A5058486630- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- Fungal Biology and Applications
- Mycorrhizal Fungi and Plant Interactions
- Plant-Microbe Interactions and Immunity
- Plant and fungal interactions
- Microbial Natural Products and Biosynthesis
- Lichen and fungal ecology
- Wheat and Barley Genetics and Pathology
- Plant Reproductive Biology
- Yeasts and Rust Fungi Studies
- Genomics and Phylogenetic Studies
- Biofuel production and bioconversion
- Plant and Fungal Interactions Research
- Biocrusts and Microbial Ecology
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Biochemical and Structural Characterization
- Slime Mold and Myxomycetes Research
- Korean Urban and Social Studies
- Evolution and Genetic Dynamics
- Bryophyte Studies and Records
- Enzyme-mediated dye degradation
Republic of Korea Army
2025
Soonchunhyang University
2014-2024
Jeonbuk National University
2016
Rural Development Administration
2012
National Academy of Agricultural Science
2012
Cornell University
1998-2004
Syngenta (United States)
2002
Torrey Pines Institute For Molecular Studies
2002
University of British Columbia
1999
Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins are harmful to animal and human. In this study, a systematic analysis 17 phenotypes the mutants in 657 genes encoding putative transcription factors (TFs) resulted database over 11,000 (phenome). This provides comprehensive insights into how global significance regulates traits for growth, development, stress response, pathogenesis, toxin production...
ABSTRACT Gibberella zeae , a major cause of cereal scab, can be divided into two chemotypes based on production the 8-ketotrichothecenes deoxynivalenol (DON) and nivalenol (NIV). We cloned sequenced Tri13 homolog from each chemotype. The NIV chemotype strain (88-1) is located in trichothecene gene cluster carries an open reading frame similar to that Fusarium sporotrichioides whereas DON (H-11) several mutations. To confirm roles Tri7 genes by G. we genetically altered toxin 88-1 H-11. In...
In most fungal ascomycetes, mating is controlled by a single locus ( MAT ). Fungi requiring partner to mate are heterothallic (self-sterile); those not homothallic (self-fertile). Structural analyses of sequences from and Cochliobolus species support the hypothesis that heterothallism ancestral. Homothallic carry both genes in nucleus, usually closely linked or fused, contrast species, which have alternate different nuclei. The structural organization all examined highly conserved; contrast,...
Summary Zearalenone (ZEA) is a polyketide mycotoxin produced by some species of Gibberella/Fusarium and causes hyperestrogenic syndrome in animals. ZEA occurs naturally cereals infected Gibberella zeae temperate regions threatens animal health. In this study, we report on set genes that participate the biosynthesis G. . Focusing non‐reducing synthase (PKS) genome, demonstrated PKS13 required for production. Subsequent analyses revealed continuous, 50 kb segment DNA carrying consisted three...
ABSTRACT Double-stranded RNA (dsRNA) viruses in some fungi are associated with hypovirulence and have been used or proposed as biological control agents. We isolated 7.5-kb dsRNAs from 13 of 286 field strains Fusarium graminearum maize Korea. One these strains, DK21, was examined more detail. This strain had pronounced morphological changes, including reduction mycelial growth, increased pigmentation, reduced virulence towards wheat, decreased (60-fold) production trichothecene mycotoxins....
Fusarium graminearum is an important fungal pathogen of cereal crops and produces mycotoxins, such as the trichothecenes nivalenol deoxynivalenol. This species may be subdivided into a series genetic lineages or phylogenetic species. We identified strains F. from Republic Korea to lineage, tested their ability produce deoxynivalenol, determined composition structure populations which they were recovered. Based on amplified fragment length polymorphism (AFLP), PCR genotyping, chemical...
In fungi, genes involved in biosynthesis of a secondary metabolite (SM) are often located adjacent to one another the genome and coordinately regulated. These SM biosynthetic gene clusters typically encode enzymes, or more transcription factors, transport protein. Fusaric acid is polyketide-derived produced by multiple species fungal genus Fusarium. This concern because it toxic animals and, therefore, considered mycotoxin may contribute plant pathogenesis. Preliminary descriptions fusaric...
ABSTRACT Gibberella zeae , a major cause of cereal scab, may be divided into two chemotypes based on production the trichothecenes deoxynivalenol (DON) and nivalenol (NIV). We cloned sequenced gene cluster for trichothecene biosynthesis from each chemotype. G. H-11 is DON producer isolated corn, 88-1 NIV barley. 23-kb 26-kb 88-1, along with unlinked Tri101 genes. Each contained 10 Tri homologues in same order transcriptional directions as those Fusarium sporotrichioides . Between all except...
Summary Fungi capable of sexual reproduction use heterothallic (self‐sterile) or homothallic (self‐fertile) mating strategies. In most ascomycetes, a single type locus, MAT , with two alternative forms ( MAT1‐1 and MAT1‐2 ) called idiomorphs, controls ability. these idiomorphs reside in different nuclei. contrast, ascomycetes carry both nucleus, usually closely linked. An example the latter is Gibberella zeae species that selfing outcrossing. G. devastating cereal pathogen ubiquitous...
ABSTRACT Mycelia of Gibberella zeae (anamorph, Fusarium graminearum ), an important pathogen cereal crops, are yellow to tan with white carmine red margins. We isolated genes encoding the following two proteins that required for aurofusarin biosynthesis from G. : a type I polyketide synthase (PKS) and putative laccase. Screening insertional mutants , which were generated by using restriction enzyme-mediated integration procedure, resulted in isolation mutant S4B3076, is pigment mutant. In...
