, developing flowers. In...
Abstract AtMYB44 belongs to the R2R3 MYB subgroup 22 transcription factor family in Arabidopsis (Arabidopsis thaliana). Treatment with abscisic acid (ABA) induced transcript accumulation within 30 min. The gene was also activated under various abiotic stresses, such as dehydration, low temperature, and salinity. In transgenic carrying an promoter-driven β-glucuronidase (GUS) construct, strong GUS activity observed vasculature leaf epidermal guard cells. Transgenic overexpressing is more...
Summary Jasmonates play important roles in development, stress responses and defense plants. Here, we report the results of a study using functional genomics approach that identified rice basic helix‐loop‐helix domain gene, OsbHLH148 , conferred drought tolerance as component jasmonate signaling module rice. transcript levels were rapidly increased by treatment with methyl (MeJA) or abscisic acid, abiotic stresses including dehydration, high salinity, low temperature wounding. Transgenic...
Abstract Trehalose plays an important role in stress tolerance plants. Trehalose-producing, transgenic rice (Oryza sativa) plants were generated by the introduction of a gene encoding bifunctional fusion (TPSP) trehalose-6-phosphate (T-6-P) synthase (TPS) and T-6-P phosphatase (TPP) ofEscherichia coli, under control maize (Zea mays) ubiquitin promoter (Ubi1). The high catalytic efficiency (Seo et al., 2000) enzyme single-gene engineering strategy make this attractive candidate for high-level...
Heterogeneous nuclear RNA-ribonucleoprotein (hnRNP) particles can be efficiently purified by a specific, rapid, and mild procedure using monoclonal antibodies to hnRNP proteins. We report here on the detailed analysis of protein composition immunopurified from human HeLa cells. By two-dimensional gel electrophoresis, contain at least 24 polypeptides in range 34,000-120,000 daltons. The abundant 30,000-40,000 dalton proteins, A, B, C, described previously, are subset these polypeptides....
Splicing in vitro of a messenger RNA (mRNA) precursor (pre-mRNA) is inhibited by monoclonal antibody to the C proteins (anti-C) heterogeneous nuclear (hnRNA)-ribonucleoprotein (hnRNP) particles. This antibody, 4F4, inhibits an early step reaction: cleavage at 3′ end upstream exon and formation intron lariat. In contrast, boiled or different (designated 2B12) proteins, antibodies other hnRNP (120 68 kilodaltons) nonimmune mouse have no inhibitory effect. The 4F4 does not prevent adenosine...
Summary Drought conditions are among the most serious challenges to crop production worldwide. Here, we report results of field evaluations transgenic rice plants overexpressing OsNAC5 , under control either root‐specific ( RCc3 ) or constitutive GOS2 promoters. Field over three growing seasons revealed that grain yield RCc3:OsNAC5 and GOS2:OsNAC5 were increased by 9%–23% 9%–26% normal conditions, respectively. Under drought however, showed a significantly higher 22%–63%, whilst reduced...
Exposure of intact cells to UV light brings about cross-linking polyadenylated mRNA a set cytoplasmic proteins which are in direct contact with the vivo. Substantial amounts an additional protein molecular weight 38,000 (38K) become cross-linked when treated inhibitors synthesis (actinomycin D, camptothecin, and 5,6-dichloro-1-beta-D-ribofuranosyl benzimidazole) or after infection vesicular stomatitis virus. Cordycepin, inhibits polyadenylation but not synthesis, has no such effect....
In Arabidopsis, jasmonate is required for stamen and pollen maturation. Mutants deficient in synthesis, such as opr3, are male-sterile but become fertile when applied to developing flower buds. We have used ATH1 oligonucleotide arrays follow gene expression opr3 stamens 22 h following treatment. these experiments, a total of 821 genes were specifically induced by 480 repressed. Comparisons with data from previous studies indicate that constitute stamen-specific transcriptome, large...
Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins are harmful to animal and human. In this study, a systematic analysis 17 phenotypes the mutants in 657 genes encoding putative transcription factors (TFs) resulted database over 11,000 (phenome). This provides comprehensive insights into how global significance regulates traits for growth, development, stress response, pathogenesis, toxin production...
