A5025742489- Plant-Microbe Interactions and Immunity
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Mycotoxins in Agriculture and Food
- Plant Pathogenic Bacteria Studies
- Plant Disease Resistance and Genetics
- Polysaccharides and Plant Cell Walls
- Cellular transport and secretion
- Robotic Path Planning Algorithms
- Plant Reproductive Biology
- Mycorrhizal Fungi and Plant Interactions
- Microbial Natural Products and Biosynthesis
- Robotics and Sensor-Based Localization
- Wheat and Barley Genetics and Pathology
- Robotic Mechanisms and Dynamics
- Plant Virus Research Studies
- Plant pathogens and resistance mechanisms
- Plant biochemistry and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Andrographolide Research and Applications
- PARP inhibition in cancer therapy
- Plant Parasitism and Resistance
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
Zhejiang University
2005-2025
Fujian Agriculture and Forestry University
2016-2025
Fudan University
2025
Guangxi Medical University
2025
Association for Language Learning
2024
Taizhou University
2022-2023
National University of Singapore
2023
Community Medical Center
2023
Dalian Jiaotong University
2022
Ministry of Education
2020
The rice blast resistance (R) genes Pi2 and Piz-t confer broad-spectrum against different sets of Magnaporthe grisea isolates. We first identified the gene using a map-based cloning strategy. is member cluster comprising nine members (named Nbs1-Pi2 to Nbs9-Pi2) encodes protein with nucleotide-binding site leucine-rich repeat (LRR) domain. Fine genetic mapping, molecular characterization susceptible mutants, complementation tests indicated that Nbs4-Pi2 gene. gene, allele in cultivar Toride...
The Magnaporthe oryzae avirulence gene AvrPiz-t activates immunity in a gene-for-gene fashion to rice mediated by the blast resistance Piz-t. To dissect molecular mechanism underlying their recognition, we initiated cloning of using map-based strategy. was delimited an approximately 21-kb genomic fragment, which six genes were predicted. Complementation tests each these candidate led final identification AvrPiz-t, encodes 108-amino-acid predicted secreted protein with unknown function and no...
Summary We identified the M agnaporthe oryzae avirulence effector A vr P i9 cognate to rice blast resistance gene by comparative genomics of requisite strains derived from a sequential planting method. AvrPi9 encodes small secreted protein that appears localize in biotrophic interfacial complex and is translocated host cell during infection. forms tandem array with its paralogue proximal centromeric region chromosome 7. expressed highly at early stages initiation disease. Virulent isolate...
We examined the genomes of 100 isolates Magnaporthe oryzae (Pyricularia oryzae), causal agent rice blast disease. grouped current field populations M. into three major globally distributed groups. A genetically diverse group, clade 1, which may represent a group closely related lineages, contains both mating types. Two well-separated clades, clades 2 and 3, appear to have arisen as clonal lineages distinct from clade. Examination genes involved in pathways identified clade-specific...
Rac1 is a small GTPase involved in actin cytoskeleton organization and polarized cell growth many organisms. In this study, we investigate the biological function of MgRac1, homolog Magnaporthe grisea. The Mgrac1 deletion mutants are defective conidial production. Among few conidia generated, they malformed appressorial formation consequently lose pathogenicity. Genetic complementation with native MgRac1 fully recovers all these phenotypes. Consistently, expression dominant negative allele...
Magnaporthe oryzae starts its infection by the attachment of pyriform conidia on rice tissues, and severity disease epidemic is proportional to quantity produced in blast lesions. However, mechanism conidial production not well understood. Homeodomain proteins play critical roles regulating various growth developmental processes fungi other eukaryotes. Through targeted gene replacement, we find that deletion HTF1, one seven homeobox genes fungal genome, does affect mycelial but causes total...
Conidia are primary means of asexual reproduction and dispersal in a variety pathogenic fungi, it is widely recognized that they play critical role animal plant disease epidemics. However, genetic mechanisms associated with conidiogenesis complex remain largely undefined numerous fungi. We previously showed Htf1, homeobox transcription factor, required for the rice pathogen Magnaporthe oryzae. In this study, our aim was to characterize how Htf1 homolog regulates common also distinctive three...