The homothallic ascomycete fungus Gibberella zeae (anamorph: Fusarium graminearum) is a major toxigenic plant pathogen that causes head blight disease on small-grain cereals. produces the mycotoxins deoxynivalenol (DON) and zearalenone (ZEA) in infected hosts, posing threat to human animal health. Despite its agricultural toxicological importance, molecular mechanisms underlying growth, development virulence remain largely unknown. To better understand such mechanisms, we studied...
Fusarium graminearum, the causal agent of head blight in cereal crops, produces sexual progeny (ascospore) as an important overwintering and dissemination strategy for completing disease cycle. This homothallic ascomycetous species does not require a partner mating; instead, it carries two opposite mating-type (MAT) loci single nucleus to control development. To gain comprehensive understanding regulation development F. we used in-depth high-throughput analyses examine target genes...
Fusarium fujikuroi causes bakanae ("foolish seedling") disease of rice which is characterized by hyper-elongation seedlings resulting from production gibberellic acids (GAs) the fungus. This plant pathogen also known for harmful mycotoxins, such as fusarins, fusaric acid, apicidin F and beauvericin. Recently, we generated first de novo genome sequence F. strain IMI 58289 combined with extensive transcriptional, epigenetic, proteomic chemical product analyses. GA was shown to provide a...
The depside and depsidone series compounds of polyketide origin accumulate in the cortical or medullary layers lichen thalli. Despite taxonomic ecological significance chemistry its pharmaceutical potentials, there has been no single piece genetic evidence linking biosynthetic genes to substances. Thus, we systematically analyzed synthases (PKSs) for categorization identification gene cluster (BGC) involved depside/depsidone production. Our in-depth analysis interspecies PKS diversity genus
Gibberella zeae is an ascomyceteous fungus that causes serious diseases in cereal crops. Severe epidemics require strains are virulent and can reproduce sexually. We characterized insertional mutant (designated ZH436) with a pleiotropic defect both traits, identified novel F-box protein gene encoding FBP1 (F-box 1) similar to fungal proteins including Saccharomyces cerevisiae Grr1, well-characterized component of the Skp1-Cullin-F-box (SCF(Grr1)) E3 ligase complex required for degradation....
Fusarium graminearum, the causal agent of head blight in cereal crops, produces mycotoxins such as trichothecenes and zearalenone infected plants. Here, we focused on function FgLaeA F. a homolog Aspergillus nidulans LaeA encoding global regulator for both secondary metabolism sexual development. Prior to gene analysis, constructed novel luciferase reporter system consisting transgenic graminearum strain expressing firefly under control promoter either TRI6 or ZEB2 controlling biosynthesis...
The velvet genes are conserved in ascomycetous fungi and function as global regulators of differentiation secondary metabolism. Here, we characterized one the genes, designated FgVelB, plant-pathogenic fungus Fusarium graminearum, which causes fusarium head blight cereals produces mycotoxins within plants. FgVelB-deleted (ΔFgVelB) strains produced fewer aerial mycelia with less pigmentation than those wild-type (WT) during vegetative growth. Under sexual development conditions, ΔFgVelB no...
ABSTRACT Gibberella zeae (anamorph: Fusarium graminearum ) is an important pathogen of maize, wheat, and rice. Colonies G. produce yellow-to-tan mycelia with the white-to-carmine red margins. In this study, we focused on nine putative open reading frames (ORFs) closely linked to PKS12 GIP1 , which are required for aurofusarin biosynthesis in . Among them ORF designated GIP2 (for Gi bberella p igment gene 2 ), encodes a protein 398 amino acids that carries Zn(II) Cys 6 binuclear cluster...
ABSTRACT The sucrose nonfermenting 1 ( SNF1 ) protein kinase of yeast plays a central role in the transcription glucose-repressible genes response to glucose starvation. In this study, we deleted an ortholog from Gibberella zeae characterize its functions by using gene replacement strategy. mycelial growth deletion mutants (ΔGz was reduced 21 74% on diverse carbon sources. virulence ΔGz barley decreased, and expression encoding cell-wall-degrading enzymes reduced. most distinct phenotypic...
ABSTRACT The glyoxylate and methylcitrate cycles are involved in the metabolism of two- or three-carbon compounds fungi. To elucidate role(s) these pathways Gibberella zeae , which causes head blight cereal crops, we focused on functions G. orthologs (Gz ICL 1 Gz MCL1 ) genes that encode isocitrate lyase (ICL) methylisocitrate (MCL), respectively, key enzymes each cycle. deletion ICL1 (ΔGz caused defects growth acetate perithecium (sexual fruiting body) formation but not virulence barley...
Summary Apicidin is a cyclic tetrapeptide produced by certain isolates of Fusarium semitectum and has been shown to inhibit Apicomplexan histone deacetylase. An apicidin‐producing strain (KCTC16676) the filamentous fungus was mutated using an Agrobacterium tumefaciens ‐mediated transformation, resulting in 24 apicidin‐deficient mutants. Three mutants had T‐DNA insertion gene that encodes non‐ribosomal peptide synthetase (NRPS). Results sequence, expression, deletion analyses defined apicidin...