Drought conditions limit agricultural production by preventing crops from reaching their genetically predetermined maximum yields. Here, we present the results of field evaluations rice overexpressing OsNAC9, a member NAC domain family. Root-specific (RCc3) and constitutive (GOS2) promoters were used to overexpress OsNAC9 produced transgenic RCc3:OsNAC9 GOS2:OsNAC9 plants. Field over two cultivating seasons showed that grain yields plants increased 13%-18% 13%-32% under normal conditions,...
Plants have evolved to sophisticated adaptation mechanisms cope with drought stress by reprograming transcriptional networks through responsive transcription factors. NAM, ATAF1-2, and CUC2 (NAC) factors are known be associated various developmental processes tolerance. In this study, we functionally characterized the rice factor OsNAC14. OsNAC14 was predominantly expressed at meiosis stage but is induced drought, high salinity, ABA low temperature in leaves. Overexpression of resulted...
Plant responses to drought stress require the regulation of transcriptional networks via drought-responsive transcription factors, which mediate a range morphological and physiological changes. AP2/ERF factors are known act as key regulators resistance networks; however, little is about associated molecular mechanisms that give rise specific adaptations. In this study, we functionally characterized rice (Oryza sativa) factor OsERF71, expressed predominantly in root meristem, pericycle,...
Summary Variegated plants have green‐ and white‐sectored leaves. Cells in the green sectors contain morphologically normal chloroplasts, whereas cells white non‐pigmented plastids that lack organized lamellar structures. Many variegations are caused by mutations nuclear genes affect plastid function, yet only a few cases responsible been cloned. We show VAR 2 locus of Arabidopsis cause variegation due to loss chloroplast thylakoid membrane protein bears similarity FtsH family AAA proteins...
Jasmonic acid (JA) is involved in plant development and the defense response. Transgenic overexpression of Arabidopsis (Arabidopsis thaliana) jasmonic carboxyl methyltransferase gene (AtJMT) linked to Ubi1 promoter increased levels methyl jasmonate (MeJA) by 6-fold young panicles. Grain yield was greatly reduced Ubi1:AtJMT plants due a lower numbers spikelets filling rates than were observed for nontransgenic (NT) controls. had altered spikelet organs, including lemma/palea, lodicule,...
Exposure of cells to UV light sufficient intensity brings about cross-linking RNA proteins which are in direct contact with it vivo. The major [35S]methionine-labeled become cross-linked polyadenylated heterogeneous nuclear HeLa have molecular weights 120,000 (120K), 68K, 53K, 43K, 41K, 38K, and 36K. Purified complexes obtained by intact were used immunize mice generate monoclonal antibodies several these proteins. Some properties three the proteins, 120K, characterized antibodies. 41K 43K...
The C proteins (C1 and C2) are major constituents of the 40S subparticle heterogeneous nuclear ribonucleoprotein complexes (hnRNPs) (Beyer, A.L., M.E. Christensen, B.W. Walker, W.M. LeStourgeon, 1977, Cell, 11:127-138) two most prominent that become cross-linked by ultraviolet light to RNA (hnRNA) in vivo. Studies described here on characterization vertebrate cells using monoclonal polyclonal antibodies. Monoclonal antibodies genuine RNP proteins, including were obtained immunizing mice with...
Summary The role of A t MYB 44 , an R 2 3 transcription factor, in signaling mediated by jasmonic acid ( JA ) and salicylic SA is examined. induced through CORONATINE INSENSITIVE 1 COI 1). over‐expression down‐regulated defense responses against the necrotrophic pathogen lternaria brassicicola but up‐regulated WRKY 70 PR genes, leading to enhanced resistance biotrophic P seudomonas syringae pv. tomato DC 3000. knockout mutant atmyb44 shows opposite effects. Induction reduced npr1 ‐ mutants,...
The packaging of heterogeneous nuclear RNA (hnRNA), the fate hnRNA in nucleus, and conversion to mRNA are believed occur as transcript is associated with specific proteins form a ribonucleoprotein complex termed hnRNP complex. identity organization protein constituents have been matter considerable controversy. We report here isolation from vertebrate cell nuclei, employing immunoprecipitation monoclonal antibodies against major that contact HeLa cells. Rapid nucleoplasm two different C (41...