Rab GTPases represent the largest subfamily of Ras-related small and regulate membrane trafficking. Vesicular transport is a general mechanism that governs intracellular trafficking along endocytic exocytic pathways in all eukaryotic cells. Fusarium graminearum filamentous fungus causes devastating economically important head blight wheat related species. The vesicular not well understood, little known about F. graminearum. In this study, we systematically characterized eleven FgRabs by live...
The retromer mediates protein trafficking through recycling cargo from endosomes to the trans-Golgi network in eukaryotes. However, role of such events during pathogen-host interaction remains unclear. Here, we report that cargo-recognition complex (MoVps35, MoVps26 and MoVps29) is essential for appressorium-mediated host penetration by Magnaporthe oryzae, causal pathogen blast disease rice. Loss function blocked glycogen distribution turnover lipid bodies, delayed nuclear degeneration...
One major threat to global food security that requires immediate attention, is the increasing incidence of host shift and expansion in growing number pathogenic fungi emergence new pathogens. The more alarming because, yield quality quantity improvement efforts are encouraging cultivation uniform plants with low genetic diversity increasingly susceptible emerging However, influence genome differentiation on pathogen its contribution adaptability still obscure. Here, we compared sequence 6...
The genome of rice blast fungus (Magnaporthe oryzae) encodes 15 glycoside hydrolase 18 family chitinases. In this study, we characterized the function an M. oryzae extracellular chitinase, MoChi1, and its interaction with a host protein, OsMBL1, jacalin-related Mannose-Binding Lectin (MBL) in (Oryza sativa). Deletion MoChi1 resulted reduced aerial hyphal formation virulence by activating expression defense-responsive genes. We confirmed OsMBL1 vitro vivo. was induced pathogen-associated...
Abstract Background Rice blast is generally considered the most devastating rice disease worldwide. The development of resistant varieties has been proven to be economical strategy control disease. A cluster ( R ) genes on chromosome 12 including Pita, Pita2 and Ptr studies for decades. However, relationship between these not well established. Results In this study, we compared resistance spectra controlled by Pita testing their monogenic lines (MLs) in four hotspots found Philippines...
Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of most devastating diseases for rice crops, significantly affecting crop yield and quality. During infection process, M. oryzae secretes effector proteins that help in hijacking host's immune responses to establish infection. However, little known about interaction between protein AvrPik-D host Pikh, how increases disease severity promote In this study, we show interacts with zinc finger-type transcription factor WG7...
To better understand the molecular basis of defense response against rice blast fungus (Magnaporthe grisea), a large-scale expressed sequence tag (EST) sequencing approach was used to identify genes involved in early infection stages (Oryza sativa). Six cDNA libraries were constructed using infected leaf tissues harvested from 6 conditions: resistant, partially and susceptible reactions at both 24 h after inoculation. Two additional uninoculated leaves lesion mimic mutant spl11. A total...
JUJUNCAO (Cenchrus fungigraminus; 2n = 4x 28) is a Cenchrus grass with the highest biomass production among cultivated plants, and it can be used for mushroom cultivation, animal feed, biofuel production. Here, we report nearly complete genome assembly of reveal that an allopolyploid originated ∼2.7 million years ago (mya). Its consists two subgenomes, subgenome A shares high collinear synteny pearl millet. We also investigated evolution suggest ancestral karyotype split into B karyotypes...
Unique genes refer to specific a particular organism and play crucial roles in the biological functions, evolutionary processes, adaptations external environments. However, of unique plant pathogenic fungi remain largely unexplored. In this study, we identified novel gene rice blast fungus Magnaporthe oryzae, named MoUNG (M. oryzae gene), through T-DNA insertion mutagenesis. The disruption promoter region mutant (T30-104) led an almost loss expression. has no functional domains lacks...
Protein phosphatases are crucial enzymes that regulate key cellular processes such as the cell cycle, gene transcription, and translation in eukaryotes. Seven PP2C protein have been identified Magnaporthe oryzae. However, their synergistic roles pathology physiology of M. oryzae remain poorly investigated. By qRT-PCR analysis, we found PTC1 PTC2 significantly upregulated PTC5 deletion mutant. The double MoPTC5/MoPTC1 MoPTC5/MoPTC2 genes reduced hyphal growth, conidiophore formation,...