A5090021726- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Transgenic Plants and Applications
- Plant and Fungal Interactions Research
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Plant Gene Expression Analysis
- Fungal and yeast genetics research
- Plant Pathogenic Bacteria Studies
- Anesthesia and Sedative Agents
- Video Analysis and Summarization
- Structural Analysis and Optimization
- Fungal Biology and Applications
- Composite Structure Analysis and Optimization
- Plant Stress Responses and Tolerance
- Anesthesia and Neurotoxicity Research
- Topology Optimization in Engineering
- Plant Parasitism and Resistance
- Multimedia Communication and Technology
- Ubiquitin and proteasome pathways
- Plant biochemistry and biosynthesis
- Legume Nitrogen Fixing Symbiosis
ZheJiang Academy of Agricultural Sciences
2009-2023
Second Affiliated Hospital of Zhejiang University
2022
Shandong Academy of Agricultural Sciences
2016
Institute of Virology of the Slovak Academy of Sciences
2014
The Ohio State University
2003-2009
University of California, Davis
2007
University of Arizona
2007
Abstract The broad-spectrum rice blast resistance gene Pi9 was cloned using a map-based cloning strategy. Sequencing of 76-kb bacterial artificial chromosome (BAC) contig spanning the locus led to identification six tandemly arranged resistance-like genes with nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) (Nbs1-Pi9–Nbs6-Pi9). Analysis selected deletion mutants transformation 45-kb fragment from BAC into susceptible cultivar TP309 narrowed down candidate Nbs2-Pi9 Nbs3-Pi9....
Abstract The rice (Oryza sativa) spotted leaf11 (spl11) mutant was identified from an ethyl methanesulfonate–mutagenized indica cultivar IR68 population and previously shown to display a spontaneous cell death phenotype enhanced resistance fungal bacterial pathogens. Here, we have isolated Spl11 via map-based cloning strategy. isolation of the gene facilitated by identification three additional spl11 alleles IR64 collection. predicted SPL11 protein contains both U-box domain armadillo (ARM)...
The rice blast resistance (R) genes Pi2 and Piz-t confer broad-spectrum against different sets of Magnaporthe grisea isolates. We first identified the gene using a map-based cloning strategy. is member cluster comprising nine members (named Nbs1-Pi2 to Nbs9-Pi2) encodes protein with nucleotide-binding site leucine-rich repeat (LRR) domain. Fine genetic mapping, molecular characterization susceptible mutants, complementation tests indicated that Nbs4-Pi2 gene. gene, allele in cultivar Toride...
Receptor-like kinases (RLKs) may initiate signaling pathways by perceiving and transmitting environmental signals to cellular machinery play diverse roles in plant development stress responses. The rice genome encodes more than one thousand RLKs, but only a small number have been characterized as receptors for phytohormones, polypeptides, elicitors, effectors. Here, we screened the function of 11 RLKs resistance blast fungus Magnaporthe oryzae (M. oryzae) identified negative regulator named...
Resistance in rice cultivars to the blast fungus Magnaporthe oryzae is complex and controlled by both major genes quantitative trait loci (QTLs). We undertook a genome-wide association study (GWAS) using diversity panel 1 (RDP1) that was genotyped high-density (700 000 single nucleotide polymorphisms) array inoculated with five diverse M. isolates. identified 97 associated resistance (LABRs). Among them, 82 were new regions 15 co-localized known loci. The top 72 LABRs explained up 98% of...
Rice blast disease is one of the most destructive rice diseases worldwide. The pi21 gene confers partial and durable resistance to Magnaporthe oryzae. However, little known regarding molecular mechanisms mediated by loss-of-function Pi21. In this study, comparative transcriptome profiling Pi21-RNAi transgenic line Nipponbare with M. oryzae infection at different time points (0, 12, 24, 48, 72 hpi) were investigated using RNA sequencing. results generated 43,222 unique genes mapped genome....
Abstract Transposon insertional mutagenesis is an effective alternative to T-DNA when transformation through tissue culture inefficient as the case for many crop species. When used activation tags, transposons can be exploited generate novel gain-of-function phenotypes without and are of particular value in study polyploid plants where gene knockouts will not have phenotypes. We developed cis-activation-tagging Ac-Ds transposon system which a vector carries Dissociation (Ds) element...
Abstract Background Long non-coding RNAs (LncRNAs) have emerged as important regulators in many physiological processes plant. By high-throughput RNA-sequencing, pathogen-associated LncRNAs were mapped various plants, and some of them proved to be involved plant defense responses. The rice blast disease caused by Magnaporthe oryzae ( M. ) is one the most destructive diseases rice. However, -induced yet studied. Findings We investigated that associated with fungus. Totally 83 up-regulated...
Marker-free transgenic plants can be developed through transposon-mediated transgene reintegration, which allows intact insertion with defined boundaries and requires only a few primary transformants. In this study, we improved the selection strategy validated that maize (Zea mays) Activator/Dissociation (Ds) transposable element routinely used to generate marker-free plants. A Ds-based gene of interest was linked green fluorescent protein in transfer DNA (T-DNA), protein-aided...
Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies transposon tagging usually slow and labor-intensive yield low frequency tagged lines. We have constructed a series vectors based on three approaches: (i) AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain (GVG) chemical-inducible expression...
Rice blast is one of the most serious diseases rice and a major threat to production. Breeding disease-resistant economical, safe, effective measures for control blast. As complement traditional crop breeding, transgenic method can avoid time-consuming process crosses multi-generation selection. In this study, maize ( Zea mays ) Activator Ac )/Dissociation Ds transposon vectors carrying green fluorescent protein (GFP) red (mCherry) genetic markers were used generating marker-free rice....
Preoperative eye-covering training for 3 hours has been reported to effectively reduce the incidence of emergence delirium (ED) in preschool children. However, most children can only maintain eye being covered less than 60 min, and shortening duration also achieve similar clinical effects as long eye-covering. This study was designed compare 30-min 60-min pretreatment based on cartoon education preoperative anxiety, postoperative ED, pain score after ophthalmic surgery with general...
Abstract Background Long non-coding RNAs (LncRNAs) have emerged as important regulators in many physiological processes plant. By high-throughput RNA-sequencing, pathogen-associated LncRNAs were mapped various plants, and some of them proved to be involved plant defense responses. The rice blast disease caused by Magnaporthe oryzae ( M. ) is one the most destructive diseases rice. However, -induced yet studied. Findings We investigated that associated with fungus. Totally 83 up-regulated...
Abstract Background Long non-coding RNAs (LncRNAs) have emerged as important regulators in many physiological processes plant. By high-throughput RNA-sequencing, pathogen-associated lncRNAs were mapped various plants, and some of them proved to be involved plant defense responses. The rice blast disease caused by Magnaporthe oryzae ( M. ) is one the most destructive diseases rice. However, -induced yet studied. Findings: We investigated that associated with fungus. Totally 83 up-regulated...
Abstract Biotechnologists seeking to develop marker-free transgenic plants or multigene transformants have established different co-transformation methods. To investigate the factors affecting efficiency of T-DNA vectors via mixed Agrobacterium strains and effective strategies for selection desired co-transformants, we utilized GFP RFP as visual markers in rice tested combinations a drug-selectable vector (pGFP-HPT) non-drug selectable (pRFP) two (pGFP-HPT pRFP-HPT). Under similar...
Abstract Background Biotechnologists seeking to develop marker-free transgenic plants have established co-transformation methods. For using mixed Agrobacterium strains, the mix ratio of strains and selection scheme may influence frequency. This study used fluorescent GFP RFP markers compose different schemes for observation selective dynamics transformed rice cells investigate factors affecting efficiency.Methods ResultsWe utilized in tested combinations an antibiotic-selectable vector...